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Article

Leptin promotes the proliferation and migration of human breast cancer through the extracellular-signal regulated kinase pathway

  • Authors:
    • Hong-Jun Yuan
    • Ke-Wang Sun
    • Kun Yu
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang 310014, P.R. China
  • Pages: 350-354
    |
    Published online on: November 8, 2013
       https://doi.org/10.3892/mmr.2013.1786
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Abstract

Obesity has been associated with an increased risk of postmenopausal breast cancer, which may be due to the expression of leptin. The aim of this study was to determine the role of leptin in the growth of breast cancer cells in nude mice, the proliferation and migration of MCF-7 human breast cancer cells and its downstream signaling pathway. The xenograft mouse model was elicited by injecting MCF-7 human breast cancer cells into the left back axilla and the tumor size was measured every other day. Leptin injected subcutaneously around the tumor site led to an increase in the size and weight of the tumor, whereas the leptin antagonist (LA) significantly inhibited the size and weight of the tumor. Leptin promoted the proliferation and migration of MCF-7 cells and LA inhibited it. The effects of leptin on increasing the size and weight of the tumor in the nude mice and the proliferation and migration of MCF-7 human breast cancer cells were eradicated by pretreatment with LA, the extracellular-signal regulated kinase (ERK) inhibitor PD98059. In the xenograft mouse model the leptin level was increased and leptin increased the phosphorylation of ERK in the MCF-7 cells, whereas LA significantly reduced the phosphorylation of ERK. These results indicated that leptin promotes the growth of breast cancer in the nude mice and increases the proliferation and migration of MCF-7 human breast cancer cells via the ERK pathway.
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1 

Jemal A, Siegel R, Xu J and Ward E: Cancer statistics, 2010. CA Cancer J Clin. 60:277–300. 2010. View Article : Google Scholar

2 

Cao W, Wang X and Li JC: Hereditary breast cancer in the Han Chinese population. J Epidemiol. 2:75–84. 2013. View Article : Google Scholar : PubMed/NCBI

3 

Kobayashi S, Sugiura H, Ando Y, Shiraki N, Yanagi T, Yamashita H and Toyama T: Reproductive history and breast cancer risk. Breast Cancer. 19:302–308. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Hauner D, Janni W, Rack B and Hauner H: The effect of overweight and nutrition on prognosis in breast cancer. Dtsch Arztebl Int. 108:795–801. 2011.PubMed/NCBI

5 

Irwin ML, Alvarez-Reeves M, Cadmus L, et al: Exercise improves body fat, lean mass, and bone mass in breast cancer survivors. Obesity (Silver Spring). 17:1534–1541. 2009. View Article : Google Scholar : PubMed/NCBI

6 

Schmitz KH, Ahmed RL, Hannan PJ and Yee D: Safety and efficacy of weight training in recent breast cancer survivors to alter body composition, insulin, and insulin-like growth factor axis proteins. Cancer Epidemiol Biomarkers Prev. 14:1672–1680. 2005. View Article : Google Scholar : PubMed/NCBI

7 

Demark-Wahnefried W, Campbell KL and Hayes SC: Weight management and its role in breast cancer rehabilitation. Cancer. 118:2277–2287. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Garofalo C and Surmacz E: Leptin and cancer. J Cell Physiol. 207:12–22. 2006. View Article : Google Scholar

9 

Zhang Y, Proenca R, Maffei M, Barone M, Leopold L and Friedman JM: Positional cloning of the mouse obese gene and its human homologue. Nature. 372:425–432. 1994. View Article : Google Scholar : PubMed/NCBI

10 

Kontos A, de Menezes RC, Ootsuka Y and Blessing W: Brown adipose tissue thermogenesis precedes food intake in genetically obese Zucker (fa/fa) rats. Physiol Behav. 118:129–137. 2013. View Article : Google Scholar : PubMed/NCBI

11 

Harris RB: Direct and indirect effects of leptin on adipocyte metabolism. Biochim Biophys Acta. May 17–2013.(Epub ahead of print).

12 

Dong M and Ren J: What fans the fire: insights into mechanisms of leptin in metabolic syndrome-associated heart diseases. Curr Pharm Des. May 14–2013.(Epub ahead of print).

13 

Simonds SE, Cowley MA and Enriori PJ: Leptin increasing sympathetic nerve outflow in obesity: A cure for obesity or a potential contributor to metabolic syndrome? Adipocyte. 1:177–181. 2012. View Article : Google Scholar : PubMed/NCBI

14 

Bouchard C, Tchernof A and Tremblay A: Predictors of body composition and body energy changes in response to chronic overfeeding. Int J Obes (Lond). May 20–2013.(Epub ahead of print).

15 

Ide S, Tokuyama R, Davaadorj P, Shimozuma M, Kumasaka S, Tatehara S and Satomura K: Leptin and vascular endothelial growth factor regulate angiogenesis in tooth germs. Histochem Cell Biol. 135:281–292. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Singireddy AV, Inglis MA, Zuure WA, Kim JS and Anderson GM: Neither signal transducer and activator of transcription 3 (STAT3) or STAT5 signaling pathways are required for leptin’s effects on fertility in mice. Endocrinology. 154:2434–2445. 2013.

17 

Singh UP, Singh NP, Guan H, et al: Leptin antagonist ameliorates chronic colitis in IL-10 −/− mice. Immunobiology. May 13–2013.(Epub ahead of print).

18 

Alokail MS, Al-Daghri N, Abdulkareem A, Draz HM, Yakout SM, Alnaami AM, Sabico S, Alenad AM and Chrousos GP: Metabolic syndrome biomarkers and early breast cancer in Saudi women: evidence for the presence of a systemic stress response and/or a pre-existing metabolic syndrome-related neoplasia risk? BMC Cancer. 13:542013. View Article : Google Scholar

19 

Fiorio E, Mercanti A, Terrasi M, et al: Leptin/HER2 crosstalk in breast cancer: in vitro study and preliminary in vivo analysis. BMC Cancer. 8:3052008. View Article : Google Scholar : PubMed/NCBI

20 

Bouret SG, Bates SH, Chen S, Myers MG Jr and Simerly RB: Distinct roles for specific leptin receptor signals in the development of hypothalamic feeding circuits. J Neurosci. 32:1244–1252. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Elias CF and Purohit D: Leptin signaling and circuits in puberty and fertility. Cell Mol Life Sci. 70:841–862. 2013. View Article : Google Scholar : PubMed/NCBI

22 

Liu Z, Uesaka T, Watanabe H and Kato N: High fat diet enhances colonic cell proliferation and carcinogenesis in rats by elevating serum leptin. Int J Oncol. 19:1009–1014. 2001.PubMed/NCBI

23 

Hardwick JC, Van Den Brink GR, Offerhaus GJ, Van Deventer SJ and Peppelenbosch MP: Leptin is a growth factor for colonic epithelial cells. Gastroenterology. 121:79–90. 2001. View Article : Google Scholar : PubMed/NCBI

24 

Rouet-Benzineb P, Aparicio T, Guilmeau S, Pouzet C, Descatoire V, Buyse M and Bado A: Leptin counteracts sodium butyrate-induced apoptosis in human colon cancer HT-29 cells via NF-kappaB signaling. J Biol Chem. 279:16495–16502. 2004. View Article : Google Scholar : PubMed/NCBI

25 

Somasundar P, Frankenberry KA, Skinner H, et al: Prostate cancer cell proliferation is influenced by leptin. J Surg Res. 118:71–82. 2004. View Article : Google Scholar : PubMed/NCBI

26 

Yu W, Gu JC, Liu JZ, et al: Regulation of estrogen receptors alpha and beta in human breast carcinoma by exogenous leptin in nude mouse xenograft model. Chin Med J (Engl). 123:337–343. 2010.PubMed/NCBI

27 

Savry A, Carre M, Berges R, Rovini A, Pobel I, Chacon C, Braguer D and Bourgarel-Rey V: Bcl-2-enhanced efficacy of microtubule-targeting chemotherapy through Bim overexpression: implications for cancer treatment. Neoplasia. 15:49–60. 2013.PubMed/NCBI

28 

Sinicrope FA and Dannenberg AJ: Obesity and breast cancer prognosis: weight of the evidence. J Clin Oncol. 29:4–7. 2011. View Article : Google Scholar : PubMed/NCBI

29 

van den Brandt PA, Spiegelman D, Yaun SS, et al: Pooled analysis of prospective cohort studies on height, weight, and breast cancer risk. Am J Epidemiol. 152:514–527. 2000.PubMed/NCBI

30 

Key TJ, Appleby PN, Reeves GK, et al: Body mass index, serum sex hormones, and breast cancer risk in postmenopausal women. J Natl Cancer Inst. 95:1218–1226. 2003. View Article : Google Scholar : PubMed/NCBI

31 

Hu X, Juneja SC, Maihle NJ and Cleary MP: Leptin - a growth factor in normal and malignant breast cells and for normal mammary gland development. J Natl Cancer Inst. 94:1704–1711. 2002. View Article : Google Scholar

32 

Dieudonne MN, Machinal-Quelin F, Serazin-Leroy V, Leneveu MC, Pecquery R and Giudicelli Y: Leptin mediates a proliferative response in human MCF7 breast cancer cells. Biochem Biophys Res Commun. 293:622–628. 2002. View Article : Google Scholar : PubMed/NCBI

33 

Rene Gonzalez R, Watters A, Xu Y, Singh UP, Mann DR, Rueda BR and Penichet ML: Leptin-signaling inhibition results in efficient anti-tumor activity in estrogen receptor positive or negative breast cancer. Breast Cancer Res. 11:R362009.

34 

D’Esposito V, Passaretti F, Hammarstedt A, et al: Adipocyte-released insulin-like growth factor-1 is regulated by glucose and fatty acids and controls breast cancer cell growth in vitro. Diabetologia. 55:2811–2822. 2012.PubMed/NCBI

35 

Grisouard J, Dembinski K, Mayer D, Keller U, Muller B and Christ-Crain M: Targeting AMP-activated protein kinase in adipocytes to modulate obesity-related adipokine production associated with insulin resistance and breast cancer cell proliferation. Diabetol Metab Syndr. 3:162011. View Article : Google Scholar

36 

Kim EJ, Choi MR, Park H, et al: Dietary fat increases solid tumor growth and metastasis of 4T1 murine mammary carcinoma cells and mortality in obesity-resistant BALB/c mice. Breast Cancer Res. 13:R782011. View Article : Google Scholar : PubMed/NCBI

37 

Barone I, Catalano S, Gelsomino L, et al: Leptin mediates tumor-stromal interactions that promote the invasive growth of breast cancer cells. Cancer Res. 72:1416–1427. 2012. View Article : Google Scholar : PubMed/NCBI

38 

Jung DW, Che ZM and Kim J, Kim K, Kim KY, Williams D and Kim J: Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7. Int J Cancer. 127:332–344. 2010.PubMed/NCBI

39 

Kumar S, Kishimoto H, Chua HL, et al: Interleukin-1 alpha promotes tumor growth and cachexia in MCF-7 xenograft model of breast cancer. Am J Pathol. 163:2531–2541. 2003. View Article : Google Scholar : PubMed/NCBI

40 

Li L, Dragulev B, Zigrino P, Mauch C and Fox JW: The invasive potential of human melanoma cell lines correlates with their ability to alter fibroblast gene expression in vitro and the stromal microenvironment in vivo. Int J Cancer. 125:1796–1804. 2009. View Article : Google Scholar : PubMed/NCBI

41 

Robertson SA, Leinninger GM and Myers MG Jr: Molecular and neural mediators of leptin action. Physiol Behav. 94:637–642. 2008. View Article : Google Scholar : PubMed/NCBI

42 

Frankenberry KA, Skinner H, Somasundar P, McFadden DW and Vona-Davis LC: Leptin receptor expression and cell signaling in breast cancer. Int J Oncol. 28:985–993. 2006.PubMed/NCBI

43 

Chen H, Zhang ZW, Guo Y, Wang Y, Liu Y, Luo N and Zhu Y: The proliferative role of insulin and the mechanism underlying this action in human breast cancer cell line MCF-7. J BUON. 17:658–662. 2012.PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Yuan H, Sun K and Yu K: Leptin promotes the proliferation and migration of human breast cancer through the extracellular-signal regulated kinase pathway. Mol Med Rep 9: 350-354, 2014.
APA
Yuan, H., Sun, K., & Yu, K. (2014). Leptin promotes the proliferation and migration of human breast cancer through the extracellular-signal regulated kinase pathway. Molecular Medicine Reports, 9, 350-354. https://doi.org/10.3892/mmr.2013.1786
MLA
Yuan, H., Sun, K., Yu, K."Leptin promotes the proliferation and migration of human breast cancer through the extracellular-signal regulated kinase pathway". Molecular Medicine Reports 9.1 (2014): 350-354.
Chicago
Yuan, H., Sun, K., Yu, K."Leptin promotes the proliferation and migration of human breast cancer through the extracellular-signal regulated kinase pathway". Molecular Medicine Reports 9, no. 1 (2014): 350-354. https://doi.org/10.3892/mmr.2013.1786
Copy and paste a formatted citation
x
Spandidos Publications style
Yuan H, Sun K and Yu K: Leptin promotes the proliferation and migration of human breast cancer through the extracellular-signal regulated kinase pathway. Mol Med Rep 9: 350-354, 2014.
APA
Yuan, H., Sun, K., & Yu, K. (2014). Leptin promotes the proliferation and migration of human breast cancer through the extracellular-signal regulated kinase pathway. Molecular Medicine Reports, 9, 350-354. https://doi.org/10.3892/mmr.2013.1786
MLA
Yuan, H., Sun, K., Yu, K."Leptin promotes the proliferation and migration of human breast cancer through the extracellular-signal regulated kinase pathway". Molecular Medicine Reports 9.1 (2014): 350-354.
Chicago
Yuan, H., Sun, K., Yu, K."Leptin promotes the proliferation and migration of human breast cancer through the extracellular-signal regulated kinase pathway". Molecular Medicine Reports 9, no. 1 (2014): 350-354. https://doi.org/10.3892/mmr.2013.1786
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