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Article

Annexin II mediates the neutrophil elastase-stimulated exocytosis of mucin 5ac

  • Authors:
    • Rui Xu
    • Qi Li
    • Xiangdong Zhou
    • Juliy M. Perelman
    • Victor P. Kolosov
  • View Affiliations / Copyright

    Affiliations: Department of Respiratory Medicine, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, P.R. China, Far Eastern Scientific Center of Physiology and Pathology of Respiration, Russian Academy of Medical Sciences, Blagoveschensk, Amur Oblast 675000, Russia
  • Pages: 299-304
    |
    Published online on: November 14, 2013
       https://doi.org/10.3892/mmr.2013.1795
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Abstract

The overexpression and hypersecretion of mucus is a hallmark of several chronic pulmonary inflammatory diseases, including chronic obstructive pulmonary disease (COPD), asthma and cystic fibrosis. Mucin 5ac (MUC5AC) is a major component of airway mucus. Annexin II (ANXII) has been reported to be expressed in various cells and is associated with the fusion of secretory vesicles. Neutrophil elastase (NE) is present at high concentrations in the airway surface fluid in patients with cystic fibrosis and various other severe diseases. However, the role of ANXII in NE-induced secretion of MUC5AC granules remains unclear. It was determined that NE upregulates the transcription and protein synthesis of ANXII in 16HBE human bronchial epithelial cells. Following stimulation with NE, ANXII is recruited to the cell membrane, as visualised by cell immunochemistry and laser confocal microscopy, and the redistribution of ANXII is inhibited by the protein kinase-C (PKC) inhibitor bisindolylmaleimide I. Conversely, depleting endogenous ANXII decreases MUC5AC secretion into the cell culture supernatant and increases the levels of intracellular MUC5AC protein. The data indicated that ANXII is associated with the secretion of MUC5AC granules.
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Copy and paste a formatted citation
Spandidos Publications style
Xu R, Li Q, Zhou X, Perelman JM and Kolosov VP: Annexin II mediates the neutrophil elastase-stimulated exocytosis of mucin 5ac. Mol Med Rep 9: 299-304, 2014.
APA
Xu, R., Li, Q., Zhou, X., Perelman, J.M., & Kolosov, V.P. (2014). Annexin II mediates the neutrophil elastase-stimulated exocytosis of mucin 5ac. Molecular Medicine Reports, 9, 299-304. https://doi.org/10.3892/mmr.2013.1795
MLA
Xu, R., Li, Q., Zhou, X., Perelman, J. M., Kolosov, V. P."Annexin II mediates the neutrophil elastase-stimulated exocytosis of mucin 5ac". Molecular Medicine Reports 9.1 (2014): 299-304.
Chicago
Xu, R., Li, Q., Zhou, X., Perelman, J. M., Kolosov, V. P."Annexin II mediates the neutrophil elastase-stimulated exocytosis of mucin 5ac". Molecular Medicine Reports 9, no. 1 (2014): 299-304. https://doi.org/10.3892/mmr.2013.1795
Copy and paste a formatted citation
x
Spandidos Publications style
Xu R, Li Q, Zhou X, Perelman JM and Kolosov VP: Annexin II mediates the neutrophil elastase-stimulated exocytosis of mucin 5ac. Mol Med Rep 9: 299-304, 2014.
APA
Xu, R., Li, Q., Zhou, X., Perelman, J.M., & Kolosov, V.P. (2014). Annexin II mediates the neutrophil elastase-stimulated exocytosis of mucin 5ac. Molecular Medicine Reports, 9, 299-304. https://doi.org/10.3892/mmr.2013.1795
MLA
Xu, R., Li, Q., Zhou, X., Perelman, J. M., Kolosov, V. P."Annexin II mediates the neutrophil elastase-stimulated exocytosis of mucin 5ac". Molecular Medicine Reports 9.1 (2014): 299-304.
Chicago
Xu, R., Li, Q., Zhou, X., Perelman, J. M., Kolosov, V. P."Annexin II mediates the neutrophil elastase-stimulated exocytosis of mucin 5ac". Molecular Medicine Reports 9, no. 1 (2014): 299-304. https://doi.org/10.3892/mmr.2013.1795
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