Silencing Kif2a induces apoptosis in squamous cell carcinoma of the oral tongue through inhibition of the PI3K/Akt signaling pathway

Wang,Ketao; Lin,Changlong; Wang,Chengqin; Shao,Qianqian; Gao,Wenjuan; Song,Bingfeng; Wang,Lei; Song,Xiaobin; Qu,Xun; Wei,Fengcai

doi:10.3892/mmr.2013.1804

2014-January Volume 9 Issue 1

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2014-January Volume 9 Issue 1

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Article

Silencing Kif2a induces apoptosis in squamous cell carcinoma of the oral tongue through inhibition of the PI3K/Akt signaling pathway

Authors:
- Ketao Wang
- Changlong Lin
- Chengqin Wang
- Qianqian Shao
- Wenjuan Gao
- Bingfeng Song
- Lei Wang
- Xiaobin Song
- Xun Qu
- Fengcai Wei
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Affiliations: Department of Oral and Maxillofacial Surgery and Institution of Dental Medicine, Qilu Hospital, Shandong University, Jinan, Shandong 250012, P.R. China, Department of Pathology, Medical College of Qingdao University, Qingdao, Shandong 266000, P.R. China, Institute of Basic Medical Sciences, Qilu Hospital, Shandong University, Jinan, Shandong 250012, P.R. China
Pages: 273-278
|
Published online on: November 15, 2013

https://doi.org/10.3892/mmr.2013.1804
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Abstract

Previous studies have demonstrated that the overexpression of Kif2a is involved in the progression, invasion and metastasis of squamous cell carcinoma of the oral tongue (SCCOT). Few studies have reported the correlation between Kif2a and apoptosis of tumor cells and which signaling pathways Kif2a is involved in remains unclear. The phosphatidylinositol‑3‑kinase (PI3K)/protein kinase B (Akt) pathway is frequently activated in many types of human cancer. The aim of the present study was to investigate the effects of downregulation of Kif2 expression on the P13K/Akt pathway in Tca8113 cells to determine whether silencing of Kif2 inhibits the P13K/Akt pathway, resulting in cell apoptosis. siRNA vector was constructed, western blot analysis was used to determine RNA interference and flow cytometry was used to determine promotion of Tca8113 cell apoptosis. The results revealed that silencing Kif2a induces apoptosis and decreases the mRNA and protein level of PI3K, Akt and B‑cell lymphoma 2 (Bcl‑2) in Tca8113 cells. The PI3K-specific agonist insulin‑like growth factor 1 (IGF‑1) eliminated the upregulation of apoptosis of Tca8113‑Kif2a cells by phosphorylation of Akt. The results suggest that silencing Kif2a induces tumor cell apoptosis, at least partially, through the PI3K/Akt signaling pathway.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Silencing Kif2a induces apoptosis in squamous cell carcinoma of the oral tongue through inhibition of the PI3K/Akt signaling pathway

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