P2X7 blockade attenuates mouse liver fibrosis

Huang,Changshan; Yu,Wei; Cui,Hong; Wang,Yunjian; Zhang,Ling; Han,Feng; Huang,Tao

doi:10.3892/mmr.2013.1807

2014-January Volume 9 Issue 1

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2014-January Volume 9 Issue 1

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Article

P2X7 blockade attenuates mouse liver fibrosis

Authors:
- Changshan Huang
- Wei Yu
- Hong Cui
- Yunjian Wang
- Ling Zhang
- Feng Han
- Tao Huang
View Affiliations / Copyright

Affiliations: Department of Hepatobiliary Surgery, Henan Provincial Cancer Hospital, Zhengzhou, Henan 450008, P.R. China
Pages: 57-62
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Published online on: November 18, 2013

https://doi.org/10.3892/mmr.2013.1807
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Abstract

P2X7 is important in inflammation and tissue injury. The aim of the present study was to investigate the effect of P2X7 inhibition, using a specific inhibitor (A438079) to prevent the development of liver injury and fibrosis in a mouse model of liver fibrosis. The mouse liver fibrosis model was induced by carbon tetrachloride (CCl4). Mice received subcutaneous administration of vehicle (saline/olive oil), CCl4 or subcutaneous CCl4 and A438079. The pro‑inflammatory and pro‑fibrotic factors were determined by western blot analysis. The biochemistry, histopathology, collagen deposition and nuclear factor‑κB (NF‑κB) activity were also analyzed. Chronic CCl4 treatment resulted in liver injury and collagen accumulation. The expression levels of P2X7, pro‑inflammatory and pro‑fibrotic mediators, and the activity of NF‑κB were markedly increased. Treatment with A438079 significantly inhibited CCl4‑induced P2X7 expression, and attenuated CCl4‑induced liver injury and the inflammatory response. P2X7 blockade also significantly reduced the formation of collagen in the liver and the expression of α-smooth muscle actin and transforming growth factor‑β1. This study demonstrated that P2X7 inhibition attenuated liver injury and fibrosis in a mouse model. Thus, P2X7 is a potential novel therapeutic target for liver injury and fibrosis.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

P2X7 blockade attenuates mouse liver fibrosis

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