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Molecular Medicine Reports
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Article Open Access

Functional expression, characterization and application of the human S100A4 protein

  • Authors:
    • Degang Wang
    • Jianwei Zhang
    • Ziquan Liu
    • Yunyun Chen
    • Chuanxiang Xu
    • Zhiqing Zhang
    • Xiaohua Liu
    • Lei Wu
    • Xuesi Zhou
    • Xiangyan Meng
    • Hua Li
    • Hongtao Liu
    • Zifeng Jiang
    • Tianhui Wang

  • View Affiliations / Copyright

    Affiliations: Performance Medicine Laboratory, Institute of Health and Environmental Medicine, Tianjin 300050, P.R. China, Endoscopy Division, Tianjin Medical University Cancer Hospital and City Key Laboratory of Tianjin Cancer Center, Tianjin 300060, P.R. China, Division of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences, Chaoyang, Beijing 100101, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 175-181
    |
    Published online on: October 22, 2014
       https://doi.org/10.3892/mmr.2014.2745
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Abstract

Preparations utilizing monoclonal antibodies against S100A4 provide useful tools for functional studies to investigate the clinical applications of the human S100A4 protein. In the present study, human S100A4 protein was expressed in Escherichia coli (E. coli) BL21 (DE3), successfully purified by diethylaminoethyl cellulose anion-exchange chromatography and identified by western blot analysis. Soluble S100A4 bioactivity was confirmed by Transwell migration and invasion assays in the human HeLa cell line. Monoclonal antibodies (mAbs) were generated utilizing the standard hybridoma method and were validated by enzyme-linked immunosorbent assay and western blot analysis. The antibody was then used to examine human gastric carcinoma specimens by immunohistochemistry. Recombinant S100A4 was functionally expressed in E. coli and promoted the migration and invasion of HeLa cells. Four hybridoma cell lines, which secreted mAbs specifically against human S100A4 protein, were obtained. One of the four mAbs, namely 2A12D10B2, recognized human S100A4 as indicated by immunohistochemical staining of human gastric carcinoma specimens and recombinant S100A4 was functionally expressed in E. coli. The mAbs of recombinant S100A4 were suitable for detecting S100A4 expression in human tissues and for investigating the subsequent clinical applications of the protein.

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Copy and paste a formatted citation
Spandidos Publications style
Wang D, Zhang J, Liu Z, Chen Y, Xu C, Zhang Z, Liu X, Wu L, Zhou X, Meng X, Meng X, et al: Functional expression, characterization and application of the human S100A4 protein. Mol Med Rep 11: 175-181, 2015.
APA
Wang, D., Zhang, J., Liu, Z., Chen, Y., Xu, C., Zhang, Z. ... Wang, T. (2015). Functional expression, characterization and application of the human S100A4 protein. Molecular Medicine Reports, 11, 175-181. https://doi.org/10.3892/mmr.2014.2745
MLA
Wang, D., Zhang, J., Liu, Z., Chen, Y., Xu, C., Zhang, Z., Liu, X., Wu, L., Zhou, X., Meng, X., Li, H., Liu, H., Jiang, Z., Wang, T."Functional expression, characterization and application of the human S100A4 protein". Molecular Medicine Reports 11.1 (2015): 175-181.
Chicago
Wang, D., Zhang, J., Liu, Z., Chen, Y., Xu, C., Zhang, Z., Liu, X., Wu, L., Zhou, X., Meng, X., Li, H., Liu, H., Jiang, Z., Wang, T."Functional expression, characterization and application of the human S100A4 protein". Molecular Medicine Reports 11, no. 1 (2015): 175-181. https://doi.org/10.3892/mmr.2014.2745
Copy and paste a formatted citation
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Spandidos Publications style
Wang D, Zhang J, Liu Z, Chen Y, Xu C, Zhang Z, Liu X, Wu L, Zhou X, Meng X, Meng X, et al: Functional expression, characterization and application of the human S100A4 protein. Mol Med Rep 11: 175-181, 2015.
APA
Wang, D., Zhang, J., Liu, Z., Chen, Y., Xu, C., Zhang, Z. ... Wang, T. (2015). Functional expression, characterization and application of the human S100A4 protein. Molecular Medicine Reports, 11, 175-181. https://doi.org/10.3892/mmr.2014.2745
MLA
Wang, D., Zhang, J., Liu, Z., Chen, Y., Xu, C., Zhang, Z., Liu, X., Wu, L., Zhou, X., Meng, X., Li, H., Liu, H., Jiang, Z., Wang, T."Functional expression, characterization and application of the human S100A4 protein". Molecular Medicine Reports 11.1 (2015): 175-181.
Chicago
Wang, D., Zhang, J., Liu, Z., Chen, Y., Xu, C., Zhang, Z., Liu, X., Wu, L., Zhou, X., Meng, X., Li, H., Liu, H., Jiang, Z., Wang, T."Functional expression, characterization and application of the human S100A4 protein". Molecular Medicine Reports 11, no. 1 (2015): 175-181. https://doi.org/10.3892/mmr.2014.2745
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