Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
May-2015 Volume 11 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
May-2015 Volume 11 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Lipopolysaccharide stimulation improves the odontoblastic differentiation of human dental pulp cells

  • Authors:
    • Yihua Huang
    • Hongwei Jiang
    • Qimei Gong
    • Xuyan Li
    • Junqi Ling
  • View Affiliations / Copyright

    Affiliations: Guangdong Provincial Key Laboratory of Stomatology, Department of Operative Dentistry and Endodontics, Guanghua School and Hospital of Stomatology, Sun Yat‑sen University, Guangzhou, Guangdong 510055, P.R. China
  • Pages: 3547-3552
    |
    Published online on: December 19, 2014
       https://doi.org/10.3892/mmr.2014.3120
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Lipopolysaccharide (LPS) is one of the causative agents of pulpitis and previous studies have demonstrated that the LPS stimulation of human aortic valve interstitial cells induces inflammatory mediators and the gene expression of osteogenic factors. Therefore, in the present study, it was hypothesized that LPS affects the odontoblastic differentiation of human dental pulp cells (hDPCs). In order to investigate this, an in vitro study using hDPCs was performed. Increased alkaline phosphatase (ALP) activity was observed in the hDPCs treated with LPS, which was more marked when the cells were costimulated with odontogenic induction medium (OM). LPS also appeared to increase the gene expression levels of dentin sialophosphoprotein and dentin matrix protein‑1 and the protein expression level of dental sialoprotein in the hDPCs, particularly in combination with OM. In addition, the size and the number of nodules formed in the hDPCs exposed to OM and LPS were increased compared to those stimulated by OM alone. To determine the role of nuclear factor κB (NF‑κB) during the LPS‑induced odontoblastic differentiation of hDPCs, immunofluorescence was performed. The nuclear translocation of NF‑κB, induced by LPS was confirmed, suggesting its involvement in the LPS‑induced increase in odontoblastic differentiation of hDPCs. In conclusion, there may be an association between LPS stimulation, with or without OM, and odontoblastic differentiation.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Gronthos S, Brahim J, Li W, et al: Stem cell properties of human dental pulp stem cells. J Dent Res. 81:531–535. 2002. View Article : Google Scholar : PubMed/NCBI

2 

Goldberg M, Farges JC, Lacerda-Pinheiro S, et al: Inflammatory and immunological aspects of dental pulp repair. Pharmacol Res. 58:137–147. 2008. View Article : Google Scholar : PubMed/NCBI

3 

Wei X, Ling J, Wu L, Liu L and Xiao Y: Expression of mineralization markers in dental pulp cells. J Endod. 33:703–708. 2007. View Article : Google Scholar : PubMed/NCBI

4 

Wei X, Wu L, Ling J, et al: Differentially expressed protein profile of human dental pulp cells in the early process of odontoblast-like differentiation in vitro. J Endod. 34:1077–1084. 2008. View Article : Google Scholar : PubMed/NCBI

5 

Tobias PS, Mathison JC and Ulevitch RJ: A family of lipopolysaccharide binding proteins involved in responses to gram-negative sepsis. J Biol Chem. 263:13479–13481. 1988.PubMed/NCBI

6 

Vernooy JH, Dentener MA, van Suylen RJ, Buurman WA and Wouters EF: Long-term intratracheal lipopolysaccharide exposure in mice results in chronic lung inflammation and persistent pathology. Am J Respir Cell Mol Biol. 26:152–159. 2002. View Article : Google Scholar

7 

Hanazawa S, Nakada K, Ohmori Y, Miyoshi T, Amano S and Kitano S: Functional role of interleukin 1 in periodontal disease: induction of interleukin 1 production by Bacteroides gingivalis lipopolysaccharide in peritoneal macrophages from C3H/HeN and C3H/HeJ mice. Infect Immun. 50:262–270. 1985.PubMed/NCBI

8 

Vianna ME, Horz HP, Conrads G, Zaia AA, Souza-Filho FJ and Gomes BP: Effect of root canal procedures on endotoxins and endodontic pathogens. Oral Microbiol Immunol. 22:411–418. 2007. View Article : Google Scholar : PubMed/NCBI

9 

Hoshino K, Takeuchi O, Kawai T, et al: Cutting edge: Toll-like receptor 4 (TLR4)-deficient mice are hyporesponsive to lipopolysaccharide: evidence for TLR4 as the Lps gene product. J Immunol. 162:3749–3752. 1999.PubMed/NCBI

10 

Yang X, Coriolan D, Murthy V, Schultz K, Golenbock DT and Beasley D: Proinflammatory phenotype of vascular smooth muscle cells: role of efficient Toll-like receptor 4 signaling. Am J Physiol Heart Circ Physiol. 289:H1069–H1076. 2005. View Article : Google Scholar : PubMed/NCBI

11 

Jiang HW, Zhang W, Ren BP, Zeng JF and Ling JQ: Expression of toll like receptor 4 in normal human odontoblasts and dental pulp tissue. J Endod. 32:747–751. 2006. View Article : Google Scholar : PubMed/NCBI

12 

Meng X, Ao L, Song Y, et al: Expression of functional Toll-like receptors 2 and 4 in human aortic valve interstitial cells: potential roles in aortic valve inflammation and stenosis. Am J Physiol Cell Physiol. 294:C29–C35. 2008. View Article : Google Scholar

13 

Yang X, Fullerton DA, Su X, Ao L and Meng X: Pro-osteogenic phenotype of human aortic valve interstitial cells is associated with higher levels of Toll-like receptors 2 and 4 and enhanced expression of bone morphogenetic protein 2. J Am Coll Cardiol. 53:491–500. 2009. View Article : Google Scholar : PubMed/NCBI

14 

O’Neill LA and Bowie AG: The family of five: TIR-domain-containing adaptors in Toll-like receptor signalling. Nat Rev Immunol. 7:353–364. 2007. View Article : Google Scholar

15 

Lee JC, Yu MK, Lee R, et al: Terrein reduces pulpal inflammation in human dental pulp cells. J Endod. 34:433–437. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Wang J, Wei X, Ling J, Huang Y and Gong Q: Side population increase after simulated transient ischemia in human dental pulp cell. J Endod. 36:453–458. 2010. View Article : Google Scholar : PubMed/NCBI

17 

Jiang HW, Ling JQ and Gong QM: The expression of stromal cell-derived factor 1 (SDF-1) in inflamed human dental pulp. J Endod. 34:1351–1354. 2008. View Article : Google Scholar : PubMed/NCBI

18 

Yamada Y, Fujimoto A, Ito A, et al: Cluster analysis and gene expression profiles: a cDNA microarray system-based comparison between human dental pulp stem cells (hDPCs) and human mesenchymal stem cells (hMSCs) for tissue engineering cell therapy. Biomaterials. 27:3766–3781. 2006. View Article : Google Scholar : PubMed/NCBI

19 

Shiba H, Mouri Y, Komatsuzawa H, et al: Enhancement of alkaline phosphatase synthesis in pulp cells co-cultured with epithelial cells derived from lower rabbit incisors. Cell Biol Int. 27:815–823. 2003. View Article : Google Scholar : PubMed/NCBI

20 

Paine ML, Luo W, Wang HJ, et al: Dentin sialoprotein and dentin phosphoprotein overexpression during amelogenesis. J Biol Chem. 280:31991–31998. 2005. View Article : Google Scholar : PubMed/NCBI

21 

Begue-Kirn C, Krebsbach PH, Bartlett JD and Butler WT: Dentin sialoprotein, dentin phosphoprotein, enamelysin and ameloblastin: tooth-specific molecules that are distinctively expressed during murine dental differentiation. Eur J Oral Sci. 106:963–970. 1998. View Article : Google Scholar : PubMed/NCBI

22 

Babu AN, Meng X, Zou N, et al: Lipopolysaccharide stimulation of human aortic valve interstitial cells activates inflammation and osteogenesis. Ann Thorac Surg. 86:71–76. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Lopez J, Fernandez-Pisonero I, Duenas AI, et al: Viral and bacterial patterns induce TLR-mediated sustained inflammation and calcification in aortic valve interstitial cells. Int J Cardiol. 158:18–25. 2012. View Article : Google Scholar

24 

Yamagishi VT, Torneck CD, Friedman S, Huang GT and Glogauer M: Blockade of TLR2 inhibits Porphyromonas gingivalis suppression of mineralized matrix formation by human dental pulp stem cells. J Endod. 37:812–818. 2011. View Article : Google Scholar : PubMed/NCBI

25 

Janssens S and Beyaert R: A universal role for MyD88 in TLR/IL-1R-mediated signaling. Trends Biochem Sci. 27:474–482. 2002. View Article : Google Scholar : PubMed/NCBI

26 

Chang J, Zhang C, Tani-Ishii N, Shi S and Wang CY: NF-κB activation in human dental pulp stem cells by TNF and LPS. J Dent Res. 84:994–998. 2005. View Article : Google Scholar : PubMed/NCBI

27 

He W, Qu T, Yu Q, et al: LPS induces IL-8 expression through TLR4, MyD88, NF-kappaB and MAPK pathways in human dental pulp stem cells. Int Endod J. 46:128–136. 2013. View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Huang Y, Jiang H, Gong Q, Li X and Ling J: Lipopolysaccharide stimulation improves the odontoblastic differentiation of human dental pulp cells. Mol Med Rep 11: 3547-3552, 2015.
APA
Huang, Y., Jiang, H., Gong, Q., Li, X., & Ling, J. (2015). Lipopolysaccharide stimulation improves the odontoblastic differentiation of human dental pulp cells. Molecular Medicine Reports, 11, 3547-3552. https://doi.org/10.3892/mmr.2014.3120
MLA
Huang, Y., Jiang, H., Gong, Q., Li, X., Ling, J."Lipopolysaccharide stimulation improves the odontoblastic differentiation of human dental pulp cells". Molecular Medicine Reports 11.5 (2015): 3547-3552.
Chicago
Huang, Y., Jiang, H., Gong, Q., Li, X., Ling, J."Lipopolysaccharide stimulation improves the odontoblastic differentiation of human dental pulp cells". Molecular Medicine Reports 11, no. 5 (2015): 3547-3552. https://doi.org/10.3892/mmr.2014.3120
Copy and paste a formatted citation
x
Spandidos Publications style
Huang Y, Jiang H, Gong Q, Li X and Ling J: Lipopolysaccharide stimulation improves the odontoblastic differentiation of human dental pulp cells. Mol Med Rep 11: 3547-3552, 2015.
APA
Huang, Y., Jiang, H., Gong, Q., Li, X., & Ling, J. (2015). Lipopolysaccharide stimulation improves the odontoblastic differentiation of human dental pulp cells. Molecular Medicine Reports, 11, 3547-3552. https://doi.org/10.3892/mmr.2014.3120
MLA
Huang, Y., Jiang, H., Gong, Q., Li, X., Ling, J."Lipopolysaccharide stimulation improves the odontoblastic differentiation of human dental pulp cells". Molecular Medicine Reports 11.5 (2015): 3547-3552.
Chicago
Huang, Y., Jiang, H., Gong, Q., Li, X., Ling, J."Lipopolysaccharide stimulation improves the odontoblastic differentiation of human dental pulp cells". Molecular Medicine Reports 11, no. 5 (2015): 3547-3552. https://doi.org/10.3892/mmr.2014.3120
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team