Expression of cluster of differentiation-95 and relevant signaling molecules in liver cancer

Wang,Xuming; Wei,Kanglai; Zhang,Qiongguang; Zeng,Sien; Lin,Jing; Qiao,Li; Liu,Lijiang

doi:10.3892/mmr.2014.3129

Expression of cluster of differentiation-95 and relevant signaling molecules in liver cancer

Authors:
- Xuming Wang
- Kanglai Wei
- Qiongguang Zhang
- Sien Zeng
- Jing Lin
- Li Qiao
- Lijiang Liu
View Affiliations

Affiliations: Department of Pathology, Guilin Medical University, Guilin 541001, P.R. China, Department of Pathology, The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi 530021, P.R. China, State Key Laboratory of Virology, National Laboratory of Antiviral and Tumor of Traditional Chinese Medicine, Institute of Medical Virology, Research Center of Food and Drug Evaluation, School of Medicine, Wuhan University, Wuhan, Hubei 430056, P.R. China, Office of Graduate Student Affairs, Guilin Medical University, Guilin 541001, P.R. China, Department of Pathology and Pathophysiology, School of Medicine, Jianghan University, Wuhan Economy and Technology Development Zone, Wuhan, Hubei 430056, P.R. China
Published online on: December 22, 2014 https://doi.org/10.3892/mmr.2014.3129
Pages: 3375-3381
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

The present study investigated the protein expression levels of cluster of differentiation (CD)95, caspase‑8, caspase‑3 and poly(ADP‑ribose) polymerase 1 (PARP1) in liver cancer and its association with clinical pathological parameters. The results demonstrated that the expression of CD95 correlated with histological differentiation, liver cirrhosis, lymph node metastasis and distant metastasis (P<0.05), however, no correlations with gender, age, quantity of tumor nodules or T stage were observed (P>0.05). The expression of CD95 was upregulated using a plasmid, which led to an increase in the expression levels of caspase‑8 and caspase‑3 and a decrease in the expression of PARP1. Upregulation of CD95 also promoted the apoptosis of the liver cancer cells. These results indicated that CD95 was associated with liver cancer and promoted the apoptosis of liver cancer cells by caspase‑8, caspase‑3 and PARP1.

View Figures

View References

1	Yonehara S, Ishii A and Yonehara M: A cell-killing monoclonal antibody (anti-Fas) to a cell surface antigen co-downregulated with the receptor of tumor necrosis factor. J Exp Med. 169:1747–1756. 1989. View Article : Google Scholar : PubMed/NCBI
2	Trauth BC, Klas C, Peters AM, et al: Monoclonal antibody-mediated tumor regression by induction of apoptosis. Science. 245:301–305. 1989. View Article : Google Scholar : PubMed/NCBI
3	Itoh N, Yonehara S, Ishii A, et al: The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis. Cell. 66:233–243. 1991. View Article : Google Scholar : PubMed/NCBI
4	Oehm A, Behrmann I, Falk W, et al: Purification and molecular cloning of the APO-1 cell surface antigen, a member of the tumor necrosis factor/nerve growth factor receptor superfamily. Sequence identity with the Fas antigen. J Biol Chem. 267:10709–10715. 1992.PubMed/NCBI
5	Hammam O, Mahmoud O, Zahran M, et al: The role of fas/fas ligand system in the pathogenesis of liver cirrhosis and hepatocellular carcinoma. Hepat Mon. 12:e61322012. View Article : Google Scholar
6	El Bassiouny AE, El-Bassiouni NE, Nosseir MM, et al: Circulating and hepatic Fas expression in HCV-induced chronic liver disease and hepatocellular carcinoma. Medscape J Med. 10:1302008.PubMed/NCBI
7	Lee YB, Kyung Kim E, Park HJ, et al: Expression of Fas and Fas ligand in primary cutaneous squamous cell carcinoma in association with grade of tumor differentiation. Int J Dermatol. 52:1092–1097. 2013. View Article : Google Scholar
8	Sjostrom-Mattson J, Von Boguslawski K, Bengtsson NO, Mjaaland I, Salmenkivi K and Blomqvist C: The expression of p53, bcl-2, bax, fas and fasL in the primary tumor and lymph node metastases of breast cancer. Acta Oncol. 48:1137–1143. 2009. View Article : Google Scholar
9	Bebenek M, Dus D and Kozlak J: Fas expression in primary breast cancer is related to neoplastic infiltration of perilymphatic fat. Adv Med Sci. 53:49–53. 2008. View Article : Google Scholar : PubMed/NCBI
10	Yang D, Torres CM, Bardhan K, Zimmerman M, McGaha TL and Liu K: Decitabine and vorinostat cooperate to sensitize colon carcinoma cells to Fas ligand-induced apoptosis in vitro and tumor suppression in vivo. J Immunol. 188:4441–4449. 2012. View Article : Google Scholar : PubMed/NCBI
11	Yu Y, Bae S, Kim H, et al: The anti-tumor activity of vitamin C via the increase of Fas (CD95) and MHC I expression on human stomach cancer cell line, SNU1. Immune Netw. 11:210–215. 2011. View Article : Google Scholar : PubMed/NCBI
12	Volkmann M, Schiff JH, Hajjar Y, et al: Loss of CD95 expression is linked to most but not all p53 mutants in European hepatocellular carcinoma. J Mol Med (Berl). 79:594–600. 2001. View Article : Google Scholar
13	Hughes MA, Harper N, Butterworth M, Cain K, Cohen GM and MacFarlane M: Reconstitution of the death-inducing signaling complex reveals a substrate switch that determines CD95-mediated death or survival. Mol Cell. 35:265–279. 2009. View Article : Google Scholar : PubMed/NCBI
14	Kober AM, Legewie S, Pforr C, et al: Caspase-8 activity has an essential role in CD95/Fas-mediated MAPK activation. Cell Death Dis. 2:e2122011. View Article : Google Scholar : PubMed/NCBI
15	Bajt ML, Vonderfecht SL and Jaeschke H: Differential protection with inhibitors of caspase-8 and caspase-3 in murine models of tumor necrosis factor and Fas receptor-mediated hepatocellular apoptosis. Toxicol Appl Pharmacol. 175:243–252. 2001. View Article : Google Scholar : PubMed/NCBI
16	Xiao LJ, Zhao S, Zhao EH, et al: Clinicopathological and prognostic significance of Ki-67, caspase-3 and p53 expression in gastric carcinomas. Oncol Lett. 6:1277–1284. 2013.PubMed/NCBI
17	Sun BH, Zhang J, Wang BJ, et al: Analysis of in vivo patterns of caspase 3 gene expression in primary hepatocellular carcinoma and its relationship to p21(WAF1) expression and hepatic apoptosis. World J Gastroenterol. 6:356–360. 2000.
18	Lindahl T, Satoh MS, Poirier GG and Klungland A: Post-translational modification of poly(ADP-ribose) polymerase induced by DNA strand breaks. Trends Biochem Sci. 20:405–411. 1995. View Article : Google Scholar : PubMed/NCBI
19	Bi FF, Li D and Yang Q: Hypomethylation of ETS transcription factor binding sites and upregulation of PARP1 expression in endometrial cancer. Biomed Res Int. 2013:9462682013. View Article : Google Scholar : PubMed/NCBI
20	Wu W, Kong Z, Duan X, et al: Inhibition of PARP1 by small interfering RNA enhances docetaxel activity against human prostate cancer PC3 cells. Biochem Biophys Res Commun. 442:127–132. 2013. View Article : Google Scholar : PubMed/NCBI
21	He W, Liu T, Shan Y, Zhu K and Li Y: PARP1 polymorphisms increase the risk of gastric cancer in a Chinese population. Mol Diagn Ther. 16:35–42. 2012. View Article : Google Scholar : PubMed/NCBI
22	Kim J, Pyun JA, Cho SW, Lee K and Kwack K: Lymph node metastasis of gastric cancer is associated with the interaction between poly (ADP-ribose) polymerase 1 and matrix metallopeptidase 2. DNA Cell Biol. 30:1011–1017. 2011. View Article : Google Scholar : PubMed/NCBI