Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
May-2015 Volume 11 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
May-2015 Volume 11 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Taxol stabilizes gap junctions and reduces ischemic ventricular arrhythmias in rats in vivo

  • Authors:
    • Deguo Wang
    • Wen Xing
    • Xin Wang
    • Hongjun Zhu
  • View Affiliations / Copyright

    Affiliations: Department of Gerontology, Yijishan Hospital of Wannan Medical College, Wuhu, Anhui 241001, P.R. China, Department of Cardiology, Anhui Provincial Hospital, Hefei, Anhui 231000, P.R. China
  • Pages: 3243-3248
    |
    Published online on: December 24, 2014
       https://doi.org/10.3892/mmr.2014.3137
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The dynamic movements of connexin 43 (Cx43) are regulated by microtubules and their associated proteins. Dysfunction of Cx43 in the ischemic myocardium is correlated with ventricular arrhythmias (VAs). The present study aimed to determine the effects of microtubules on Cx43 expression and distribution in myocardial tissue, as well as to examine the susceptibility of the heart to VAs during acute myocardial ischemia and reperfusion. Rats were subject to left coronary artery occlusion for 20 min followed by 20 min reperfusion and received taxol at different concentrations (0.1, 0.3 and 0.9 µmol·kg‑1 in 0.5 ml saline) intraperitoneally. Monophasic action potentials at the epicardium were recorded and analyzed using an electrocardiogram. Immunoblots and immunofluorescence staining were used to detect tubulin polymerization and Cx43 expression and distribution. Taxol pretreatment significantly ameliorated the depolymerization of microtubules, improved Cx43 expression and redistribution, reduced the occurrence of VAs, ameliorated shortening of 90% repolarization action potential durations (APD)90 and improved APD dispersion during myocardial ischemia‑reperfusion. The present study demonstrated that taxol reduced ischemic VAs and its mechanism may be correlated with the preservation of Cx43 by stabilizing microtubules.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Kieken F, Mutsaers N, Dolmatova E, et al: Structural and molecular mechanisms of gap junction remodeling in epicardial border zone myocytes following myocardial infarction. Circ Res. 104:1103–1112. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Shaw RM, Fay AJ, Puthenveedu MA, von Zastrow M, Jan YN and Jan LY: Microtubule plus-end-tracking proteins target gap junctions directly from the cell interior to adherens junctions. Cell. 128:547–560. 2007. View Article : Google Scholar : PubMed/NCBI

3 

Smyth JW, Hong TT, Gao D, et al: Limited forward trafficking of connexin 43 reduces cell-cell coupling in stressed human and mouse myocardium. J Clin Invest. 120:266–279. 2010. View Article : Google Scholar :

4 

Vandroux D, Schaeffer C, Tissier C, et al: Microtubule alteration is an early cellular reaction to the metabolic challenge in ischemic cardiomyocytes. Mol Cell Biochem. 258:99–108. 2004. View Article : Google Scholar : PubMed/NCBI

5 

Devillard L, Vandroux D, Tissier C, et al: Involvement of microtubules in the tolerance of cardiomyocytes to cold ischemia-reperfusion. Mol Cell Biochem. 307:149–157. 2008. View Article : Google Scholar

6 

Teng M, Dang YM, Zhang JP, et al: Microtubular stability affects cardiomyocyte glycolysis by HIF-1alpha expression and endonuclear aggregation during early stages of hypoxia. Am J Physiol Heart Circ Physiol. 298:H1919–H1931. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Howarth FC, Calaghan SC, Boyett MR and White E: Effect of the microtubule polymerizing agent taxol on contraction, Ca2+ transient and L-type Ca2+ current in rat ventricular myocytes. J Physiol. 516(Pt 2): 409–419. 1999. View Article : Google Scholar

8 

Xiao J, Zhao H, Liang D, et al: Taxol, a microtubule stabilizer, improves cardiac contractile function during ischemia in vitro. Pharmacology. 85:301–310. 2010. View Article : Google Scholar : PubMed/NCBI

9 

Xiao J, Cao H, Liang D, et al: Taxol, a microtubule stabilizer, prevents ischemic ventricular arrhythmias in rats. J Cell Mol Med. 15:1166–1176. 2011. View Article : Google Scholar

10 

Franz MR: Current status of monophasic action potential recording: theories, measurements and interpretations. Cardiovasc Res. 41:25–40. 1999. View Article : Google Scholar : PubMed/NCBI

11 

Walker MJ, Curtis MJ, Hearse DJ, et al: The Lambeth Conventions: guidelines for the study of arrhythmias in ischaemia infarction, and reperfusion. Cardiovasc Res. 22:447–455. 1988. View Article : Google Scholar : PubMed/NCBI

12 

Kawakami K, Chiba T, Katagiri N, et al: Paclitaxel increases high voltage-dependent calcium channel current in dorsal root ganglion neurons of the rat. J Pharmacol Sci. 120:187–195. 2012. View Article : Google Scholar : PubMed/NCBI

13 

De Vuyst E, Boengler K, Antoons G, Sipido KR, Schulz R and Leybaert L: Pharmacological modulation of connexin-formed channels in cardiac pathophysiology. Br J Pharmacol. 163:469–483. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Calaghan SC, Le Guennec JY and White E: Cytoskeletal modulation of electrical and mechanical activity in cardiac myocytes. Prog Biophys Mol Biol. 84:29–59. 2004. View Article : Google Scholar

15 

Tsuburaya R, Yasuda S, Ito Y, et al: Eicosapentaenoic acid reduces ischemic ventricular fibrillation via altering monophasic action potential in pigs. J Mol Cell Cardiol. 51:329–336. 2011. View Article : Google Scholar : PubMed/NCBI

16 

Chen Y, Zhang Q, Liao YH, et al: Effect of tumor necrosis factor-alpha on neutralization of ventricular fibrillation in rats with acute myocardial infarction. Mediators Inflamm. 2011:5652382011. View Article : Google Scholar

17 

Guo X, Gao X, Wang Y, Peng L, Zhu Y and Wang S: IKs protects from ventricular arrhythmia during cardiac ischemia and reperfusion in rabbits by preserving the repolarization reserve. PLoS One. 7:e315452012. View Article : Google Scholar : PubMed/NCBI

18 

Hori M, Sato H, Kitakaze M, et al: Beta-adrenergic stimulation disassembles microtubules in neonatal rat cultured cardiomyocytes through intracellular Ca2+ overload. Circ Res. 75:324–334. 1994. View Article : Google Scholar : PubMed/NCBI

19 

Saetersdal T, Greve G and Dalen H: Associations between beta-tubulin and mitochondria in adult isolated heart myocytes as shown by immunofluorescence and immunoelectron microscopy. Histochemistry. 95:1–10. 1990. View Article : Google Scholar : PubMed/NCBI

20 

Devillard L, Vandroux D, Tissier C, et al: Tubulin ligands suggest a microtubule-NADPH oxidase relationship in postischemic cardiomyocytes. Eur J Pharmacol. 548:64–73. 2006. View Article : Google Scholar : PubMed/NCBI

21 

Beardslee MA, Lerner DL, Tadros PN, et al: Dephosphorylation and intracellular redistribution of ventricular connexin43 during electrical uncoupling induced by ischemia. Circ Res. 87:656–662. 2000. View Article : Google Scholar : PubMed/NCBI

22 

Smyth JW, Hong TT, Gao D, et al: Limited forward trafficking of connexin 43 reduces cell-cell coupling in stressed human and mouse myocardium. J Clin Invest. 120:266–279. 2010. View Article : Google Scholar :

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wang D, Xing W, Wang X and Zhu H: Taxol stabilizes gap junctions and reduces ischemic ventricular arrhythmias in rats in vivo. Mol Med Rep 11: 3243-3248, 2015.
APA
Wang, D., Xing, W., Wang, X., & Zhu, H. (2015). Taxol stabilizes gap junctions and reduces ischemic ventricular arrhythmias in rats in vivo. Molecular Medicine Reports, 11, 3243-3248. https://doi.org/10.3892/mmr.2014.3137
MLA
Wang, D., Xing, W., Wang, X., Zhu, H."Taxol stabilizes gap junctions and reduces ischemic ventricular arrhythmias in rats in vivo". Molecular Medicine Reports 11.5 (2015): 3243-3248.
Chicago
Wang, D., Xing, W., Wang, X., Zhu, H."Taxol stabilizes gap junctions and reduces ischemic ventricular arrhythmias in rats in vivo". Molecular Medicine Reports 11, no. 5 (2015): 3243-3248. https://doi.org/10.3892/mmr.2014.3137
Copy and paste a formatted citation
x
Spandidos Publications style
Wang D, Xing W, Wang X and Zhu H: Taxol stabilizes gap junctions and reduces ischemic ventricular arrhythmias in rats in vivo. Mol Med Rep 11: 3243-3248, 2015.
APA
Wang, D., Xing, W., Wang, X., & Zhu, H. (2015). Taxol stabilizes gap junctions and reduces ischemic ventricular arrhythmias in rats in vivo. Molecular Medicine Reports, 11, 3243-3248. https://doi.org/10.3892/mmr.2014.3137
MLA
Wang, D., Xing, W., Wang, X., Zhu, H."Taxol stabilizes gap junctions and reduces ischemic ventricular arrhythmias in rats in vivo". Molecular Medicine Reports 11.5 (2015): 3243-3248.
Chicago
Wang, D., Xing, W., Wang, X., Zhu, H."Taxol stabilizes gap junctions and reduces ischemic ventricular arrhythmias in rats in vivo". Molecular Medicine Reports 11, no. 5 (2015): 3243-3248. https://doi.org/10.3892/mmr.2014.3137
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team