Interleukin‑1β increases permeability and upregulates the expression of vascular endothelial‑cadherin in human renal glomerular endothelial cells

Du,Linna; Dong,Fengyun; Guo,Ling; Hou,Yinglong; Yi,Fan; Liu,Ju; Xu,Dongmei

doi:10.3892/mmr.2015.3172

Interleukin‑1β increases permeability and upregulates the expression of vascular endothelial‑cadherin in human renal glomerular endothelial cells

Authors:
- Linna Du
- Fengyun Dong
- Ling Guo
- Yinglong Hou
- Fan Yi
- Ju Liu
- Dongmei Xu
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Affiliations: Department of Nephrology, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong 250014, P.R. China, Laboratory of Microvascular Medicine, Medical Research Center, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong 250014, P.R. China, Department of Cardiology, Shandong Provincial Qianfoshan Hospital, Shandong University, Jinan, Shandong 250014, P.R. China, Department of Pharmacology, Shandong University School of Medicine, Jinan, Shandong 250012, P.R. China
Published online on: January 9, 2015 https://doi.org/10.3892/mmr.2015.3172
Pages: 3708-3714

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Abstract

The renal glomerular capillary endothelium is part of the glomerular filtration barrier and is involved in acute and chronic inflammation of the glomerulus. Glomerular endothelial cells are a unique type of microvascular cell, which remain to be fully characterized. The aim of the present study was to examine the permeability of glomerular endothelial cells and their responses to interleukin (IL)‑1β, a pro‑inflammatory cytokine. Human glomerular endothelial cell (HRGEC) and human umbilical vein endothelial cell (HUVEC) monolayers were examined using a Transwell permeability assay, transendothelial electrical resistance (TEER) and by determining the expression of the adhesion molecule, vascular endothelial (VE)‑cadherin, in the absence or presence of 10 ng/ml IL‑1β. Compared with the HUVECs, the HRGECs demonstrated higher permeability, lower TEER and reduced expression of VE‑cadherin. IL‑1β induced an increase in the permeability and a decrease in the TEER of the HRGECs, however, to a lesser extent compared with the HUVECs. Following IL‑1β treatment, the expression of VE‑cadherin was increased in the HRGECs and decreased in the HUVECs. These results suggested that HRGECs have distinct biological properties and specific gene expression features in response to IL‑1β.

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