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Article

Lentivirus‑mediated silencing of spindle and kinetochore‑associated protein 1 inhibits the proliferation and invasion of neuronal glioblastoma cells

  • Authors:
    • Xiujuan Shi
    • Xianzhen Chen
    • Hu Peng
    • E Song
    • Ting Zhang
    • Junxiang Zhang
    • Jing Li
    • Himaya Swa
    • Yongxin Li
    • Sekwon Kim
    • Xiaoqing Liu
    • Chen Zhang
  • View Affiliations / Copyright

    Affiliations: Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, P.R. China, Lixiang Eye Hospital of Soochow University, Suzhou, Jiangsu 242000, P.R. China, Medical Technology College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, P.R. China, Xuancheng Central Hospital, Xuancheng, Anhui 242000, P.R. China, Tongji Hospital Affiliated to Tongji University, School of Medicine, Tongji University, Shanghai 200065, P.R. China, Specialized Graduate School Science and Technology Convergence, Department of Marine Bio Convergence Science, Pukyong National University, Busan 608‑737, Republic of Korea
  • Pages: 3533-3538
    |
    Published online on: January 9, 2015
       https://doi.org/10.3892/mmr.2015.3175
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Abstract

Spindle and kinetochore‑associated protein 1 (SKA1) is an important component of the human kinetochore, which plays a key role in mitosis. The resent study was designed to investigate the role of SKA1 in human glioblastoma. The results of the present study demonstrated that SKA1 was expressed in human glioblastoma cells. In addition, the knockdown of SKA1 expression in the A172 and U251 human glioblastoma cell lines was accomplished using a lentivirus infection method. An MTT assay demonstrated that downregulation of SKA1 may inhibit cell proliferation, without affecting the cell cycle. Furthermore, knockdown of SKA1 expression resulted in reduced cell invasion. The results of the present study indicated that SKA1 may be a potential target protein for antiproliferative and anti‑invasive therapeutic strategies of human glioblastoma.
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Copy and paste a formatted citation
Spandidos Publications style
Shi X, Chen X, Peng H, Song E, Zhang T, Zhang J, Li J, Swa H, Li Y, Kim S, Kim S, et al: Lentivirus‑mediated silencing of spindle and kinetochore‑associated protein 1 inhibits the proliferation and invasion of neuronal glioblastoma cells. Mol Med Rep 11: 3533-3538, 2015.
APA
Shi, X., Chen, X., Peng, H., Song, E., Zhang, T., Zhang, J. ... Zhang, C. (2015). Lentivirus‑mediated silencing of spindle and kinetochore‑associated protein 1 inhibits the proliferation and invasion of neuronal glioblastoma cells. Molecular Medicine Reports, 11, 3533-3538. https://doi.org/10.3892/mmr.2015.3175
MLA
Shi, X., Chen, X., Peng, H., Song, E., Zhang, T., Zhang, J., Li, J., Swa, H., Li, Y., Kim, S., Liu, X., Zhang, C."Lentivirus‑mediated silencing of spindle and kinetochore‑associated protein 1 inhibits the proliferation and invasion of neuronal glioblastoma cells". Molecular Medicine Reports 11.5 (2015): 3533-3538.
Chicago
Shi, X., Chen, X., Peng, H., Song, E., Zhang, T., Zhang, J., Li, J., Swa, H., Li, Y., Kim, S., Liu, X., Zhang, C."Lentivirus‑mediated silencing of spindle and kinetochore‑associated protein 1 inhibits the proliferation and invasion of neuronal glioblastoma cells". Molecular Medicine Reports 11, no. 5 (2015): 3533-3538. https://doi.org/10.3892/mmr.2015.3175
Copy and paste a formatted citation
x
Spandidos Publications style
Shi X, Chen X, Peng H, Song E, Zhang T, Zhang J, Li J, Swa H, Li Y, Kim S, Kim S, et al: Lentivirus‑mediated silencing of spindle and kinetochore‑associated protein 1 inhibits the proliferation and invasion of neuronal glioblastoma cells. Mol Med Rep 11: 3533-3538, 2015.
APA
Shi, X., Chen, X., Peng, H., Song, E., Zhang, T., Zhang, J. ... Zhang, C. (2015). Lentivirus‑mediated silencing of spindle and kinetochore‑associated protein 1 inhibits the proliferation and invasion of neuronal glioblastoma cells. Molecular Medicine Reports, 11, 3533-3538. https://doi.org/10.3892/mmr.2015.3175
MLA
Shi, X., Chen, X., Peng, H., Song, E., Zhang, T., Zhang, J., Li, J., Swa, H., Li, Y., Kim, S., Liu, X., Zhang, C."Lentivirus‑mediated silencing of spindle and kinetochore‑associated protein 1 inhibits the proliferation and invasion of neuronal glioblastoma cells". Molecular Medicine Reports 11.5 (2015): 3533-3538.
Chicago
Shi, X., Chen, X., Peng, H., Song, E., Zhang, T., Zhang, J., Li, J., Swa, H., Li, Y., Kim, S., Liu, X., Zhang, C."Lentivirus‑mediated silencing of spindle and kinetochore‑associated protein 1 inhibits the proliferation and invasion of neuronal glioblastoma cells". Molecular Medicine Reports 11, no. 5 (2015): 3533-3538. https://doi.org/10.3892/mmr.2015.3175
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