Lentivirus‑mediated silencing of spindle and kinetochore‑associated protein 1 inhibits the proliferation and invasion of neuronal glioblastoma cells

Shi,Xiujuan; Chen,Xianzhen; Peng,Hu; Song,E; Zhang,Ting; Zhang,Junxiang; Li,Jing; Swa,Himaya; Li,Yongxin; Kim,Sekwon; Liu,Xiaoqing; Zhang,Chen

doi:10.3892/mmr.2015.3175

Lentivirus‑mediated silencing of spindle and kinetochore‑associated protein 1 inhibits the proliferation and invasion of neuronal glioblastoma cells

Authors:
- Xiujuan Shi
- Xianzhen Chen
- Hu Peng
- E Song
- Ting Zhang
- Junxiang Zhang
- Jing Li
- Himaya Swa
- Yongxin Li
- Sekwon Kim
- Xiaoqing Liu
- Chen Zhang
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Affiliations: Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai 200072, P.R. China, Lixiang Eye Hospital of Soochow University, Suzhou, Jiangsu 242000, P.R. China, Medical Technology College, Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310053, P.R. China, Xuancheng Central Hospital, Xuancheng, Anhui 242000, P.R. China, Tongji Hospital Affiliated to Tongji University, School of Medicine, Tongji University, Shanghai 200065, P.R. China, Specialized Graduate School Science and Technology Convergence, Department of Marine Bio Convergence Science, Pukyong National University, Busan 608‑737, Republic of Korea
Published online on: January 9, 2015 https://doi.org/10.3892/mmr.2015.3175
Pages: 3533-3538

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Abstract

Spindle and kinetochore‑associated protein 1 (SKA1) is an important component of the human kinetochore, which plays a key role in mitosis. The resent study was designed to investigate the role of SKA1 in human glioblastoma. The results of the present study demonstrated that SKA1 was expressed in human glioblastoma cells. In addition, the knockdown of SKA1 expression in the A172 and U251 human glioblastoma cell lines was accomplished using a lentivirus infection method. An MTT assay demonstrated that downregulation of SKA1 may inhibit cell proliferation, without affecting the cell cycle. Furthermore, knockdown of SKA1 expression resulted in reduced cell invasion. The results of the present study indicated that SKA1 may be a potential target protein for antiproliferative and anti‑invasive therapeutic strategies of human glioblastoma.

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