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Article

Ampelopsis japonica ethanol extract suppresses migration and invasion in human MDA‑MB‑231 breast cancer cells

Corrigendum in: /10.3892/mmr.2025.13527
  • Authors:
    • Kyoung Jin Nho
    • Jin Mi Chun
    • Dong‑Seon Kim
    • Ho Kyoung Kim
  • View Affiliations / Copyright

    Affiliations: Herbal Medicine Resources Group, Korea Institute of Oriental Medicine, Yuseong‑gu, Daejeon 305‑811, Republic of Korea
  • Pages: 3722-3728
    |
    Published online on: January 9, 2015
       https://doi.org/10.3892/mmr.2015.3179
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Abstract

Ampelopsis japonica (AJ) is a well‑known traditional oriental herb with anti‑inflammatory and anticancer activities. However, the molecular mechanisms by which AJ inhibits metastasis in breast cancer cells remain to be elucidated. The aim of the present study was to investigate the effects of AJ ethanol extract (EAJ) on highly metastatic human MDA‑MB‑231 breast cancer cells in vitro. AJ was extracted and chemically characterized. Cell proliferation was determined using a CCK‑8 assay and migration was detected using a wound healing motility assay. A Transwell assay was used to evaluate the invasion and metastatic capabilities of the MDA‑MB‑231 cells. In addition, the mRNA expression levels of metalloproteinase (MMP)‑2 and MMP‑9 and tissue inhibitors of metalloproteinases (TIMP)‑1 and TIMP‑2 were evaluated using reverse transcription quantitative polymerase chain reaction in vitro. The results of the present study characterized the signaling cascades that mediated the antimetastatic activity of AJ in the human MDA‑MB‑231 breast cancer cell line. EAJ significantly suppressed the migration and invasion of MDA‑MB‑231 cells in vitro and inhibited the expression of metalloproteinase (MMP)‑2 and MMP‑9. These findings identified the biological activity of EAJ in an in vitro model of cancer metastasis and provided a rationale for further investigation.
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Copy and paste a formatted citation
Spandidos Publications style
Nho KJ, Chun JM, Kim DS and Kim HK: Ampelopsis japonica ethanol extract suppresses migration and invasion in human MDA‑MB‑231 breast cancer cells Corrigendum in /10.3892/mmr.2025.13527. Mol Med Rep 11: 3722-3728, 2015.
APA
Nho, K.J., Chun, J.M., Kim, D., & Kim, H.K. (2015). Ampelopsis japonica ethanol extract suppresses migration and invasion in human MDA‑MB‑231 breast cancer cells Corrigendum in /10.3892/mmr.2025.13527. Molecular Medicine Reports, 11, 3722-3728. https://doi.org/10.3892/mmr.2015.3179
MLA
Nho, K. J., Chun, J. M., Kim, D., Kim, H. K."Ampelopsis japonica ethanol extract suppresses migration and invasion in human MDA‑MB‑231 breast cancer cells Corrigendum in /10.3892/mmr.2025.13527". Molecular Medicine Reports 11.5 (2015): 3722-3728.
Chicago
Nho, K. J., Chun, J. M., Kim, D., Kim, H. K."Ampelopsis japonica ethanol extract suppresses migration and invasion in human MDA‑MB‑231 breast cancer cells Corrigendum in /10.3892/mmr.2025.13527". Molecular Medicine Reports 11, no. 5 (2015): 3722-3728. https://doi.org/10.3892/mmr.2015.3179
Copy and paste a formatted citation
x
Spandidos Publications style
Nho KJ, Chun JM, Kim DS and Kim HK: Ampelopsis japonica ethanol extract suppresses migration and invasion in human MDA‑MB‑231 breast cancer cells Corrigendum in /10.3892/mmr.2025.13527. Mol Med Rep 11: 3722-3728, 2015.
APA
Nho, K.J., Chun, J.M., Kim, D., & Kim, H.K. (2015). Ampelopsis japonica ethanol extract suppresses migration and invasion in human MDA‑MB‑231 breast cancer cells Corrigendum in /10.3892/mmr.2025.13527. Molecular Medicine Reports, 11, 3722-3728. https://doi.org/10.3892/mmr.2015.3179
MLA
Nho, K. J., Chun, J. M., Kim, D., Kim, H. K."Ampelopsis japonica ethanol extract suppresses migration and invasion in human MDA‑MB‑231 breast cancer cells Corrigendum in /10.3892/mmr.2025.13527". Molecular Medicine Reports 11.5 (2015): 3722-3728.
Chicago
Nho, K. J., Chun, J. M., Kim, D., Kim, H. K."Ampelopsis japonica ethanol extract suppresses migration and invasion in human MDA‑MB‑231 breast cancer cells Corrigendum in /10.3892/mmr.2025.13527". Molecular Medicine Reports 11, no. 5 (2015): 3722-3728. https://doi.org/10.3892/mmr.2015.3179
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