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Article

Overexpression of isocitrate dehydrogenase-1R132H enhances the proliferation of A172 glioma cells via aerobic glycolysis

  • Authors:
    • Quanmin Nie
    • Pin Guo
    • Liemei Guo
    • Jin Lan
    • Yingying Lin
    • Fang Guo
    • Sunhai  Zhou
    • Jianwei Ge
    • Qing Mao
    • Xiaoxiong Li
    • Yongming Qiu
  • View Affiliations / Copyright

    Affiliations: Department of Neurosurgery, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai 200127, P.R. China, Shanghai Institute of Head Trauma, Shanghai 200127, P.R. China, Laboratory of Targeted Tumor Therapy, Key Laboratory of Systems Biology, Shanghai Advanced Research Institute, Chinese Academy of Sciences, Shanghai 201210, P.R. China
  • Pages: 3715-3721
    |
    Published online on: January 13, 2015
       https://doi.org/10.3892/mmr.2015.3187
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Abstract

Gliomas are the most common type of primary malignancy of the central nervous system. The identification of mutations in the gene encoding isocitrate dehydrogenase‑1 (IDH1) represents a key area of investigation in studies on glioma. The IDH1R132H mutation is a heterozygous point mutation, which affects the amino acid arginine at position 132, however, the metabolic importance of this mutation in tumor cell growth remains to be elucidated. In the present study, A172 glioma cell lines stably overexpressing either wild‑type IDH1 or IDH1R132H were produced. The results demonstrated that the IDH1R132H mutation enhanced the proliferation of the A172 glioma cells in vitro. Furthermore, IDH1R132H performed this function by elevating the expression levels of hypoxia inducible factor‑1α, leading to an increase in the expression levels of the key glycolytic enzymes, glucose transporter 1 and hexokinase 2. Therefore, the metabolism was shifted towards aerobic glycolysis, leading to an increase in glucose uptake and lactate production. These findings demonstrated that the IDH1R132H molecular target was involved in orchestrating the Warburg effect in mutant IDH1R132H glioma cells.
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Copy and paste a formatted citation
Spandidos Publications style
Nie Q, Guo P, Guo L, Lan J, Lin Y, Guo F, Zhou S, Ge J, Mao Q, Li X, Li X, et al: Overexpression of isocitrate dehydrogenase-1R132H enhances the proliferation of A172 glioma cells via aerobic glycolysis. Mol Med Rep 11: 3715-3721, 2015.
APA
Nie, Q., Guo, P., Guo, L., Lan, J., Lin, Y., Guo, F. ... Qiu, Y. (2015). Overexpression of isocitrate dehydrogenase-1R132H enhances the proliferation of A172 glioma cells via aerobic glycolysis. Molecular Medicine Reports, 11, 3715-3721. https://doi.org/10.3892/mmr.2015.3187
MLA
Nie, Q., Guo, P., Guo, L., Lan, J., Lin, Y., Guo, F., Zhou, S., Ge, J., Mao, Q., Li, X., Qiu, Y."Overexpression of isocitrate dehydrogenase-1R132H enhances the proliferation of A172 glioma cells via aerobic glycolysis". Molecular Medicine Reports 11.5 (2015): 3715-3721.
Chicago
Nie, Q., Guo, P., Guo, L., Lan, J., Lin, Y., Guo, F., Zhou, S., Ge, J., Mao, Q., Li, X., Qiu, Y."Overexpression of isocitrate dehydrogenase-1R132H enhances the proliferation of A172 glioma cells via aerobic glycolysis". Molecular Medicine Reports 11, no. 5 (2015): 3715-3721. https://doi.org/10.3892/mmr.2015.3187
Copy and paste a formatted citation
x
Spandidos Publications style
Nie Q, Guo P, Guo L, Lan J, Lin Y, Guo F, Zhou S, Ge J, Mao Q, Li X, Li X, et al: Overexpression of isocitrate dehydrogenase-1R132H enhances the proliferation of A172 glioma cells via aerobic glycolysis. Mol Med Rep 11: 3715-3721, 2015.
APA
Nie, Q., Guo, P., Guo, L., Lan, J., Lin, Y., Guo, F. ... Qiu, Y. (2015). Overexpression of isocitrate dehydrogenase-1R132H enhances the proliferation of A172 glioma cells via aerobic glycolysis. Molecular Medicine Reports, 11, 3715-3721. https://doi.org/10.3892/mmr.2015.3187
MLA
Nie, Q., Guo, P., Guo, L., Lan, J., Lin, Y., Guo, F., Zhou, S., Ge, J., Mao, Q., Li, X., Qiu, Y."Overexpression of isocitrate dehydrogenase-1R132H enhances the proliferation of A172 glioma cells via aerobic glycolysis". Molecular Medicine Reports 11.5 (2015): 3715-3721.
Chicago
Nie, Q., Guo, P., Guo, L., Lan, J., Lin, Y., Guo, F., Zhou, S., Ge, J., Mao, Q., Li, X., Qiu, Y."Overexpression of isocitrate dehydrogenase-1R132H enhances the proliferation of A172 glioma cells via aerobic glycolysis". Molecular Medicine Reports 11, no. 5 (2015): 3715-3721. https://doi.org/10.3892/mmr.2015.3187
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