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Article

Effect of orexin A on apoptosis in BGC-823 gastric cancer cells via OX1R through the AKT signaling pathway

  • Authors:
    • Jing Wen
    • Yuyan Zhao
    • Yang Shen
    • Lei Guo
  • View Affiliations / Copyright

    Affiliations: Department of Endocrinology, First Affiliated Hospital, China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Orthopedic Surgery, First Affiliated Hospital, China Medical University, Shenyang, Liaoning 110001, P.R. China
  • Pages: 3439-3444
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    Published online on: January 13, 2015
       https://doi.org/10.3892/mmr.2015.3190
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Abstract

Orexins are a class of peptides involved in the regulation of food intake, energy homeostasis, the sleep‑wake cycle and gastrointestinal function. Recent studies have demonstrated that orexin A may influence apoptosis and proliferation in numerous types of cancer cells. However, the effect of orexin A on gastric cancer cells and its mechanisms of action remain elusive. In the present study, BGC‑823 gastric cancer cells were treated with orexin A (10‑10‑10‑6 M) in vitro and the expression levels of orexin receptor 1 (OX1R) protein in cells was then determined. The proliferation, viability and apoptosis of BGC‑823 cells were detected. In addition, BGC‑823 cells were treated with AKT inhibitor PF‑04691502 or OX1R‑specific antagonist SB334867 in combination with orexin A, in order to examine the activation of AKT and caspase‑3. The results showed that orexin A (10‑10‑10‑6 M) stimulated the OX1R protein expression in BGC‑823 cells, which improved the proliferation and viability of the cells as well as protected them from apoptosis. Phosphorylated AKT protein was significantly increased in BGC‑823 cells following treatment with orexin A. Moreover, 10‑8 M orexin A reduced the proapoptotic activity of caspase‑3 (by ≤30%). The OX1R antagonist SB334867 (10‑6 M) and AKT antagonist PF‑04691502 (10‑6 M), when used individually or in combination, abolished the effect of orexin A (10‑8 M) on BGC-823 cells. In conclusion, the results of the present study demonstrated that orexin A inhibited gastric cancer cell apoptosis via OX1R through the AKT signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Wen J, Zhao Y, Shen Y and Guo L: Effect of orexin A on apoptosis in BGC-823 gastric cancer cells via OX1R through the AKT signaling pathway. Mol Med Rep 11: 3439-3444, 2015.
APA
Wen, J., Zhao, Y., Shen, Y., & Guo, L. (2015). Effect of orexin A on apoptosis in BGC-823 gastric cancer cells via OX1R through the AKT signaling pathway. Molecular Medicine Reports, 11, 3439-3444. https://doi.org/10.3892/mmr.2015.3190
MLA
Wen, J., Zhao, Y., Shen, Y., Guo, L."Effect of orexin A on apoptosis in BGC-823 gastric cancer cells via OX1R through the AKT signaling pathway". Molecular Medicine Reports 11.5 (2015): 3439-3444.
Chicago
Wen, J., Zhao, Y., Shen, Y., Guo, L."Effect of orexin A on apoptosis in BGC-823 gastric cancer cells via OX1R through the AKT signaling pathway". Molecular Medicine Reports 11, no. 5 (2015): 3439-3444. https://doi.org/10.3892/mmr.2015.3190
Copy and paste a formatted citation
x
Spandidos Publications style
Wen J, Zhao Y, Shen Y and Guo L: Effect of orexin A on apoptosis in BGC-823 gastric cancer cells via OX1R through the AKT signaling pathway. Mol Med Rep 11: 3439-3444, 2015.
APA
Wen, J., Zhao, Y., Shen, Y., & Guo, L. (2015). Effect of orexin A on apoptosis in BGC-823 gastric cancer cells via OX1R through the AKT signaling pathway. Molecular Medicine Reports, 11, 3439-3444. https://doi.org/10.3892/mmr.2015.3190
MLA
Wen, J., Zhao, Y., Shen, Y., Guo, L."Effect of orexin A on apoptosis in BGC-823 gastric cancer cells via OX1R through the AKT signaling pathway". Molecular Medicine Reports 11.5 (2015): 3439-3444.
Chicago
Wen, J., Zhao, Y., Shen, Y., Guo, L."Effect of orexin A on apoptosis in BGC-823 gastric cancer cells via OX1R through the AKT signaling pathway". Molecular Medicine Reports 11, no. 5 (2015): 3439-3444. https://doi.org/10.3892/mmr.2015.3190
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