Synergistic effect of all‑trans retinoic acid in combination with protein kinase C 412 in FMS-like tyrosine kinase 3-mutated acute myeloid leukemia cells

  • Authors:
    • Hoang Thanh Chi
    • Bui Thi Kim Ly
    • Hoang Anh Vu
    • Yuko Sato
    • Phu Chi Dung
    • Phan Thi Xinh
  • View Affiliations

  • Published online on: January 15, 2015     https://doi.org/10.3892/mmr.2015.3203
  • Pages: 3969-3975
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Acute myeloid leukemia (AML) is a heterogeneous disease. Numerous molecular abnormalities have been identified in AML and, amongst these, FMS‑like tyrosine kinase 3 (FLT3) mutations are one of the most common somatic alterations detected. In the present study, an in vitro investigation was performed to evaluate the effects of all‑trans retinoic acid (ATRA) and PKC412, alone and in combination, in FLT3‑mutated AML cell lines. Trypan blue exclusion test, as well as morphological, western blot and isobologram analyses were conducted. The results indicated that the combined ATRA and PKC412 treatment exhibited additive or synergistic effects in FLT3‑mutated AML cell lines. These results provided in vitro evidence for the future clinical trials evaluating the effects of a combination treatment using PKC412 and ATRA on AML patients with FLT3‑mutations.
View Figures
View References

Related Articles

Journal Cover

May-2015
Volume 11 Issue 5

Print ISSN: 1791-2997
Online ISSN:1791-3004

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Chi HT, Ly BT, Vu HA, Sato Y, Dung PC and Xinh PT: Synergistic effect of all‑trans retinoic acid in combination with protein kinase C 412 in FMS-like tyrosine kinase 3-mutated acute myeloid leukemia cells. Mol Med Rep 11: 3969-3975, 2015
APA
Chi, H.T., Ly, B.T., Vu, H.A., Sato, Y., Dung, P.C., & Xinh, P.T. (2015). Synergistic effect of all‑trans retinoic acid in combination with protein kinase C 412 in FMS-like tyrosine kinase 3-mutated acute myeloid leukemia cells. Molecular Medicine Reports, 11, 3969-3975. https://doi.org/10.3892/mmr.2015.3203
MLA
Chi, H. T., Ly, B. T., Vu, H. A., Sato, Y., Dung, P. C., Xinh, P. T."Synergistic effect of all‑trans retinoic acid in combination with protein kinase C 412 in FMS-like tyrosine kinase 3-mutated acute myeloid leukemia cells". Molecular Medicine Reports 11.5 (2015): 3969-3975.
Chicago
Chi, H. T., Ly, B. T., Vu, H. A., Sato, Y., Dung, P. C., Xinh, P. T."Synergistic effect of all‑trans retinoic acid in combination with protein kinase C 412 in FMS-like tyrosine kinase 3-mutated acute myeloid leukemia cells". Molecular Medicine Reports 11, no. 5 (2015): 3969-3975. https://doi.org/10.3892/mmr.2015.3203