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Article

Synergistic effect of all‑trans retinoic acid in combination with protein kinase C 412 in FMS-like tyrosine kinase 3-mutated acute myeloid leukemia cells

  • Authors:
    • Hoang Thanh Chi
    • Bui Thi Kim Ly
    • Hoang Anh Vu
    • Yuko Sato
    • Phu Chi Dung
    • Phan Thi Xinh
  • View Affiliations / Copyright

    Affiliations: Department of Molecular Cytogenetics, Blood Transfusion and Hematology Hospital Ho Chi Minh City, Ho Chi Minh City 711512, Vietnam, Department of Medical Genome Sciences, Graduate School of Frontier Sciences, The University of Tokyo, Tokyo 108‑8639, Japan, Center for Molecular Biomedicine, The University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City 748908, Vietnam, Japanese Red Cross College of Nursing, Tokyo 150‑0012, Japan, Department of First Pediatric Hematology, Blood Transfusion and Hematology Hospital Ho Chi Minh City, Ho Chi Minh City 711512, Vietnam
  • Pages: 3969-3975
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    Published online on: January 15, 2015
       https://doi.org/10.3892/mmr.2015.3203
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Abstract

Acute myeloid leukemia (AML) is a heterogeneous disease. Numerous molecular abnormalities have been identified in AML and, amongst these, FMS‑like tyrosine kinase 3 (FLT3) mutations are one of the most common somatic alterations detected. In the present study, an in vitro investigation was performed to evaluate the effects of all‑trans retinoic acid (ATRA) and PKC412, alone and in combination, in FLT3‑mutated AML cell lines. Trypan blue exclusion test, as well as morphological, western blot and isobologram analyses were conducted. The results indicated that the combined ATRA and PKC412 treatment exhibited additive or synergistic effects in FLT3‑mutated AML cell lines. These results provided in vitro evidence for the future clinical trials evaluating the effects of a combination treatment using PKC412 and ATRA on AML patients with FLT3‑mutations.
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Copy and paste a formatted citation
Spandidos Publications style
Chi HT, Ly BT, Vu HA, Sato Y, Dung PC and Xinh PT: Synergistic effect of all‑trans retinoic acid in combination with protein kinase C 412 in FMS-like tyrosine kinase 3-mutated acute myeloid leukemia cells. Mol Med Rep 11: 3969-3975, 2015.
APA
Chi, H.T., Ly, B.T., Vu, H.A., Sato, Y., Dung, P.C., & Xinh, P.T. (2015). Synergistic effect of all‑trans retinoic acid in combination with protein kinase C 412 in FMS-like tyrosine kinase 3-mutated acute myeloid leukemia cells. Molecular Medicine Reports, 11, 3969-3975. https://doi.org/10.3892/mmr.2015.3203
MLA
Chi, H. T., Ly, B. T., Vu, H. A., Sato, Y., Dung, P. C., Xinh, P. T."Synergistic effect of all‑trans retinoic acid in combination with protein kinase C 412 in FMS-like tyrosine kinase 3-mutated acute myeloid leukemia cells". Molecular Medicine Reports 11.5 (2015): 3969-3975.
Chicago
Chi, H. T., Ly, B. T., Vu, H. A., Sato, Y., Dung, P. C., Xinh, P. T."Synergistic effect of all‑trans retinoic acid in combination with protein kinase C 412 in FMS-like tyrosine kinase 3-mutated acute myeloid leukemia cells". Molecular Medicine Reports 11, no. 5 (2015): 3969-3975. https://doi.org/10.3892/mmr.2015.3203
Copy and paste a formatted citation
x
Spandidos Publications style
Chi HT, Ly BT, Vu HA, Sato Y, Dung PC and Xinh PT: Synergistic effect of all‑trans retinoic acid in combination with protein kinase C 412 in FMS-like tyrosine kinase 3-mutated acute myeloid leukemia cells. Mol Med Rep 11: 3969-3975, 2015.
APA
Chi, H.T., Ly, B.T., Vu, H.A., Sato, Y., Dung, P.C., & Xinh, P.T. (2015). Synergistic effect of all‑trans retinoic acid in combination with protein kinase C 412 in FMS-like tyrosine kinase 3-mutated acute myeloid leukemia cells. Molecular Medicine Reports, 11, 3969-3975. https://doi.org/10.3892/mmr.2015.3203
MLA
Chi, H. T., Ly, B. T., Vu, H. A., Sato, Y., Dung, P. C., Xinh, P. T."Synergistic effect of all‑trans retinoic acid in combination with protein kinase C 412 in FMS-like tyrosine kinase 3-mutated acute myeloid leukemia cells". Molecular Medicine Reports 11.5 (2015): 3969-3975.
Chicago
Chi, H. T., Ly, B. T., Vu, H. A., Sato, Y., Dung, P. C., Xinh, P. T."Synergistic effect of all‑trans retinoic acid in combination with protein kinase C 412 in FMS-like tyrosine kinase 3-mutated acute myeloid leukemia cells". Molecular Medicine Reports 11, no. 5 (2015): 3969-3975. https://doi.org/10.3892/mmr.2015.3203
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