Correlation of virtual touch tissue quantification and liver biopsy in a rat liver fibrosis model

Hu,Zhiwen; Luo,Jialun; Wei,Hongqin; Ou,Wencai; Xiao,Shuyi; Gan,Man; Ma,Suihong; He,Jingguang; Wu,Daihong; Feng,Guiying; Wei,Jinglu; Liu,Jianhua

doi:10.3892/mmr.2015.3209

May-2015 Volume 11 Issue 5

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May-2015 Volume 11 Issue 5

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Article

Correlation of virtual touch tissue quantification and liver biopsy in a rat liver fibrosis model

Authors:
- Zhiwen Hu
- Jialun Luo
- Hongqin Wei
- Wencai Ou
- Shuyi Xiao
- Man Gan
- Suihong Ma
- Jingguang He
- Daihong Wu
- Guiying Feng
- Jinglu Wei
- Jianhua Liu
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Affiliations: Medical School, Jinan University, Guangzhou, Guangdong 510632, P.R. China, College of Health Sciences, Guangzhou Medical University, Guangzhou, Guangdong 510180, P.R. China, Department of Medical Ultrasound, Guangzhou First People's Hospital, Guangzhou Medical University, Guangzhou, Guangdong 510180, P.R. China
Pages: 3694-3700
|
Published online on: January 16, 2015

https://doi.org/10.3892/mmr.2015.3209
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Abstract

Liver fibrosis assessment is very important to the treatment of chronic liver disease. In the present study, Virtual Touch Tissue Quantification (VTQ) and eSie Touch™ elasticity imaging techniques were used to examine the rat liver fibrosis model. Rat liver fibrosis was induced with thioacetamide and the degree of liver fibrosis was determined using pathological diagnosis as a gold standard. The right lobe of the liver was also examined with the VTQ and eSie Touch™ techniques. The VTQ and serological results were correlated and analyzed. The results were compared with those obtained from liver biopsies to investigate the accuracy and diagnostic value of eSie Touch™ and VTQ on the classification of liver fibrosis in rats. A total of 30 successful modeling cases were obtained, with a success rate of 86%. The mean acoustic radiation force impulse (ARFI) elastography‑VTQ values were 1.08, 1.51, 1.88 and 2.50 m/sec for the normal and F1/F2, F3 and F4 fibrosis groups, respectively. A significant correlation (r = 0.969) was identified between the ARFI measurements and the degree of fibrosis assessed by pathological examination (P<0.001). The histological staging results correlated with those of the eSie Touch™ elasticity imaging of the biopsy site (r = 0.913, P<0.001). The predictive values of ARFI for various stages of fibrosis were as follows: F≥1 and 2 ‑ cut‑off >1.250 m/sec (when Vs >1.250 m/sec, the pathological grading was ≥F1/F2) [Area under receiver operating characteristic (AUROC) = 1.00], F≥3 ‑ cut‑off >1.685 m/sec (when Vs >1.685 m/sec, the pathological grading was ≥F3; AUROC = 1.00) and F≥4 ‑ cut‑off >2.166 m/sec (when Vs >2.166 m/sec, the pathological grading is cirrhosis; AUROC = 1.00). In conclusion, the eSie Touch™ elasticity imaging and VTQ techniques may be successfully adopted to assess the extent of liver stiffness. These techniques are expected to replace liver biopsy.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Correlation of virtual touch tissue quantification and liver biopsy in a rat liver fibrosis model

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