Expression of tumor necrosis factor receptor-assicated factor 4 correlates with expression of Girdin and promotes nuclear translocation of Girdin in breast cancer

Wang,Ailian; Wang,Jian; Sun,Lili; Jin,Juan; Ren,Huayan; Yang,Fan; Diao,Kexin; Wei,Minjie; Mi,Xiaoyi

doi:10.3892/mmr.2015.3211

Expression of tumor necrosis factor receptor-assicated factor 4 correlates with expression of Girdin and promotes nuclear translocation of Girdin in breast cancer

Authors:
- Ailian Wang
- Jian Wang
- Lili Sun
- Juan Jin
- Huayan Ren
- Fan Yang
- Kexin Diao
- Minjie Wei
- Xiaoyi Mi
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Affiliations: Department of Pathology, First Affiliated Hospital and College of Basic Medical Sciences, China Medical University, Shenyang, Liaoning 110001, P.R. China, Department of Pathology, Shaanxi Baoji Centre Hospital, Baoji, Shaanxi 721008, P.R. China, Department of Pharmacology, Pharmaceutical College, China Medical University, Shenyang, Liaoning 110001, P.R. China
Published online on: January 16, 2015 https://doi.org/10.3892/mmr.2015.3211
Pages: 3635-3641

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Abstract

Overexpression of tumor necrosis factor receptor‑associated factor 4 (TRAF4) has been reported in several human malignancies; however its association with Girdin in breast cancer is unclear. The aim of the present study was to analyze the correlation, expression and nuclear and cytoplasmic localizations of TRAF4 and Girdin in breast cancer tissues. Tissue samples from 38 patients with breast cancer, the MCF‑10A normal mammary epithelial cell line, the MCF‑7 estrogen‑receptor (ER)‑positive and MDA‑MB‑231 ER‑negative breast cancer cell lines were used in the present study. The results demonstrated that cytoplasmic expression of TRAF4 was positively correlated with cytoplasmic expression of Girdin. Furthermore, coexpression of TRAF4 and Girdin was highest in tissue samples from patients with lymph node metastases. Girdin was observed to be predominantly expressed in the cytoplasm of breast cancer cells; however TRAF4 promoted its translocation to the nucleus. These findings suggest that cytoplasmic expression of TRAF4 may be a novel potential marker for cell migration in breast cancer.

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