Astragaloside IV controls collagen reduction in photoaging skin by improving transforming growth factor-β/Smad signaling suppression and inhibiting matrix metalloproteinase-1

Chen,Bin; Li,Ran; Yan,Ning; Chen,Gang; Qian,Wen; Jiang,Hui‑Li; Ji,Chao; Bi,Zhi‑Gang

doi:10.3892/mmr.2015.3212

May-2015 Volume 11 Issue 5

Full Size Image

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

An International Open Access Journal Devoted to General Medicine.

May-2015 Volume 11 Issue 5

Full Size Image

Article Open Access

Astragaloside IV controls collagen reduction in photoaging skin by improving transforming growth factor-β/Smad signaling suppression and inhibiting matrix metalloproteinase-1

Authors:
- Bin Chen
- Ran Li
- Ning Yan
- Gang Chen
- Wen Qian
- Hui‑Li Jiang
- Chao Ji
- Zhi‑Gang Bi
View Affiliations / Copyright

Affiliations: Department of Dermatology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu 210024, P.R. China, Department of Dermatology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350005, P.R. China, Department of Dermatology, BenQ Medical Center, Nanjing Medical University, Nanjing, Jiangsu 210019, P.R. China

Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
Pages: 3344-3348
|
Published online on: January 16, 2015

https://doi.org/10.3892/mmr.2015.3212
Expand metrics +

Abstract

Exposure to ultraviolet (UV) light reduces levels of type I collagen in the dermis and results in human skin damage and premature skin aging (photoaging). This leads to a wrinkled appearance through the inhibition of transforming growth factor‑β (TGF‑β)/Smad signaling. UV irradiation increases type I collagen degradation through upregulating matrix metalloproteinase (MMP) expression. Astragaloside IV (AST) is one of the major active components extracted from Astragalus membranaceus. However, its multiple anti‑photoaging effects remain to be elucidated. In the present study, the effects of AST against collagen reduction in UV‑induced skin aging in human skin fibroblasts were investigated. The expression of type I procollagen (COL1), MMP‑1, TGF‑βRⅡ and Smad7 were determined using reverse transcription‑polymerase chain reaction, western blotting and ELISA, respectively. UV irradiation inhibits type I collagen production by suppressing the TGF‑β/Smad signaling pathway and increasing COL1 degradation by inducing MMP‑1 expression. Transforming growth factor‑β type II protein and COL1 mRNA decreased but MMP‑1 and Smad7 levels increased in the photoaging model group, which was reversed by topical application of AST. AST prevents collagen reduction from UV irradiation in photoaging skin by improving TGF‑β/Smad signaling suppression and inhibiting MMP‑1, thus AST may be a potential agent against skin photoaging.

View Figures

View References

1	Fisher GJ, Wang ZQ, Datta SC, et al: Pathophysiology of premature skin aging induced by ultraviolet light. N Engl J Med. 337:1419–1428. 1997. View Article : Google Scholar : PubMed/NCBI
2	Warren R, Gartstein V, Kligman AM, et al: Age, sunlight, and facial skin: a histologic and quantitative study. J Am Acad Dermatol. 25:751–760. 1991. View Article : Google Scholar : PubMed/NCBI
3	Fisher GJ, Datta SC, Talwar HS, et al: Molecular basis of sun-induced premature skin ageing and retinoid antagonism. Nature. 379:335–339. 1996. View Article : Google Scholar : PubMed/NCBI
4	Talwar HS, Griffiths CE, Fisher GJ, et al: Reduced type I and type III procollagens in photodamaged adult human skin. J Invest Dermatol. 105:285–290. 1995. View Article : Google Scholar : PubMed/NCBI
5	Fisher GJ, Datta S, Wang Z, et al: c-Jun-dependent inhibition of cutaneous procollagen transcription following ultraviolet irradiation is reversed by all-trans retinoic acid. J Clin Invest. 106:663–670. 2000. View Article : Google Scholar : PubMed/NCBI
6	Massagué J: TGF-beta signal transduction. Annu Rev Biochem. 67:753–791. 1998. View Article : Google Scholar : PubMed/NCBI
7	Massagué J and Chen YG: Controlling TGF-beta signaling. Genes Dev. 14:627–644. 2000.PubMed/NCBI
8	Massagué J and Wotton D: Transcriptional control by the TGF-beta/Smad signaling system. EMBO J. 19:1745–1754. 2000. View Article : Google Scholar : PubMed/NCBI
9	Piek E, Heldin CH and Ten Dijke P: Specificity, diversity, and regulation in TGF-beta superfamily signaling. FASEB J. 13:2105–2124. 1999.PubMed/NCBI
10	Quan T, He T, Kang S, Voorhees JJ and Fisher GJ: Solar ultraviolet irradiation reduces collagen in photoaged human skin by blocking transforming growth factor-beta type II receptor/Smad signaling. Am J Pathol. 165:741–751. 2004. View Article : Google Scholar : PubMed/NCBI
11	Chen SJ, Yuan W, Lo S, et al: Interaction of smad3 with a proximal smad-binding element of the human alpha2(I) procollagen gene promoter required for transcriptional activation by TGF-beta. J Cell Physiol. 183:381–392. 2000. View Article : Google Scholar : PubMed/NCBI
12	Ji C, Yang B, Yang YL, et al: Exogenous cell-permeable C6 ceramide sensitizes multiple cancer cell lines to Doxorubicin-induced apoptosis by promoting AMPK activation and mTORC1 inhibition. Oncogene. 29:6557–6568. 2010. View Article : Google Scholar : PubMed/NCBI
13	Inagaki Y, Truter S, Tanaka S, et al: Overlapping pathways mediate the opposing actions of tumor necrosis factor-alpha and transforming growth factor-beta on alpha 2(I) collagen gene transcription. J Biol Chem. 270:3353–3358. 1995. View Article : Google Scholar : PubMed/NCBI
14	Jimenez SA, Varga J, Olsen A, et al: Functional analysis of human alpha 1(I) procollagen gene promoter. Differential activity in collagen-producing and -nonproducing cells and response to transforming growth factor beta 1. J Biol Chem. 269:12684–12691. 1994.PubMed/NCBI
15	Chen SJ, Yuan W, Mori Y, et al: Stimulation of type I collagen transcription in human skin fibroblasts by TGF-beta: involvement of Smad 3. J Invest Dermatol. 112:49–57. 1999. View Article : Google Scholar : PubMed/NCBI
16	Ghosh AK, Yuan W, Mori Y, et al: Smad-dependent stimulation of type I collagen gene expression in human skin fibroblasts by TGF-beta involves functional cooperation with p300/CBP transcriptional coactivators. Oncogene. 19:3546–3555. 2000. View Article : Google Scholar : PubMed/NCBI
17	Ji C, Yang Y, Yang B, et al: Trans-Zeatin attenuates ultraviolet induced down-regulation of aquaporin-3 in cultured human skin keratinocytes. Int J Mol Med. 26:257–263. 2010.PubMed/NCBI
18	Yang B, Ji C, Kang J, et al: Trans-Zeatin inhibits UVB-induced matrix metalloproteinase-1 expression via MAP kinase signaling in human skin fibroblasts. Int J Mol Med. 23:555–560. 2009.PubMed/NCBI

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Astragaloside IV controls collagen reduction in photoaging skin by improving transforming growth factor-β/Smad signaling suppression and inhibiting matrix metalloproteinase-1

This article is mentioned in:

Abstract

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Related Articles