Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
May-2015 Volume 11 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
May-2015 Volume 11 Issue 5

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Inhibition of microRNA-101 attenuates hypoxia/reoxygenation‑induced apoptosis through induction of autophagy in H9c2 cardiomyocytes

  • Authors:
    • Dongkai Wu
    • Haihe Jiang
    • Shengxi Chen
    • Heng Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiothoracic Surgery, Xiangya Hospital of Central South University, Changsha, Hunan 410008, P.R. China
  • Pages: 3988-3994
    |
    Published online on: January 16, 2015
       https://doi.org/10.3892/mmr.2015.3215
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Autophagy is a cellular self‑catabolic process responsible for the degradation of proteins and organelles. Autophagy is able to promote cell survival in response to stress, and increased autophagy amongst cardiomyocytes has been identified in conditions of heart failure, starvation and ischemia/reperfusion. However, the detailed regulatory mechanisms underlying autophagy in heart disease have remained elusive. MicroRNAs (miRNAs) have been implicated in the regulation of autophagy in cells under stress. In the present study, the protective effect of miRNA (miR)‑101 on hypoxia/reoxygenation (H/R)‑induced cardiomyocyte apoptosis was investigated. It was revealed that H/R induced apoptosis in H9c2 cardiomyocytes, accompanied by a downregulation of miR‑101 expression. Further investigation identified Ras‑related protein Rab‑5A (RAB5A) as a direct target of miR‑101. RAB5A was previously reported to be involved in autophagy; therefore, the present study further focused on the role of miR‑101 in the regulation of autophagy under H/R and found that the inhibition of miR‑101 attenuated H/R‑induced apoptosis, at least partially, via the induction of autophagy. In conclusion, the results of the present study revealed a beneficial effect of miR‑101 inhibition on H/R‑induced apoptosis in cardiomyocytes, indicating that miR‑101 inhibition may present a potential therapeutic agent in the treatment or prevention of heart diseases.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Oku M, Takano Y and Sakai Y: The emerging role of autophagy in peroxisome dynamics and lipid metabolism of phyllosphere microorganisms. Front Plant Sci. 5:812014. View Article : Google Scholar : PubMed/NCBI

2 

Petersen M, Hofius D and Andersen SU: Signaling unmasked: Autophagy and catalase promote programmed cell death. Autophagy. 10:2004.

3 

Tessitore A, Cicciarelli G, Del Vecchio F, et al: MicroRNAs in the DNA Damage/Repair Network and Cancer. Int J Genomics. 2014:8202482014. View Article : Google Scholar : PubMed/NCBI

4 

Chen LJ, Lim SH, Yeh YT, Lien SC and Chiu JJ: Roles of microRNAs in atherosclerosis and restenosis. J Biomed Sci. 19:792012. View Article : Google Scholar : PubMed/NCBI

5 

Xu T, Li D and Jiang D: Targeting cell signaling and apoptotic pathways by luteolin: cardioprotective role in rat cardiomyocytes following ischemia/reperfusion. Nutrients. 4:2008–2019. 2012. View Article : Google Scholar : PubMed/NCBI

6 

Morita K: Surgical reoxygenation injury of the myocardium in cyanotic patients: clinical relevance and therapeutic strategies by normoxic management during cardiopulmonary bypass. Gen Thorac Cardiovasc Surg. 60:549–556. 2012. View Article : Google Scholar : PubMed/NCBI

7 

Liu LF, Liang Z, Lv ZR, et al: MicroRNA-15a/b are up-regulated in response to myocardial ischemia/reperfusion injury. J Geriatr Cardiol. 9:28–32. 2012. View Article : Google Scholar : PubMed/NCBI

8 

Qin Y, Yu Y, Dong H, Bian X, Guo X and Dong S: MicroRNA 21 inhibits left ventricular remodeling in the early phase of rat model with ischemia-reperfusion injury by suppressing cell apoptosis. Int J Med Sci. 9:413–423. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Liu L, Zhang G, Liang Z, et al: MicroRNA-15b enhances hypoxia/reoxygenation-induced apoptosis of cardiomyocytes via a mitochondrial apoptotic pathway. Apoptosis. 19:19–29. 2014. View Article : Google Scholar

10 

Frankel LB, Wen J, Lees M, et al: microRNA-101 is a potent inhibitor of autophagy. EMBO J. 30:4628–4641. 2011. View Article : Google Scholar : PubMed/NCBI

11 

Lin X, Guan H, Li H, et al: miR-101 inhibits cell proliferation by targeting Rac1 in papillary thyroid carcinoma. Biomed Rep. 2:122–126. 2014.PubMed/NCBI

12 

Yin J, Wang M, Jin C and Qi Q: miR-101 sensitizes A549 NSCLC cell line to CDDP by activating caspase 3-dependent apoptosis. Oncol Lett. 7:461–465. 2014.PubMed/NCBI

13 

Gong JS and Kim GJ: he role of autophagy in the placenta as a regulator of cell death. Clin Exp Reprod Med. 41:97–107. 2014. View Article : Google Scholar : PubMed/NCBI

14 

Hou C, Zhu M, Sun M and Lin Y: MicroRNA let-7i induced autophagy to protect T cell from apoptosis by targeting IGF1R. Biochem Biophys Res Commun. 453:728–734. 2014. View Article : Google Scholar : PubMed/NCBI

15 

Gustafsson AB and Gottlieb RA: Recycle or die: the role of autophagy in cardioprotection. J Mol Cell Cardiol. 44:654–661. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Xu Y, An Y, Wang Y, et al: miR-101 inhibits autophagy and enhances cisplatin-induced apoptosis in hepatocellular carcinoma cells. Oncol Rep. 29:2019–2024. 2013.PubMed/NCBI

17 

Li Y, Zhao Y, Hu J, et al: A novel ER-localized transmembrane protein, EMC6, interacts with RAB5A and regulates cell autophagy. Autophagy. 9:150–163. 2013. View Article : Google Scholar :

18 

Sybers HD, Ingwall J and DeLuca M: Autophagy in cardiac myocytes. Recent Adv Stud Cardiac Struct Metab. 12:453–463. 1976.PubMed/NCBI

19 

Zhang ZL, Fan Y and Liu ML: Ginsenoside Rg1 inhibits autophagy in H9c2 cardiomyocytes exposed to hypoxia/reoxygenation. Mol Cell Biochem. 365:243–250. 2012. View Article : Google Scholar : PubMed/NCBI

20 

Cao X, Chen A, Yang P, et al: Alpha-lipoic acid protects cardiomyocytes against hypoxia/reoxygenation injury by inhibiting autophagy. Biochem Biophys Res Commun. 441:935–940. 2013. View Article : Google Scholar : PubMed/NCBI

21 

Wang JH, Behrns KE, Leeuwenburgh C and Kim JS: Critical role of autophage in ischemia/reperfusion injury to aged livers. Autophagy. 8:140–141. 2012. View Article : Google Scholar :

22 

Han Z, Cao J, Song D, et al: Autophagy is involved in the cardioprotection effect of remote limb ischemic postconditioning on myocardial ischemia/reperfusion injury in normal mice, but not diabetic mice. PLoS One. 9:e868382014. View Article : Google Scholar : PubMed/NCBI

23 

Kang JW, Cho H and Lee SM: Melatonin inhibits mTOR-dependent autophagy during liver ischemia/reperfusion. Cell Physiol Biochem. 33:23–36. 2014. View Article : Google Scholar : PubMed/NCBI

24 

Zhang Y and Ren J: Targeting autophagy for the therapeutic application of histone deacetylase inhibitors in ischemia/reperfusion heart injury. Circulation. 129:1088–1091. 2014. View Article : Google Scholar : PubMed/NCBI

25 

Zeng M, Wei X, Wu Z, et al: NF-κB-mediated induction of autophagy in cardiac ischemia/reperfusion injury. Biochem Biophys Res Commun. 436:180–185. 2013. View Article : Google Scholar : PubMed/NCBI

26 

Hamacher-Brady A, Brady NR and Gottlieb RA: The interplay between pro-death and pro-survival signaling pathways in myocardial ischemia/reperfusion injury: apoptosis meets autophagy. Cardiovasc Drugs Ther. 20:445–462. 2006. View Article : Google Scholar : PubMed/NCBI

27 

Liu G, Wang T, Song J and Zhou Z: Effects of apoptosis-related proteins caspase-3, Bax and Bcl-2 on cerebral ischemia rats. Biomed Rep. 1:861–867. 2013.

28 

Ni Z, Wang B, Dai X, et al: HCC cells with high levels of Bcl-2 are resistant to ABT-737 via activation of the ROS-JNK-autophagy pathway. Free Radic Biol Med. 70:194–203. 2014. View Article : Google Scholar : PubMed/NCBI

29 

Heath-Engel HM, Chang NC and Shore GC: The endoplasmic reticulum in apoptosis and autophagy: role of the BCL-2 protein family. Oncogene. 27:6419–6433. 2008. View Article : Google Scholar : PubMed/NCBI

30 

Liang XH, Kleeman LK, Jiang HH, et al: Protection against fatal Sindbis virus encephalitis by beclin, a novel Bcl-2-interacting protein. J Virol. 72:8586–8596. 1998.PubMed/NCBI

31 

Pattingre S, Tassa A, Qu X, et al: Bcl-2 antiapoptotic proteins inhibit Beclin 1-dependent autophagy. Cell. 122:927–939. 2005. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Wu D, Jiang H, Chen S and Zhang H: Inhibition of microRNA-101 attenuates hypoxia/reoxygenation‑induced apoptosis through induction of autophagy in H9c2 cardiomyocytes. Mol Med Rep 11: 3988-3994, 2015.
APA
Wu, D., Jiang, H., Chen, S., & Zhang, H. (2015). Inhibition of microRNA-101 attenuates hypoxia/reoxygenation‑induced apoptosis through induction of autophagy in H9c2 cardiomyocytes. Molecular Medicine Reports, 11, 3988-3994. https://doi.org/10.3892/mmr.2015.3215
MLA
Wu, D., Jiang, H., Chen, S., Zhang, H."Inhibition of microRNA-101 attenuates hypoxia/reoxygenation‑induced apoptosis through induction of autophagy in H9c2 cardiomyocytes". Molecular Medicine Reports 11.5 (2015): 3988-3994.
Chicago
Wu, D., Jiang, H., Chen, S., Zhang, H."Inhibition of microRNA-101 attenuates hypoxia/reoxygenation‑induced apoptosis through induction of autophagy in H9c2 cardiomyocytes". Molecular Medicine Reports 11, no. 5 (2015): 3988-3994. https://doi.org/10.3892/mmr.2015.3215
Copy and paste a formatted citation
x
Spandidos Publications style
Wu D, Jiang H, Chen S and Zhang H: Inhibition of microRNA-101 attenuates hypoxia/reoxygenation‑induced apoptosis through induction of autophagy in H9c2 cardiomyocytes. Mol Med Rep 11: 3988-3994, 2015.
APA
Wu, D., Jiang, H., Chen, S., & Zhang, H. (2015). Inhibition of microRNA-101 attenuates hypoxia/reoxygenation‑induced apoptosis through induction of autophagy in H9c2 cardiomyocytes. Molecular Medicine Reports, 11, 3988-3994. https://doi.org/10.3892/mmr.2015.3215
MLA
Wu, D., Jiang, H., Chen, S., Zhang, H."Inhibition of microRNA-101 attenuates hypoxia/reoxygenation‑induced apoptosis through induction of autophagy in H9c2 cardiomyocytes". Molecular Medicine Reports 11.5 (2015): 3988-3994.
Chicago
Wu, D., Jiang, H., Chen, S., Zhang, H."Inhibition of microRNA-101 attenuates hypoxia/reoxygenation‑induced apoptosis through induction of autophagy in H9c2 cardiomyocytes". Molecular Medicine Reports 11, no. 5 (2015): 3988-3994. https://doi.org/10.3892/mmr.2015.3215
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team