miR-101 sensitizes human nasopharyngeal carcinoma cells to radiation by targeting stathmin 1

Sun,Quanquan; Liu,Tongxin; Zhang,Tian; Du,Shasha; Xie,Guozhu Xie; Lin,Xiaoshan; Chen,Longhua; Yuan,Yawei

doi:10.3892/mmr.2015.3221

miR-101 sensitizes human nasopharyngeal carcinoma cells to radiation by targeting stathmin 1

Authors:
- Quanquan Sun
- Tongxin Liu
- Tian Zhang
- Shasha Du
- Guozhu Xie Xie
- Xiaoshan Lin
- Longhua Chen
- Yawei Yuan
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Affiliations: Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong 510515, P.R. China
Published online on: January 19, 2015 https://doi.org/10.3892/mmr.2015.3221
Pages: 3330-3336
Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].

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Abstract

Radioresistance remains a major problem in the treatment of patients suffering from nasopharyngeal carcinoma (NPC). A better understanding of the mechanisms involved in the induction of radioresistance may provide strategies to improve NPC patients' response to therapy. The present study aimed to investigate the effect of microRNA (miR)-101 on the radioresistance of NPC cells. Analysis of miR-101 expression levels indicated that miR-101 was downregulated in NPC cell lines. Furthermore, ectopic expression of miR-101 suppressed cell proliferation and enhanced radiosensitivity of NPC cells. Stathmin 1 (STMN1) was additionally verified as a direct functional target of miR-101, which was found to be involved in cell viability, radioresistance and radiation-induced autophagy of NPC cells. In conclusions, the results of the present study suggested that the identified miR-101/STMN1 pathway contributed to the elucidation of the mechanisms of radioresistance in human NPC and that it may represent a potential therapeutic target.

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