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Article

Fucoidan protects ARPE-19 cells from oxidative stress via normalization of reactive oxygen species generation through the Ca2+-dependent ERK signaling pathway

  • Authors:
    • Xiaoxia Li
    • Haiyan Zhao
    • Qingfa Wang
    • Hongyan Liang
    • Xiaofeng Jiang
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Biochemistry Laboratory, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China, Department of Ophthalmology, Central Hospital of Liaoyang City, Liaoyang, Liaoning 111000, P.R. China, Department of Emergency, The First Affiliated Hospital of Jiamusi University, Jiamusi, Heilongjiang 154002, P.R. China
  • Pages: 3746-3752
    |
    Published online on: January 19, 2015
       https://doi.org/10.3892/mmr.2015.3224
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Abstract

Diabetic retinopathy (DR) is a common complication of diabetes mellitus (DM) and it is the main cause of loss of vision. In previous years, interest in the biological activities of marine organisms has intensified. The effect of fucoidan from the seaweed Fucus vesiculosus on the molecular mechanisms of numerous diseases has been studied, while to date, its effect on DR was yet to be investigated. Therefore, the aim of the present study was to evaluate the role of fucoidan in DR. The human retinal pigment epithelial cell line ARPE‑19 was exposed to high D‑glucose in the presence or absence of fucoidan. Cell viability was monitored using MTT and lactate dehydrogenase assays. The intracellular reactive oxygen species (ROS) generation was measured using fluorescence spectrophotometry. Cell apoptosis was measured by flow cytometry using Annexin V‑fluorescein isothiocyanate staining. Ca2+ influx was measured with a calcium imaging system and the activation of the extracellular signal‑regulated kinase (ERK) protein was evaluated using western blot analysis. The non‑toxic fucoidan protected ARPE‑19 cells from high glucose‑induced cell death and normalized high glucose‑induced generation of ROS. Fucoidan also inhibited high glucose‑induced cell apoptosis, as well as the Ca2+ influx and ERK1/2 phosphorylation in ARPE‑19 cells. Taken together, these findings indicated that fucoidan protects ARPE‑19 cells against high glucose‑induced oxidative damage via normalization of ROS generation through the Ca2+‑dependent ERK signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Li X, Zhao H, Wang Q, Liang H and Jiang X: Fucoidan protects ARPE-19 cells from oxidative stress via normalization of reactive oxygen species generation through the Ca2+-dependent ERK signaling pathway. Mol Med Rep 11: 3746-3752, 2015.
APA
Li, X., Zhao, H., Wang, Q., Liang, H., & Jiang, X. (2015). Fucoidan protects ARPE-19 cells from oxidative stress via normalization of reactive oxygen species generation through the Ca2+-dependent ERK signaling pathway. Molecular Medicine Reports, 11, 3746-3752. https://doi.org/10.3892/mmr.2015.3224
MLA
Li, X., Zhao, H., Wang, Q., Liang, H., Jiang, X."Fucoidan protects ARPE-19 cells from oxidative stress via normalization of reactive oxygen species generation through the Ca2+-dependent ERK signaling pathway". Molecular Medicine Reports 11.5 (2015): 3746-3752.
Chicago
Li, X., Zhao, H., Wang, Q., Liang, H., Jiang, X."Fucoidan protects ARPE-19 cells from oxidative stress via normalization of reactive oxygen species generation through the Ca2+-dependent ERK signaling pathway". Molecular Medicine Reports 11, no. 5 (2015): 3746-3752. https://doi.org/10.3892/mmr.2015.3224
Copy and paste a formatted citation
x
Spandidos Publications style
Li X, Zhao H, Wang Q, Liang H and Jiang X: Fucoidan protects ARPE-19 cells from oxidative stress via normalization of reactive oxygen species generation through the Ca2+-dependent ERK signaling pathway. Mol Med Rep 11: 3746-3752, 2015.
APA
Li, X., Zhao, H., Wang, Q., Liang, H., & Jiang, X. (2015). Fucoidan protects ARPE-19 cells from oxidative stress via normalization of reactive oxygen species generation through the Ca2+-dependent ERK signaling pathway. Molecular Medicine Reports, 11, 3746-3752. https://doi.org/10.3892/mmr.2015.3224
MLA
Li, X., Zhao, H., Wang, Q., Liang, H., Jiang, X."Fucoidan protects ARPE-19 cells from oxidative stress via normalization of reactive oxygen species generation through the Ca2+-dependent ERK signaling pathway". Molecular Medicine Reports 11.5 (2015): 3746-3752.
Chicago
Li, X., Zhao, H., Wang, Q., Liang, H., Jiang, X."Fucoidan protects ARPE-19 cells from oxidative stress via normalization of reactive oxygen species generation through the Ca2+-dependent ERK signaling pathway". Molecular Medicine Reports 11, no. 5 (2015): 3746-3752. https://doi.org/10.3892/mmr.2015.3224
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