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Article Open Access

Enhanced specific antitumor immunity of dendritic cells transduced with the glypican 3 gene and co-cultured with cytokine-induced killer cells against hepatocellular carcinoma cells

  • Authors:
    • Yuliang Wang
    • Yinlong Wang
    • Hong Mu
    • Tao Liu
    • Xiaobo Chen
    • Zhongyang Shen
  • View Affiliations / Copyright

    Affiliations: Department of Clinical Laboratory Medicine, Tianjin First Central Hospital, Key Laboratory for Critical Care Medicine of the Ministry of Health, Tianjin 300192, P.R. China, Department of Hernia and Abdominal Wall Surgery, Union Medicine Center, Tianjin 300121, P.R. China, Union Stem and Gene Engineering Co., Tianjin 300384, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License [CC BY_NC 3.0].
  • Pages: 3361-3367
    |
    Published online on: January 22, 2015
       https://doi.org/10.3892/mmr.2015.3239
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Abstract

Dendritic cell (DC)‑based cancer immunotherapy requires an immunogenic tumor‑associated antigen and an effective therapeutic strategy. Glypican 3 (GPC3) is a valuable diagnostic marker and a potential therapeutic target in hepatocellular carcinoma (HCC). The present study investigated whether DCs transduced with the GPC3 gene (DCs‑GPC3) and co‑cultured with autologous cytokine‑induced killer cells (CIKs) may induce a marked specific immune response against GPC3‑expressing HCC cells in vitro and in vivo. Human DCs were transfected with a green fluorescent protein plasmid with GPC3 by nucleofection and then co‑cultured with autologous CIKs. Flow cytometry was used to measure the phenotypes of DCs and CIKs. The co‑cultured cells were harvested and incubated with HCC cells and the cytotoxicity of the CIKs was assessed by nonradioactive cytotoxicity assay. The anti-tumor activity of these effector cells was further evaluated using a nude mouse tumor model. The results demonstrated that DCs‑GPC3 significantly promoted the autologous CIKs differentiation, as well as anti‑tumor cytokine interferon‑γ secretion. In addition, DCs‑GPC3‑CIKs significantly enhanced the cytotoxic activity against GPC3‑expressing HepG2 cells, indicating a GPC3‑specific marked immune response against HCC cells. The in vivo data indicated that DCs‑GPC3‑CIKs exhibited significant HepG2 cell‑induced tumor growth inhibition in nude mice. The results of the present study provided a new insight into the design of personalizing adoptive immunotherapy for GPC3‑expressing HCC cells.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Wang Y, Mu H, Liu T, Chen X and Shen Z: Enhanced specific antitumor immunity of dendritic cells transduced with the glypican 3 gene and co-cultured with cytokine-induced killer cells against hepatocellular carcinoma cells. Mol Med Rep 11: 3361-3367, 2015.
APA
Wang, Y., Wang, Y., Mu, H., Liu, T., Chen, X., & Shen, Z. (2015). Enhanced specific antitumor immunity of dendritic cells transduced with the glypican 3 gene and co-cultured with cytokine-induced killer cells against hepatocellular carcinoma cells. Molecular Medicine Reports, 11, 3361-3367. https://doi.org/10.3892/mmr.2015.3239
MLA
Wang, Y., Wang, Y., Mu, H., Liu, T., Chen, X., Shen, Z."Enhanced specific antitumor immunity of dendritic cells transduced with the glypican 3 gene and co-cultured with cytokine-induced killer cells against hepatocellular carcinoma cells". Molecular Medicine Reports 11.5 (2015): 3361-3367.
Chicago
Wang, Y., Wang, Y., Mu, H., Liu, T., Chen, X., Shen, Z."Enhanced specific antitumor immunity of dendritic cells transduced with the glypican 3 gene and co-cultured with cytokine-induced killer cells against hepatocellular carcinoma cells". Molecular Medicine Reports 11, no. 5 (2015): 3361-3367. https://doi.org/10.3892/mmr.2015.3239
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Wang Y, Mu H, Liu T, Chen X and Shen Z: Enhanced specific antitumor immunity of dendritic cells transduced with the glypican 3 gene and co-cultured with cytokine-induced killer cells against hepatocellular carcinoma cells. Mol Med Rep 11: 3361-3367, 2015.
APA
Wang, Y., Wang, Y., Mu, H., Liu, T., Chen, X., & Shen, Z. (2015). Enhanced specific antitumor immunity of dendritic cells transduced with the glypican 3 gene and co-cultured with cytokine-induced killer cells against hepatocellular carcinoma cells. Molecular Medicine Reports, 11, 3361-3367. https://doi.org/10.3892/mmr.2015.3239
MLA
Wang, Y., Wang, Y., Mu, H., Liu, T., Chen, X., Shen, Z."Enhanced specific antitumor immunity of dendritic cells transduced with the glypican 3 gene and co-cultured with cytokine-induced killer cells against hepatocellular carcinoma cells". Molecular Medicine Reports 11.5 (2015): 3361-3367.
Chicago
Wang, Y., Wang, Y., Mu, H., Liu, T., Chen, X., Shen, Z."Enhanced specific antitumor immunity of dendritic cells transduced with the glypican 3 gene and co-cultured with cytokine-induced killer cells against hepatocellular carcinoma cells". Molecular Medicine Reports 11, no. 5 (2015): 3361-3367. https://doi.org/10.3892/mmr.2015.3239
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