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Article

Protective effect of Porphyra yezoensis glycoprotein on D-galactosamine‑induced cytotoxicity in Hepa 1c1c7 cells

  • Authors:
    • Jeong‑Wook Choi
    • Young‑Min Kim
    • Su‑Jin Park
    • In‑Hye Kim
    • Taek‑Jeong Nam
  • View Affiliations / Copyright

    Affiliations: Department of Food and Life Science, Pukyong National University, Nam‑gu, Busan 608‑737, Republic of Korea
  • Pages: 3914-3919
    |
    Published online on: January 23, 2015
       https://doi.org/10.3892/mmr.2015.3244
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Abstract

The present study aimed to examine the signaling pathways and enzyme activity associated with the protective effect of Porphyra yezoensis glycoprotein (PYGP) on D‑galactosamine (D‑GaIN)‑induced cytotoxicity in Hepa 1c1c7 cells. D‑GaIN is commonly used to induce hepatic injury models in vivo as well as in vitro. PYGP was extracted from Porphyra yezoensis, a red algae distributed along the coasts of Republic of Korea, China and Japan. In the present study, Hepa 1c1c7 cells were pre‑treated with PYGP (20 and 40 µg/ml) for 24 h and then the media was replaced with D‑GaIN (20 mM) and PYGP (20 and 40 µg/ml). The results demonstrated that D‑GaIN induced Hepa 1c1c7 cell death and pretreatment with PYGP was found to attenuate D‑GaIN toxicity. In addition, D‑GaIN decreased the antioxidant activity and increased lipid peroxidation processes; however, pre‑treatment with PYGP reduced the generation of lipid peroxidation products, such as thiobarbituric acid reactive substances, as well as increased the activity of antioxidant enzymes, including superoxide dismutase, catalase and glutathione‑s‑transferase (GST). PYGP was shown to suppress the overexpression of extracellular signal‑regulated kinase, c‑jun N‑terminal kinase and p38 mitogen‑activated protein kinase (MAPK) phosphorylation induced by D‑GaIN. Furthermore, PYGP increased the protein expression of nuclear factor erythroid 2‑related factor 2 (Nrf2), quinine oxidoreductase 1, GST and heme oxygenase 1 protein expression. These results suggested that PYGP had cytoprotective effects against D‑GaIN‑induced cell damage, which may be associated with MAPKs and the Nrf2 signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Choi JW, Kim YM, Park SJ, Kim IH and Nam TJ: Protective effect of Porphyra yezoensis glycoprotein on D-galactosamine‑induced cytotoxicity in Hepa 1c1c7 cells. Mol Med Rep 11: 3914-3919, 2015.
APA
Choi, J., Kim, Y., Park, S., Kim, I., & Nam, T. (2015). Protective effect of Porphyra yezoensis glycoprotein on D-galactosamine‑induced cytotoxicity in Hepa 1c1c7 cells. Molecular Medicine Reports, 11, 3914-3919. https://doi.org/10.3892/mmr.2015.3244
MLA
Choi, J., Kim, Y., Park, S., Kim, I., Nam, T."Protective effect of Porphyra yezoensis glycoprotein on D-galactosamine‑induced cytotoxicity in Hepa 1c1c7 cells". Molecular Medicine Reports 11.5 (2015): 3914-3919.
Chicago
Choi, J., Kim, Y., Park, S., Kim, I., Nam, T."Protective effect of Porphyra yezoensis glycoprotein on D-galactosamine‑induced cytotoxicity in Hepa 1c1c7 cells". Molecular Medicine Reports 11, no. 5 (2015): 3914-3919. https://doi.org/10.3892/mmr.2015.3244
Copy and paste a formatted citation
x
Spandidos Publications style
Choi JW, Kim YM, Park SJ, Kim IH and Nam TJ: Protective effect of Porphyra yezoensis glycoprotein on D-galactosamine‑induced cytotoxicity in Hepa 1c1c7 cells. Mol Med Rep 11: 3914-3919, 2015.
APA
Choi, J., Kim, Y., Park, S., Kim, I., & Nam, T. (2015). Protective effect of Porphyra yezoensis glycoprotein on D-galactosamine‑induced cytotoxicity in Hepa 1c1c7 cells. Molecular Medicine Reports, 11, 3914-3919. https://doi.org/10.3892/mmr.2015.3244
MLA
Choi, J., Kim, Y., Park, S., Kim, I., Nam, T."Protective effect of Porphyra yezoensis glycoprotein on D-galactosamine‑induced cytotoxicity in Hepa 1c1c7 cells". Molecular Medicine Reports 11.5 (2015): 3914-3919.
Chicago
Choi, J., Kim, Y., Park, S., Kim, I., Nam, T."Protective effect of Porphyra yezoensis glycoprotein on D-galactosamine‑induced cytotoxicity in Hepa 1c1c7 cells". Molecular Medicine Reports 11, no. 5 (2015): 3914-3919. https://doi.org/10.3892/mmr.2015.3244
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