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Article

Puerarin attenuates myocardial hypoxia/reoxygenation injury by inhibiting autophagy via the Akt signaling pathway

  • Authors:
    • Huixiong Tang
    • Xudong Song
    • Yuanna Ling
    • Xianbao Wang
    • Pingzhen Yang
    • Tao Luo
    • Aihua Chen
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Zhujiang Hospital, Southern Medical University, Guangzhou, Guangdong 510282, P.R. China, Division of Cardiology, Department of Medicine, University of California Irvine Medical Center, Orange, CA 92868, USA
  • Pages: 3747-3754
    |
    Published online on: April 3, 2017
       https://doi.org/10.3892/mmr.2017.6424
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Abstract

Puerarin (Pur), which is the major bioactive ingredient extracted from the root of Pueraria lobata (Willd.) Ohwi, has been demonstrated to relieve myocardial ischemia/reperfusion (I/R) injury. Macroautophagy, or autophagy, is an evolutionarily conserved cellular catabolic mechanism that is involved in myocardial I/R injury. The present study evaluated the involvement of autophagy in the protective mechanisms of Pur during myocardial hypoxia/reoxygenation (H/R). The results revealed that Pur and 3‑methyladenine pretreatment exerted a cardioprotective effect against H/R‑induced cell viability loss. Pur also decreased the ratio of light chain 3 (LC3) ‑II/LC3‑I and the degradation of p62 during H/R, which was accompanied by an increased level of phosphorylated‑protein kinase B (Akt). These findings suggested that autophagy during myocardial H/R was inhibited by Pur, and this was further confirmed by the results of transmission electron microscopy and adenovirus‑monomeric red fluorescent protein‑green fluorescent protein‑light chain 3 transfection. Furthermore, Pur inhibited the increased levels of autophagy induced by rapamycin, and the autophagy‑inhibiting effects of Pur during myocardial H/R were abolished by the Akt signaling inhibitor API‑2. Collectively, these data indicate that Pur pretreatment may attenuate myocardial H/R injury by inhibiting autophagy via the Akt signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Tang H, Song X, Ling Y, Wang X, Yang P, Luo T and Chen A: Puerarin attenuates myocardial hypoxia/reoxygenation injury by inhibiting autophagy via the Akt signaling pathway. Mol Med Rep 15: 3747-3754, 2017.
APA
Tang, H., Song, X., Ling, Y., Wang, X., Yang, P., Luo, T., & Chen, A. (2017). Puerarin attenuates myocardial hypoxia/reoxygenation injury by inhibiting autophagy via the Akt signaling pathway. Molecular Medicine Reports, 15, 3747-3754. https://doi.org/10.3892/mmr.2017.6424
MLA
Tang, H., Song, X., Ling, Y., Wang, X., Yang, P., Luo, T., Chen, A."Puerarin attenuates myocardial hypoxia/reoxygenation injury by inhibiting autophagy via the Akt signaling pathway". Molecular Medicine Reports 15.6 (2017): 3747-3754.
Chicago
Tang, H., Song, X., Ling, Y., Wang, X., Yang, P., Luo, T., Chen, A."Puerarin attenuates myocardial hypoxia/reoxygenation injury by inhibiting autophagy via the Akt signaling pathway". Molecular Medicine Reports 15, no. 6 (2017): 3747-3754. https://doi.org/10.3892/mmr.2017.6424
Copy and paste a formatted citation
x
Spandidos Publications style
Tang H, Song X, Ling Y, Wang X, Yang P, Luo T and Chen A: Puerarin attenuates myocardial hypoxia/reoxygenation injury by inhibiting autophagy via the Akt signaling pathway. Mol Med Rep 15: 3747-3754, 2017.
APA
Tang, H., Song, X., Ling, Y., Wang, X., Yang, P., Luo, T., & Chen, A. (2017). Puerarin attenuates myocardial hypoxia/reoxygenation injury by inhibiting autophagy via the Akt signaling pathway. Molecular Medicine Reports, 15, 3747-3754. https://doi.org/10.3892/mmr.2017.6424
MLA
Tang, H., Song, X., Ling, Y., Wang, X., Yang, P., Luo, T., Chen, A."Puerarin attenuates myocardial hypoxia/reoxygenation injury by inhibiting autophagy via the Akt signaling pathway". Molecular Medicine Reports 15.6 (2017): 3747-3754.
Chicago
Tang, H., Song, X., Ling, Y., Wang, X., Yang, P., Luo, T., Chen, A."Puerarin attenuates myocardial hypoxia/reoxygenation injury by inhibiting autophagy via the Akt signaling pathway". Molecular Medicine Reports 15, no. 6 (2017): 3747-3754. https://doi.org/10.3892/mmr.2017.6424
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