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Article Open Access

Prognostic role of TPL2 in early‑stage non‑small cell lung cancer

  • Authors:
    • Laura Boldrini
    • Mirella Giordano
    • Adele Servadio
    • Cristina Niccoli
    • Pietro Bertoglio
    • Marco Lucchi
    • Franca Melfi
    • Alfredo Mussi
    • Gabriella Fontanini
  • View Affiliations / Copyright

    Affiliations: Department of Surgical, Medical, Molecular Pathology and Critical Care, University of Pisa, I‑56126 Pisa, Italy, Unit of Thoracic Surgery, Azienda Ospedaliero‑Universitaria Pisana, I‑56126 Pisa, Italy
    Copyright: © Boldrini et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3451-3458
    |
    Published online on: April 3, 2017
       https://doi.org/10.3892/mmr.2017.6430
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Abstract

Non‑small cell lung cancer (NSCLC) accounts for ~70% of all lung cancer‑associated mortalities worldwide. The serine/threonine protein kinase tumor progression locus 2 [TPL2/MAP3 kinase 8 (MAP3K8)] may impact oncogenic events; however the role of TPL2 in lung carcinogenesis remains unclear. The present study was focused on the potential prognostic role of TPL2 in 101 patients with early‑stage NSCLC. Since TPL2 is a potential target of miR‑21, the association between TPL2 and miR‑21 expression was also examined. TPL2 and miR‑21 mRNA expression was quantified using reverse transcription quantitative polymerase chain reaction (RT‑qPCR). TPL2 protein levels were evaluated by immunohistochemistry (IHC). The present study identified that the mRNA expression of TPL2 was low in 52/101 (51%) cases and high in 49/101 (49%) cases. IHC analysis of TPL2 protein expression often demonstrated identical mRNA results. No statistically significant associations were observed between the mRNA expression of TPL2 and the predominant clinicopathological characteristics of the patients with NSCLC, as well as identifying no association between TPL2 and miR‑21. TPL2 mRNA expression was significantly higher in patients with NSCLC with good prognosis (disease‑free interval P=0.009; overall survival P=0.024), when compared with those of poor prognosis. Focusing on the difference in mRNA expression of TPL2 among the adenocarcinomas in affected patients, TPL2 was more highly expressed in lepidic adenocarcinomas compared with in the other subtypes (P=0.012). The present study is the first examination, to the best of our knowledge, of TPL2 in early‑stage NSCLC in relation to miR‑21, and in different adenocarcinoma subtypes. Future studies must clarify the mechanism by which TPL2 is involved in lung carcinogenesis due to its important translational implications.
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Copy and paste a formatted citation
Spandidos Publications style
Boldrini L, Giordano M, Servadio A, Niccoli C, Bertoglio P, Lucchi M, Melfi F, Mussi A and Fontanini G: Prognostic role of TPL2 in early‑stage non‑small cell lung cancer. Mol Med Rep 15: 3451-3458, 2017.
APA
Boldrini, L., Giordano, M., Servadio, A., Niccoli, C., Bertoglio, P., Lucchi, M. ... Fontanini, G. (2017). Prognostic role of TPL2 in early‑stage non‑small cell lung cancer. Molecular Medicine Reports, 15, 3451-3458. https://doi.org/10.3892/mmr.2017.6430
MLA
Boldrini, L., Giordano, M., Servadio, A., Niccoli, C., Bertoglio, P., Lucchi, M., Melfi, F., Mussi, A., Fontanini, G."Prognostic role of TPL2 in early‑stage non‑small cell lung cancer". Molecular Medicine Reports 15.6 (2017): 3451-3458.
Chicago
Boldrini, L., Giordano, M., Servadio, A., Niccoli, C., Bertoglio, P., Lucchi, M., Melfi, F., Mussi, A., Fontanini, G."Prognostic role of TPL2 in early‑stage non‑small cell lung cancer". Molecular Medicine Reports 15, no. 6 (2017): 3451-3458. https://doi.org/10.3892/mmr.2017.6430
Copy and paste a formatted citation
x
Spandidos Publications style
Boldrini L, Giordano M, Servadio A, Niccoli C, Bertoglio P, Lucchi M, Melfi F, Mussi A and Fontanini G: Prognostic role of TPL2 in early‑stage non‑small cell lung cancer. Mol Med Rep 15: 3451-3458, 2017.
APA
Boldrini, L., Giordano, M., Servadio, A., Niccoli, C., Bertoglio, P., Lucchi, M. ... Fontanini, G. (2017). Prognostic role of TPL2 in early‑stage non‑small cell lung cancer. Molecular Medicine Reports, 15, 3451-3458. https://doi.org/10.3892/mmr.2017.6430
MLA
Boldrini, L., Giordano, M., Servadio, A., Niccoli, C., Bertoglio, P., Lucchi, M., Melfi, F., Mussi, A., Fontanini, G."Prognostic role of TPL2 in early‑stage non‑small cell lung cancer". Molecular Medicine Reports 15.6 (2017): 3451-3458.
Chicago
Boldrini, L., Giordano, M., Servadio, A., Niccoli, C., Bertoglio, P., Lucchi, M., Melfi, F., Mussi, A., Fontanini, G."Prognostic role of TPL2 in early‑stage non‑small cell lung cancer". Molecular Medicine Reports 15, no. 6 (2017): 3451-3458. https://doi.org/10.3892/mmr.2017.6430
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