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Removal from adherent culture contributes to apoptosis in human bone marrow mesenchymal stem cells

  • Authors:
    • Baoping Deng
    • Haiming Jiang
    • Kuan Zeng
    • Yi Liang
    • Yinmeng Wu
    • Yanqi Yang
  • View Affiliations / Copyright

    Affiliations: Department of Cardiovascular Surgery, Sun Yat‑sen Memorial Hospital, Sun Yat‑sen University, Guangzhou, Guangdong 510120, P.R. China, Department of Cardiothoracic Surgery, Zhongshan People's Hospital, Zhongshan, Guangdong 528403, P.R. China
    Copyright: © Deng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3499-3506
    |
    Published online on: April 7, 2017
       https://doi.org/10.3892/mmr.2017.6440
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Abstract

Mesenchymal stem cells (MSCs) are routinely isolated due to their adherence to tissue culture plates and their in vitro growth characteristics. Expansion of MSCs in adherent cultures is the only way to obtain sufficient cells for use in either clinical or research settings. MSCs have tremendous potential in myocardial repair treatment by cell therapy techniques, however, a large number of MSCs die from apoptosis following transplantation. Previous studies have examined the factors contributing to the survival of transplanted cells, but little is known about the effect of removal from adherent culture conditions on apoptosis of the MSCs. In the present study, human bone marrow MSCs were expanded in adherent cultures. Then apoptosis rates were examined at different time points in MSCs cultured in nonadherent conditions (ultra‑low‑adherence plates) compared with MSCs cultured in adherent conditions (standard tissue culture plates). Flow cytometry analysis suggested that cell apoptosis increased when MSCs were cultured in nonadherent culture conditions. In addition, western blot and reverse transcription‑quantitative polymerase chain reaction analyses demonstrated that caspase‑3, ‑7 and ‑9 were involved in this process. The present study demonstrated that loss of culture adherence increases apoptosis of human MSCs. The present findings may provide new insight into the factors affecting MSC survival after transplantation.
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1 

Satija NK, Singh VK, Verma YK, Gupta P, Sharma S, Afrin F, Sharma M, Sharma P, Tripathi RP and Gurudutta GU: Mesenchymal stem cell-based therapy: A new paradigm in regenerative medicine. J Cell Mol Med. 13:4385–4402. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Novotny NM, Ray R, Markel TA, Crisostomo PR, Wang M, Wang Y and Meldrum DR: Stem cell therapy in myocardial repair and remodeling. J Am Coll Surg. 207:423–434. 2008. View Article : Google Scholar : PubMed/NCBI

3 

Deuse T, Peter C, Fedak PW, Doyle T, Reichenspurner H, Zimmermann WH, Eschenhagen T, Stein W, Wu JC, Robbins RC and Schrepfer S: Hepatocyte growth factor or vascular endothelial growth factor gene transfer maximizes mesenchymal stem cell-based myocardial salvage after acute myocardial infarction. Circulation. 120(11 Suppl): S247–S254. 2009. View Article : Google Scholar : PubMed/NCBI

4 

Rabani V, Shahsavani M, Gharavi M, Piryaei A, Azhdari Z and Baharvand H: Mesenchymal stem cell infusion therapy in a carbon tetrachloride-induced liver fibrosis model affects matrix metalloproteinase expression. Cell Biol Int. 34:601–605. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Burlacu A: Tracking the mesenchymal stem cell fate after transplantation into the infarcted myocardium. Curr Stem Cell Res Ther. 8:284–291. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Wei H, Li Z, Hu S, Chen X and Cong X: Apoptosis of mesenchymal stem cells induced by hydrogen peroxide concerns both endoplasmic reticulum stress and mitochondrial death pathway through regulation of caspases, p38 and JNK. J Cell Biochem. 111:967–978. 2010. View Article : Google Scholar : PubMed/NCBI

7 

Potier E, Ferreira E, Meunier A, Sedel L, Logeart-Avramoglou D and Petite H: Prolonged hypoxia concomitant with serum deprivation induces massive human mesenchymal stem cell death. Tissue Eng. 13:1325–1331. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Vunjak-Novakovic G and Scadden DT: Biomimetic platforms for human stem cell research. Cell Stem Cell. 8:252–261. 2011. View Article : Google Scholar : PubMed/NCBI

9 

Deng B, Deng W, Xiao P, Zeng K, Zhang S, Zhang H, Deng DY and Yang Y: Nonadherent culture method downregulates stem cell antigen-1 expression in mouse bone marrow mesenchymal stem cells. Exp Ther Med. 10:31–36. 2015.PubMed/NCBI

10 

Qian H, Le Blanc K and Sigvardsson M: Primary mesenchymal stem and progenitor cells from bone marrow lack expression of CD44 protein. J Biol Chem. 287:25795–25807. 2012. View Article : Google Scholar : PubMed/NCBI

11 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

12 

Schneider CA, Rasband WS and Eliceiri KW: NIH image to imageJ: 25 years of image analysis. Nat Methods. 9:671–675. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Dominici M, Le Blanc K, Mueller I, Slaper-Cortenbach I, Marini F, Krause D, Deans R, Keating A, Prockop Dj and Horwitz E: Minimal criteria for defining multipotent mesenchymal stromal cells. The International society for cellular therapy position statement. Cytotherapy. 8:315–317. 2006. View Article : Google Scholar : PubMed/NCBI

14 

Sheikh AY, Huber BC, Narsinh KH, Spin JM, van der Bogt K, de Almeida PE, Ransohoff KJ, Kraft DL, Fajardo G, Ardigo D, et al: In vivo functional and transcriptional profiling of bone marrow stem cells after transplantation into ischemic myocardium. Arterioscler Thromb Vasc Biol. 32:92–102. 2012. View Article : Google Scholar : PubMed/NCBI

15 

Baksh D, Davies JE and Zandstra PW: Adult human bone marrow-derived mesenchymal progenitor cells are capable of adhesion-independent survival and expansion. Exp Hematol. 31:723–732. 2003. View Article : Google Scholar : PubMed/NCBI

16 

Baksh D, Zandstra PW and Davies JE: A non-contact suspension culture approach to the culture of osteogenic cells derived from a CD49elow subpopulation of human bone marrow-derived cells. Biotechnol Bioeng. 98:1195–1208. 2007. View Article : Google Scholar : PubMed/NCBI

17 

Parrish AB, Freel CD and Kornbluth S: Cellular mechanisms controlling caspase activation and function. Cold Spring Harb Perspect Biol. 5:pii: a0086722013. View Article : Google Scholar

18 

Berghe T Vanden, Van Loo G, Saelens X, Van Gurp M, Brouckaert G, Kalai M, Declercq W and Vandenabeele P: Differential signaling to apoptotic and necrotic cell death by Fas-associated death domain protein FADD. J Biol Chem. 279:7925–7933. 2004. View Article : Google Scholar : PubMed/NCBI

19 

Rowinsky EK: Targeted induction of apoptosis in cancer management: The emerging role of tumor necrosis factor-related apoptosis-inducing ligand receptor activating agents. J Clin Oncol. 23:9394–9407. 2005. View Article : Google Scholar : PubMed/NCBI

20 

Philchenkov A, Zavelevich M, Kroczak TJ and Los M: Caspases and cancer: Mechanisms of inactivation and new treatment modalities. Exp Oncol. 26:82–97. 2004.PubMed/NCBI

21 

Deschepper M, Oudina K, David B, Myrtil V, Collet C, Bensidhoum M, Logeart-Avramoglou D and Petite H: Survival and function of mesenchymal stem cells (MSCs) depend on glucose to overcome exposure to long-term, severe and continuous hypoxia. J Cell Mol Med. 15:1505–1514. 2011. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Deng B, Jiang H, Zeng K, Liang Y, Wu Y and Yang Y: Removal from adherent culture contributes to apoptosis in human bone marrow mesenchymal stem cells. Mol Med Rep 15: 3499-3506, 2017.
APA
Deng, B., Jiang, H., Zeng, K., Liang, Y., Wu, Y., & Yang, Y. (2017). Removal from adherent culture contributes to apoptosis in human bone marrow mesenchymal stem cells. Molecular Medicine Reports, 15, 3499-3506. https://doi.org/10.3892/mmr.2017.6440
MLA
Deng, B., Jiang, H., Zeng, K., Liang, Y., Wu, Y., Yang, Y."Removal from adherent culture contributes to apoptosis in human bone marrow mesenchymal stem cells". Molecular Medicine Reports 15.6 (2017): 3499-3506.
Chicago
Deng, B., Jiang, H., Zeng, K., Liang, Y., Wu, Y., Yang, Y."Removal from adherent culture contributes to apoptosis in human bone marrow mesenchymal stem cells". Molecular Medicine Reports 15, no. 6 (2017): 3499-3506. https://doi.org/10.3892/mmr.2017.6440
Copy and paste a formatted citation
x
Spandidos Publications style
Deng B, Jiang H, Zeng K, Liang Y, Wu Y and Yang Y: Removal from adherent culture contributes to apoptosis in human bone marrow mesenchymal stem cells. Mol Med Rep 15: 3499-3506, 2017.
APA
Deng, B., Jiang, H., Zeng, K., Liang, Y., Wu, Y., & Yang, Y. (2017). Removal from adherent culture contributes to apoptosis in human bone marrow mesenchymal stem cells. Molecular Medicine Reports, 15, 3499-3506. https://doi.org/10.3892/mmr.2017.6440
MLA
Deng, B., Jiang, H., Zeng, K., Liang, Y., Wu, Y., Yang, Y."Removal from adherent culture contributes to apoptosis in human bone marrow mesenchymal stem cells". Molecular Medicine Reports 15.6 (2017): 3499-3506.
Chicago
Deng, B., Jiang, H., Zeng, K., Liang, Y., Wu, Y., Yang, Y."Removal from adherent culture contributes to apoptosis in human bone marrow mesenchymal stem cells". Molecular Medicine Reports 15, no. 6 (2017): 3499-3506. https://doi.org/10.3892/mmr.2017.6440
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