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Surfactant protein A is expressed in the central nervous system of rats with experimental autoimmune encephalomyelitis, and suppresses inflammation in human astrocytes and microglia

  • Authors:
    • Xue Yang
    • Jun Yan
    • Juan Feng
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, Shengjing Hospital, Affiliated Hospital of China Medical University. Shenyang, Liaoning 110004, P.R. China
    Copyright: © Yang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3555-3565
    |
    Published online on: April 7, 2017
       https://doi.org/10.3892/mmr.2017.6441
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Abstract

The collectin surfactant protein‑A (SP‑A), a potent host defense molecule, is well recognized for its role in the maintenance of pulmonary homeostasis and the modulation of inflammatory responses. While previous studies have detected SP‑A in numerous extrapulmonary tissues, there is still a lack of information regarding its expression in central nervous system (CNS) and potential effects in neuroinflammatory diseases, such as multiple sclerosis (MS). The present study used experimental autoimmune encephalomyelitis (EAE), the most commonly used animal model of MS, to investigate the expression of SP‑A in the CNS at different stages of disease progression. In addition, in vitro experiments with lipopolysaccharide (LPS)‑stimulated human astrocytes and microglia were performed to investigate the potential role of SP‑A in the modulation of CNS inflammatory responses. The results of the present study demonstrated widespread distribution of SP‑A in the rat CNS, and also identified specific expression patterns of SP‑A at different stages of EAE. In vitro, the current study revealed that treatment of human astrocytes and microglia with LPS promoted SP‑A expression in a dose‑dependent manner. Furthermore, exogenous SP‑A protein significantly decreased Toll‑like receptor 4 and nuclear factor‑κB expression, and reduced interleukin‑1β and tumor necrosis factor‑α levels. The results of the current study indicate a potential role for SP‑A in the modulation of CNS inflammatory responses.
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Copy and paste a formatted citation
Spandidos Publications style
Yang X, Yan J and Feng J: Surfactant protein A is expressed in the central nervous system of rats with experimental autoimmune encephalomyelitis, and suppresses inflammation in human astrocytes and microglia. Mol Med Rep 15: 3555-3565, 2017.
APA
Yang, X., Yan, J., & Feng, J. (2017). Surfactant protein A is expressed in the central nervous system of rats with experimental autoimmune encephalomyelitis, and suppresses inflammation in human astrocytes and microglia. Molecular Medicine Reports, 15, 3555-3565. https://doi.org/10.3892/mmr.2017.6441
MLA
Yang, X., Yan, J., Feng, J."Surfactant protein A is expressed in the central nervous system of rats with experimental autoimmune encephalomyelitis, and suppresses inflammation in human astrocytes and microglia". Molecular Medicine Reports 15.6 (2017): 3555-3565.
Chicago
Yang, X., Yan, J., Feng, J."Surfactant protein A is expressed in the central nervous system of rats with experimental autoimmune encephalomyelitis, and suppresses inflammation in human astrocytes and microglia". Molecular Medicine Reports 15, no. 6 (2017): 3555-3565. https://doi.org/10.3892/mmr.2017.6441
Copy and paste a formatted citation
x
Spandidos Publications style
Yang X, Yan J and Feng J: Surfactant protein A is expressed in the central nervous system of rats with experimental autoimmune encephalomyelitis, and suppresses inflammation in human astrocytes and microglia. Mol Med Rep 15: 3555-3565, 2017.
APA
Yang, X., Yan, J., & Feng, J. (2017). Surfactant protein A is expressed in the central nervous system of rats with experimental autoimmune encephalomyelitis, and suppresses inflammation in human astrocytes and microglia. Molecular Medicine Reports, 15, 3555-3565. https://doi.org/10.3892/mmr.2017.6441
MLA
Yang, X., Yan, J., Feng, J."Surfactant protein A is expressed in the central nervous system of rats with experimental autoimmune encephalomyelitis, and suppresses inflammation in human astrocytes and microglia". Molecular Medicine Reports 15.6 (2017): 3555-3565.
Chicago
Yang, X., Yan, J., Feng, J."Surfactant protein A is expressed in the central nervous system of rats with experimental autoimmune encephalomyelitis, and suppresses inflammation in human astrocytes and microglia". Molecular Medicine Reports 15, no. 6 (2017): 3555-3565. https://doi.org/10.3892/mmr.2017.6441
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