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Article

Tetrahydropalmatine inhibits lipid accumulation through AMPK signaling pathway in 3T3‑L1 adipocytes

  • Authors:
    • Guang‑Chun Piao
    • Guan‑Cheng Liu
    • Xue‑Jun Jin
    • Dan Jin
    • Hai‑Dan Yuan
  • View Affiliations / Copyright

    Affiliations: Key Laboratory for Natural Resources of Changbai Mountain and Functional Molecules, Ministry of Education, College of Pharmacy, Yanbian University, Yanji, Jilin 133002, P.R. China
  • Pages: 3912-3918
    |
    Published online on: April 12, 2017
       https://doi.org/10.3892/mmr.2017.6473
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Abstract

Tetrahydropalmatine (THP), one of the active components of Rhizoma corydalis, has been reported to exert several pharmacological effects, including anti‑inflammatory, anti‑tumor and analgesic activities. However, its effect on obesity and the underlying molecular mechanisms that may be involved have not yet been elucidated. In the present study, the inhibitory effects of THP on the adipogenesis in 3T3‑L1 adipocytes was examined using hstology, western blotting and RT‑qPCR. THP was identified to significantly suppress lipid accumulation in 3T3‑L1 cells and it inhibited pre‑adipocyte differentiation in a concentration‑dependent manner, as evidenced by the reduced formation of lipid droplets and decreased triglyceride levels and glycerol‑3‑phosphate dehydrogenase activity. THP downregulated the adipogenesis‑associated protein and gene expressions of sterol regulatory element‑binding protein 1, fatty acid synthase, stearoyl‑CoA desaturase 1, peroxisome proliferator activated receptor γ and CCAAT/enhancer binding protein‑α in a concentration‑dependent manner. In addition, it reduced adipocyte fatty acid binding protein and glycerol‑3‑phosphate acyltransferase gene expression in a concentration‑dependent manner. Conversely, THP increased the mRNA expression of carnitine palmitoyltransferase 1 in a concentration‑dependent manner. Furthermore, THP increased AMP‑activated protein kinase (AMPK) and acetyl‑CoA carboxylase phosphorylation in a concentration‑dependent manner. These results suggested that anti‑adipogenic activity of TPH may be mediated via the AMPK pathway in 3T3‑L1 cells.
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Copy and paste a formatted citation
Spandidos Publications style
Piao GC, Liu GC, Jin XJ, Jin D and Yuan HD: Tetrahydropalmatine inhibits lipid accumulation through AMPK signaling pathway in 3T3‑L1 adipocytes. Mol Med Rep 15: 3912-3918, 2017.
APA
Piao, G., Liu, G., Jin, X., Jin, D., & Yuan, H. (2017). Tetrahydropalmatine inhibits lipid accumulation through AMPK signaling pathway in 3T3‑L1 adipocytes. Molecular Medicine Reports, 15, 3912-3918. https://doi.org/10.3892/mmr.2017.6473
MLA
Piao, G., Liu, G., Jin, X., Jin, D., Yuan, H."Tetrahydropalmatine inhibits lipid accumulation through AMPK signaling pathway in 3T3‑L1 adipocytes". Molecular Medicine Reports 15.6 (2017): 3912-3918.
Chicago
Piao, G., Liu, G., Jin, X., Jin, D., Yuan, H."Tetrahydropalmatine inhibits lipid accumulation through AMPK signaling pathway in 3T3‑L1 adipocytes". Molecular Medicine Reports 15, no. 6 (2017): 3912-3918. https://doi.org/10.3892/mmr.2017.6473
Copy and paste a formatted citation
x
Spandidos Publications style
Piao GC, Liu GC, Jin XJ, Jin D and Yuan HD: Tetrahydropalmatine inhibits lipid accumulation through AMPK signaling pathway in 3T3‑L1 adipocytes. Mol Med Rep 15: 3912-3918, 2017.
APA
Piao, G., Liu, G., Jin, X., Jin, D., & Yuan, H. (2017). Tetrahydropalmatine inhibits lipid accumulation through AMPK signaling pathway in 3T3‑L1 adipocytes. Molecular Medicine Reports, 15, 3912-3918. https://doi.org/10.3892/mmr.2017.6473
MLA
Piao, G., Liu, G., Jin, X., Jin, D., Yuan, H."Tetrahydropalmatine inhibits lipid accumulation through AMPK signaling pathway in 3T3‑L1 adipocytes". Molecular Medicine Reports 15.6 (2017): 3912-3918.
Chicago
Piao, G., Liu, G., Jin, X., Jin, D., Yuan, H."Tetrahydropalmatine inhibits lipid accumulation through AMPK signaling pathway in 3T3‑L1 adipocytes". Molecular Medicine Reports 15, no. 6 (2017): 3912-3918. https://doi.org/10.3892/mmr.2017.6473
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