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Anti‑inflammatory effects of oxymatrine on rheumatoid arthritis in rats via regulating the imbalance between Treg and Th17 cells

  • Authors:
    • Ailing Ma
    • Yongya Yang
    • Qiuyang Wang
    • Yin Wang
    • Jing Wen
    • Yanli Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, The General Hospital of Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China, Department of Pathogen Biology and Immunology, School of Basic Medical Sciences, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China, Department of Laboratory, the People's Hospital of Yan'an, Yan'an, Shanxi 71600, P.R. China, Ningxia Key Laboratory of Cerebrocranial Diseases, Ningxia Medical University, Yinchuan, Ningxia 750004, P.R. China
    Copyright: © Ma et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3615-3622
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    Published online on: April 19, 2017
       https://doi.org/10.3892/mmr.2017.6484
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Abstract

Oxymatrine (OMT), a monosomic alkaloid extracted from the Chinese herb, Sophora flavescens Ait, has long been used as a traditional Chinese medicine for the treatment of inflammatory diseases. The aim of the present study was to investigate the potential anti‑inflammatory effect of OMT, and its modulation on imbalance between regulatory T (Treg) cells and T helper (Th) 17 cells in rats with collagen‑induced arthritis (CIA). Sprague‑Dawley rats were immunized with type II collagen and following a second collagen immunization, the rats were treated with OMT or dexamethasone (DXM) intraperitoneally once a day for 43 days. Paw swelling, arthritic score and joint histopathology were evaluated. The Treg/Th17‑mediated autoreactive response was assessed by determining serum levels of inflammatory response cytokines, including tumor necrosis factor (TNF)‑α and interleukin (IL)‑17, using an enzyme‑linked immunosorbent assay. The mRNA levels of forkhead box P3 (FOXP3) and retinoic acid‑related orphan receptor (ROR)γt in spleen cells stimulated with type II collagen were determined using reverse transcription‑quantitative polymerase chain reaction analysis. In addition, the protein expression levels of FOXP3 and RORγt were measured using western blot analysis. The results showed that OMT treatment significantly reduced the severity of CIA, markedly abrogating paw swelling, arthritic scores and synovial hyperplasia, and the increased loss in body weight. OMT significantly reduced the production of TNF‑α and IL‑17A, upregulated FOXP3 and downregulated RORγt in rats with CIA. In conclusion, the present study demonstrated that OMT exhibited a protective effect on rheumatoid arthritis (RA) through the inhibition of inflammation and regulation of Treg/Th17 in the CIA rats, suggesting that OMT may be used as an immune suppressive and cartilage protective medicine in human RA.
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Copy and paste a formatted citation
Spandidos Publications style
Ma A, Yang Y, Wang Q, Wang Y, Wen J and Zhang Y: Anti‑inflammatory effects of oxymatrine on rheumatoid arthritis in rats via regulating the imbalance between Treg and Th17 cells. Mol Med Rep 15: 3615-3622, 2017.
APA
Ma, A., Yang, Y., Wang, Q., Wang, Y., Wen, J., & Zhang, Y. (2017). Anti‑inflammatory effects of oxymatrine on rheumatoid arthritis in rats via regulating the imbalance between Treg and Th17 cells. Molecular Medicine Reports, 15, 3615-3622. https://doi.org/10.3892/mmr.2017.6484
MLA
Ma, A., Yang, Y., Wang, Q., Wang, Y., Wen, J., Zhang, Y."Anti‑inflammatory effects of oxymatrine on rheumatoid arthritis in rats via regulating the imbalance between Treg and Th17 cells". Molecular Medicine Reports 15.6 (2017): 3615-3622.
Chicago
Ma, A., Yang, Y., Wang, Q., Wang, Y., Wen, J., Zhang, Y."Anti‑inflammatory effects of oxymatrine on rheumatoid arthritis in rats via regulating the imbalance between Treg and Th17 cells". Molecular Medicine Reports 15, no. 6 (2017): 3615-3622. https://doi.org/10.3892/mmr.2017.6484
Copy and paste a formatted citation
x
Spandidos Publications style
Ma A, Yang Y, Wang Q, Wang Y, Wen J and Zhang Y: Anti‑inflammatory effects of oxymatrine on rheumatoid arthritis in rats via regulating the imbalance between Treg and Th17 cells. Mol Med Rep 15: 3615-3622, 2017.
APA
Ma, A., Yang, Y., Wang, Q., Wang, Y., Wen, J., & Zhang, Y. (2017). Anti‑inflammatory effects of oxymatrine on rheumatoid arthritis in rats via regulating the imbalance between Treg and Th17 cells. Molecular Medicine Reports, 15, 3615-3622. https://doi.org/10.3892/mmr.2017.6484
MLA
Ma, A., Yang, Y., Wang, Q., Wang, Y., Wen, J., Zhang, Y."Anti‑inflammatory effects of oxymatrine on rheumatoid arthritis in rats via regulating the imbalance between Treg and Th17 cells". Molecular Medicine Reports 15.6 (2017): 3615-3622.
Chicago
Ma, A., Yang, Y., Wang, Q., Wang, Y., Wen, J., Zhang, Y."Anti‑inflammatory effects of oxymatrine on rheumatoid arthritis in rats via regulating the imbalance between Treg and Th17 cells". Molecular Medicine Reports 15, no. 6 (2017): 3615-3622. https://doi.org/10.3892/mmr.2017.6484
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