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Article

MicroRNA‑336 directly targets Sox‑2 in osteosarcoma to inhibit tumorigenesis

Retraction in: /10.3892/mmr.2024.13264
  • Authors:
    • Yong Cao
    • Tianding Wu
    • Dongzhe Li
    • Jianzhong Hu
    • Hongbin Lu
  • View Affiliations / Copyright

    Affiliations: Department of Spine Surgery, Xiangya Hospital, Central South University, Changsha, Hunan 410008, P.R. China
  • Pages: 4217-4224
    |
    Published online on: April 21, 2017
       https://doi.org/10.3892/mmr.2017.6493
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Abstract

Previous evidence has suggested that microRNAs (miRNAs or miRs), which belong to a class of non‑coding RNAs, shape cellular processes by regulating gene expression. Abnormal expression of miRNAs has been associated with tumorigenesis in multiple cancers. However, the function of miR‑336 in osteosarcoma (OS) remains unknown. The experimental procedures used in the present study included flow cytometry, reverse transcription‑quantitative polymerase chain reaction, luciferase reporter assay, invasion assay, western blot analysis and in vivo implantation. The results of the present study demonstrated that miR‑336 may serve as a tumor suppressor in OS. Downregulation of miR‑336 was observed in human OS specimens as well as OS cell lines. In addition, a significant negative correlation between sex determining region Y‑box 2 (Sox‑2) expression and miR‑336 was demonstrated. miR‑336 was confirmed to target the 3'‑untranslated region of Sox‑2 to inhibit proliferation, migration and invasion of OS cells. Consistently, restoration of Sox‑2 expression counteracted the effect of miR‑336, and recovered the tumorigenic potential of OS cells. The present study established a novel association between miR‑336 and Sox‑2 in OS. This relationship between miR‑336 and Sox‑2 may lead to improved knowledge concerning OS progression and sheds light on potential novel therapeutic interventions for OS treatment.
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1 

Ottaviani G and Jaffe N: The epidemiology of osteosarcoma. Cancer Treat Res. 152:3–13. 2009. View Article : Google Scholar

2 

Morrow JJ and Khanna C: Osteosarcoma genetics and epigenetics: Emerging biology and candidate therapies. Crit Rev Oncog. 20:173–197. 2015. View Article : Google Scholar :

3 

Admassi D: Osteosarcoma of medial cuniform bone. Ethiop Med J. 47:305–308. 2009.

4 

Miao J, Wu S, Peng Z, Tania M and Zhang C: MicroRNAs in osteosarcoma: Diagnostic and therapeutic aspects. Tumour Biol. 34:2093–2098. 2013. View Article : Google Scholar

5 

Osborne TS and Khanna C: A review of the association between osteosarcoma metastasis and protein translation. J Comp Pathol. 146:132–142. 2012. View Article : Google Scholar :

6 

Akiyama T, Dass CR and Choong PF: Novel therapeutic strategy for osteosarcoma targeting osteoclast differentiation, bone-resorbing activity, and apoptosis pathway. Mol Cancer Ther. 7:3461–3469. 2008. View Article : Google Scholar

7 

Miska EA: How microRNAs control cell division, differentiation and death. Curr Opin Genet Dev. 15:563–568. 2005. View Article : Google Scholar

8 

Cai Y, Yu X, Hu S and Yu J: A brief review on the mechanisms of miRNA regulation. Genomics Proteomics Bioinformatics. 7:147–154. 2009. View Article : Google Scholar

9 

Bartel DP: MicroRNAs: Target recognition and regulatory functions. Cell. 136:215–233. 2009. View Article : Google Scholar :

10 

Feng B, Zhang K, Wang R and Chen L: Non-small-cell lung cancer and miRNAs: Novel biomarkers and promising tools for treatment. Clin Sci (Lond). 128:619–634. 2015. View Article : Google Scholar

11 

van Rooij E: The art of microRNA research. Circ Res. 108:219–234. 2011. View Article : Google Scholar

12 

Liu X, Zhang J, Xie B, Li H, Shen J and Chen J: MicroRNA-200 family profile: A promising ancillary tool for accurate cancer diagnosis. Am J Ther. 23:e388–e397. 2016. View Article : Google Scholar :

13 

Oom AL, Humphries BA and Yang C: MicroRNAs: Novel players in cancer diagnosis and therapies. Biomed Res Int. 2014:9594612014. View Article : Google Scholar :

14 

He C, Xiong J, Xu X, et al: Functional elucidation of MiR-34 in osteosarcoma cells and primary tumor samples. Biochem Biophys Res Commun. 388:35–40. 2009. View Article : Google Scholar

15 

Zhao H, Ma B, Wang Y, et al: miR-34a inhibits the metastasis of osteosarcoma cells by repressing the expression of CD44. Oncol Rep. 29:1027–1036. 2013.

16 

Ziyan W, Shuhua Y, Xiufang W and Xiaoyun L: MicroRNA-21 is involved in osteosarcoma cell invasion and migration. Med Oncol. 28:1469–1474. 2011. View Article : Google Scholar

17 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) Method. Methods. 25:402–408. 2001. View Article : Google Scholar

18 

Yang C, Hou C, Zhang H, Wang D, Ma Y, Zhang Y, Xu X, Bi Z and Geng S: miR-126 functions as a tumor suppressor in osteosarcoma by targeting Sox2. Int J Mol Sci. 15:423–437. 2013. View Article : Google Scholar :

19 

Wong N and Wang X: miRDB: An online resource for microRNA target prediction and functional annotations. Nucleic Acids Res. 43:D146–D152. 2015. View Article : Google Scholar

20 

Lewis BP, Burge CB and Bartel DP: Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets. Cell. 120:15–20. 2005. View Article : Google Scholar

21 

Li J, Du L, Yang Y, Wang C, Liu H, Wang L, Zhang X, Li W, Zheng G and Dong Z: MiR-429 is an independent prognostic factor in colorectal cancer and exerts its anti-apoptotic function by targeting SOX2. Cancer Lett. 329:84–90. 2013. View Article : Google Scholar

22 

Leonardo TR, Schultheisz HL, Loring JF and Laurent LC: The functions of microRNAs in pluripotency and reprogramming. Nat Cell Biol. 14:1114–1121. 2012. View Article : Google Scholar

23 

Croset M, Santini D, Iuliani M, et al: MicroRNAs and bone metastasis: a new challenge. Molecules. 19:10115–10128. 2014. View Article : Google Scholar

24 

Sukata T, Sumida K, Kushida M, et al: Circulating microRNAs, possible indicators of progress of rat hepatocarcinogenesis from early stages. Toxicol Lett. 200:46–52. 2011. View Article : Google Scholar

25 

Truettner JS, Katyshev V, Esen-Bilgin N, Dietrich WD and Dore-Duffy P: Hypoxia alters MicroRNA expression in rat cortical pericytes. Microrna. 2:32–44. 2013. View Article : Google Scholar :

26 

Basu-Roy U, Seo E, Ramanathapuram L, Rapp TB, Perry JA, Orkin SH, Mansukhani A and Basilico C: Sox2 maintains self renewal of tumor-initiating cells in osteosarcomas. Oncogene. 31:2270–2282. 2012. View Article : Google Scholar

27 

Basu-Roy U, Ambrosetti D, Favaro R, Nicolis SK, Mansukhani A and Basilico C: The transcription factor Sox2 is required for osteoblast self-renewal. Cell Death Differ. 17:1345–1353. 2010. View Article : Google Scholar :

28 

Mansukhani A, Ambrosetti D, Holmes G, Cornivelli L and Basilico C: Sox2 induction by FGF and FGFR2 activating mutations inhibits Wnt signaling and osteoblast differentiation. J Cell Biol. 168:1065–1076. 2005. View Article : Google Scholar :

29 

Zhang Y, Yeh LK, Zhang S, Call M, Yuan Y, Yasunaga M, Kao WW and Liu CY: Wnt/β-catenin signaling modulates corneal epithelium stratification via inhibition of Bmp4 during mouse development. Development. 142:3383–3393. 2015. View Article : Google Scholar :

30 

Bass AJ, Watanabe H, Mermel CH, Yu S, Perner S, Verhaak RG, Kim SY, Wardwell L, Tamayo P, Gat-Viks I, et al: SOX2 is an amplified lineage-survival oncogene in lung and esophageal squamous cell carcinomas. Nat Genet. 41:1238–1242. 2009. View Article : Google Scholar :

31 

Sun C, Sun L, Li Y, Kang X, Zhang S and Liu Y: Sox2 expression predicts poor survival of hepatocellular carcinoma patients and it promotes liver cancer cell invasion by activating Slug. Med Oncol. 30:5032013. View Article : Google Scholar

32 

Deng Z, Du WW, Fang L, Shan SW, Qian J, Lin J, Qian W, Ma J, Rutnam ZJ and Yang BB: The intermediate filament vimentin mediates microRNA miR-378 function in cellular self-renewal by regulating the expression of the Sox2 transcription factor. J Biol Chem. 288:319–331. 2013. View Article : Google Scholar

33 

Zhang Y, Eades G, Yao Y, Li Q and Zhou Q: Estrogen receptor α signaling regulates breast tumor-initiating cells by down-regulating miR-140 which targets the transcription factor SOX2. J Biol Chem. 287:41514–41522. 2012. View Article : Google Scholar :

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Copy and paste a formatted citation
Spandidos Publications style
Cao Y, Wu T, Li D, Hu J and Lu H: MicroRNA‑336 directly targets Sox‑2 in osteosarcoma to inhibit tumorigenesis Retraction in /10.3892/mmr.2024.13264. Mol Med Rep 15: 4217-4224, 2017.
APA
Cao, Y., Wu, T., Li, D., Hu, J., & Lu, H. (2017). MicroRNA‑336 directly targets Sox‑2 in osteosarcoma to inhibit tumorigenesis Retraction in /10.3892/mmr.2024.13264. Molecular Medicine Reports, 15, 4217-4224. https://doi.org/10.3892/mmr.2017.6493
MLA
Cao, Y., Wu, T., Li, D., Hu, J., Lu, H."MicroRNA‑336 directly targets Sox‑2 in osteosarcoma to inhibit tumorigenesis Retraction in /10.3892/mmr.2024.13264". Molecular Medicine Reports 15.6 (2017): 4217-4224.
Chicago
Cao, Y., Wu, T., Li, D., Hu, J., Lu, H."MicroRNA‑336 directly targets Sox‑2 in osteosarcoma to inhibit tumorigenesis Retraction in /10.3892/mmr.2024.13264". Molecular Medicine Reports 15, no. 6 (2017): 4217-4224. https://doi.org/10.3892/mmr.2017.6493
Copy and paste a formatted citation
x
Spandidos Publications style
Cao Y, Wu T, Li D, Hu J and Lu H: MicroRNA‑336 directly targets Sox‑2 in osteosarcoma to inhibit tumorigenesis Retraction in /10.3892/mmr.2024.13264. Mol Med Rep 15: 4217-4224, 2017.
APA
Cao, Y., Wu, T., Li, D., Hu, J., & Lu, H. (2017). MicroRNA‑336 directly targets Sox‑2 in osteosarcoma to inhibit tumorigenesis Retraction in /10.3892/mmr.2024.13264. Molecular Medicine Reports, 15, 4217-4224. https://doi.org/10.3892/mmr.2017.6493
MLA
Cao, Y., Wu, T., Li, D., Hu, J., Lu, H."MicroRNA‑336 directly targets Sox‑2 in osteosarcoma to inhibit tumorigenesis Retraction in /10.3892/mmr.2024.13264". Molecular Medicine Reports 15.6 (2017): 4217-4224.
Chicago
Cao, Y., Wu, T., Li, D., Hu, J., Lu, H."MicroRNA‑336 directly targets Sox‑2 in osteosarcoma to inhibit tumorigenesis Retraction in /10.3892/mmr.2024.13264". Molecular Medicine Reports 15, no. 6 (2017): 4217-4224. https://doi.org/10.3892/mmr.2017.6493
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