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Association between high mobility group box‑1 protein expression and cell death in acute pancreatitis

  • Authors:
    • Enjun Gao
    • Yanfeng Jiang
    • Zhituo Li
    • Dongbo Xue
    • Weihui Zhang
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China
    Copyright: © Gao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4021-4026
    |
    Published online on: April 21, 2017
       https://doi.org/10.3892/mmr.2017.6496
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Abstract

The present study used caerulein stimulation of AR42J rat pancreatic cells as an in vitro acute pancreatitis (AP) model to investigate proteins differentially expressed in apoptosis and necrosis. AR42J cells were stimulated with 10‑8mol/l caerulein and incubated for 24 h. Apoptosis and necrosis were detected using flow cytometry. The sorted Annexin V‑positive cells (apoptotic) and the Annexin V/propidium iodide double‑positive cells (necrotic) were analysed using proteomics. Results showed that numerous proteins were differentially expressed in these 2 groups. Functional enrichment analysis was performed on the differentially expressed genes using the Database for Annotation, Visualization and Integrated Discovery. High mobility group box‑1 protein (HMGB1) was specifically expressed in the necrosis group. Models of varying degrees of AP were established using caerulein at concentrations of 10‑9, 10‑8, 10‑7, 10‑6 and 10‑5 mol/l. The percentage of apoptotic and necrotic cells in each group was determined using flow cytometry. Protein expression levels of HMGB1 were detected by western blot analysis. The present study showed that as the concentration of caerulein increased, the percentage of necrotic cells and the protein expression levels of HMGB1 increased. HMGB1 is involved in many biological processes, including the chromosomal protein glycyl lysine isopeptide cross‑link. HMGB1 may be involved in the early stage of pancreatitis, potentially by inducing the development of cell death by necrosis. These results provide an experimental basis for clinical intervention in AP.
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1 

Petersen OH, Tepikin AV, Gerasimenko JV, Gerasimenko OV, Sutton R and Criddle DN: Fatty acids, alcohol and fatty acid ethylesters: Toxic Ca2+ signal generation and pancreatitis. Cell Calcium. 45:634–642. 2009. View Article : Google Scholar

2 

Keck T, Friebe V, Warshaw AL, Antoniu BA, Waneck G, Benz S, Hopt UT and Fernández-del-Castillo C: Pancreatic proteases in serum Leukocyte-endothelial adhesion and pancreatic microcirculatory failure. Pancreatology. 5:241–250. 2005. View Article : Google Scholar

3 

Leung PS and Chan YC: Role of oxidative stress in pancreatic inflammation. Antioxid Redox Signal. 11:135–165. 2009. View Article : Google Scholar

4 

Golstein P and Kroemer G: Cell death by necrosis: Towards a molecular definition. Trends Biochem Sci. 32:37–43. 2007. View Article : Google Scholar

5 

Rinderknecht H: Fatal pancreatitis, a consequence of excessive leukocyte stimulation? Int J Pancreatol. 3:105–112. 1988.

6 

Ziangirova GG and Antonova OV: The causes of necrobiosis and apoptosis of corneal epithelial cells during primary acquired keratoconus. Izv Akad Nauk Ser Biol. 5:517–521. 2002.(In Russian).

7 

Andersson R and Wang XD: Patterns of pancreatic cell death: Apoptosis versus oncosis. Pancreas. 17:281–288. 1998. View Article : Google Scholar

8 

Chu J, Ji H, Lu M, Li Z, Qiao X, Sun B, Zhang W and Xue D: Proteomic analysis of apoptotic and oncotic pancreatic acinar AR42J cells treated with caerulein. Mol Cell Biochem. 382:1–17. 2013. View Article : Google Scholar

9 

Bhatia M: Apoptosis versus necrosis in acute pancreatitis. Am J Physiol Gastrointest Liver Physiol. 286:G189–G196. 2004. View Article : Google Scholar

10 

Hori O, Brett J, Slattery T, Cao R, Zhang J, Chen JX, Nagashima M, Lundh ER, Vijay S, Nitecki D, et al: The receptor for advanced glycation end products (RAGE) is a cellular binding site for amphoterin. Mediation of neurite outgrowth and co-expression of rage and amphoterin in the developing nervous system. J Biol Chem. 270:25752–25761. 1995. View Article : Google Scholar

11 

Park JS, Svetkauskaite D, He Q, Kim JY, Strassheim D, Ishizaka A and Abraham E: Involvement of toll-like receptors 2 and 4 in cellular activation by high mobility group box 1 protein. J Biol Chem. 279:7370–7307. 2004. View Article : Google Scholar

12 

Urbonaviciute V, Fürnrohr BG, Meister S, Munoz L, Heyder P, De Marchis F, Bianchi ME, Kirschning C, Wagner H, Manfredi AA, et al: Induction of inflammatory and immune responses by HMGB1-nucleosome complexes: Implications for the pathogenesis of SLE. J Exp Med. 205:3007–3018. 2008. View Article : Google Scholar :

13 

Park JS, Gamboni-Robertson F, He Q, Svetkauskaite D, Kim JY, Strassheim D, Sohn JW, Yamada S, Maruyama I, Banerjee A, et al: High mobility group box 1 protein interacts with multiple Toll-like receptors. Am J Physiol Cell Physiol. 290:C917–C924. 2006. View Article : Google Scholar

14 

Goodwin GH, Sanders C and Johns EW: A new group of chromatinassociated proteins with a high content of acidic and basic amino acids. Eur J Biochem. 38:14–19. 1973. View Article : Google Scholar

15 

Wang H, Bloom O, Zhang M, Vishnubhakat JM, Ombrellino M, Che J, Frazier A, Yang H, Ivanova S, Borovikova L, et al: HMG-1 as a late mediator of endotoxin lethality in mice. Science. 285:248–251. 1999. View Article : Google Scholar

16 

Wang H, Yang H, Czura CJ, Sama AE and Tracey KJ: HMGB1 as a late mediator of lethal systemic inflammation. Am J Respir Crit Care Med. 164:1768–1773. 2001. View Article : Google Scholar

17 

Magna M and Pisetsky DS: The role of HMGB1 in the pathogenesis of inflammatory and autoimmune diseases. Mol Med. 20:138–146. 2014. View Article : Google Scholar :

18 

Singh A, Feng Y, Mahato N, Li J, Wu C and Gong J: Role of high-mobility group box 1 in patients with acute obstructive suppurative cholangitis-induced sepsis. J Inflamm Res. 8:71–77. 2015. View Article : Google Scholar :

19 

Yasuda T, Ueda T, Takeyama Y, Shinzeki M, Sawa H, Nakajima T, Ajiki T, Fujino Y, Suzuki Y and Kuroda Y: Significant increase of serum high-mobility group box chromosomal protein 1 levels in patients with severe acute pancreatitis. Pancreas. 33:359–363. 2006. View Article : Google Scholar

20 

Sawa H, Ueda T, Takeyama Y, Yasuda T, Shinzeki M, Nakajima T and Kuroda Y: Blockade of high mobility group box-1 protein attenuates experimental severe acute pancreatitis. World J Gastroenterol. 12:7666–7670. 2006. View Article : Google Scholar :

21 

Scaffidi P, Misteli T and Bianchi ME: Release of chromatin protein HMGB1 by necrotic cells triggers inflammation. Nature. 418:191–195. 2002. View Article : Google Scholar

22 

Yu C, Huang L, Li X, Zhu H, Li Z and Yu X: Spatial and temporal differences of HMGB1 expression in the pancreas of rats with acute pancreatitis. Int J Clin Exp Pathol. 8:6928–6935. 2015.

23 

Biscetti F, Ghirlanda G and Flex A: Therapeutic potential of high mobility group box-1 in ischemic injury and tissue regeneration. Curr Vasc Pharmacol. 9:677–681. 2011. View Article : Google Scholar

24 

Yasuda T, Ueda T, Shinzeki M, Sawa H, Nakajima T, Takeyama Y and Kuroda Y: Increase of high-mobility group box chromosomal protein 1 in blood and injured organs in experimental severe acute pancreatitis. Pancreas. 34:487–488. 2007. View Article : Google Scholar

25 

Sah RP and Saluja A: Molecular mechanisms of pancreatic injury. Curr Opin Gastroenterol. 27:444–451. 2011. View Article : Google Scholar :

26 

Bünger R, Mallet RT and Hartman DA: Pyruvate-enhanced phosphorylation potential and inotropism in normoxic and postischemic isolated working heart. Near-complete prevention of reperfusion contractile failure. Eur J Biochem. 180:221–233. 1989. View Article : Google Scholar

27 

Cicalese L, Lee K, Schraut W, Watkins S, Borle A and Stanko R: Pyruvate prevents ischemia-reperfusion mucosal injury of rat small intestine. Am J Surg. 171:97–101. 1996. View Article : Google Scholar

28 

Sileri P, Schena S, Morini S, Rastellini C, Pham S, Benedetti E and Cicalese L: Pyruvate inhibits hepatic ischemia-reperfusion injury in rats. Transplantation. 72:27–30. 2001. View Article : Google Scholar

29 

Miyaji T, Hu X, Yuen PS, Muramatsu Y, Iyer S, Hewitt SM and Star RA: Ethyl pyruvate decreases sepsis-induced acute renal failure and multiple organ damage in aged mice. Kidney Int. 64:1620–1631. 2003. View Article : Google Scholar

30 

Satoh A, Shimosegawa T, Fujita M, Kimura K, Masamune A, Koizumi M and Toyota T: Inhibition of nuclear factor-kappaB activation improves the survival of rats with taurocholate pancreatitis. Gut. 44:253–258. 1999. View Article : Google Scholar :

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Copy and paste a formatted citation
Spandidos Publications style
Gao E, Jiang Y, Li Z, Xue D and Zhang W: Association between high mobility group box‑1 protein expression and cell death in acute pancreatitis. Mol Med Rep 15: 4021-4026, 2017.
APA
Gao, E., Jiang, Y., Li, Z., Xue, D., & Zhang, W. (2017). Association between high mobility group box‑1 protein expression and cell death in acute pancreatitis. Molecular Medicine Reports, 15, 4021-4026. https://doi.org/10.3892/mmr.2017.6496
MLA
Gao, E., Jiang, Y., Li, Z., Xue, D., Zhang, W."Association between high mobility group box‑1 protein expression and cell death in acute pancreatitis". Molecular Medicine Reports 15.6 (2017): 4021-4026.
Chicago
Gao, E., Jiang, Y., Li, Z., Xue, D., Zhang, W."Association between high mobility group box‑1 protein expression and cell death in acute pancreatitis". Molecular Medicine Reports 15, no. 6 (2017): 4021-4026. https://doi.org/10.3892/mmr.2017.6496
Copy and paste a formatted citation
x
Spandidos Publications style
Gao E, Jiang Y, Li Z, Xue D and Zhang W: Association between high mobility group box‑1 protein expression and cell death in acute pancreatitis. Mol Med Rep 15: 4021-4026, 2017.
APA
Gao, E., Jiang, Y., Li, Z., Xue, D., & Zhang, W. (2017). Association between high mobility group box‑1 protein expression and cell death in acute pancreatitis. Molecular Medicine Reports, 15, 4021-4026. https://doi.org/10.3892/mmr.2017.6496
MLA
Gao, E., Jiang, Y., Li, Z., Xue, D., Zhang, W."Association between high mobility group box‑1 protein expression and cell death in acute pancreatitis". Molecular Medicine Reports 15.6 (2017): 4021-4026.
Chicago
Gao, E., Jiang, Y., Li, Z., Xue, D., Zhang, W."Association between high mobility group box‑1 protein expression and cell death in acute pancreatitis". Molecular Medicine Reports 15, no. 6 (2017): 4021-4026. https://doi.org/10.3892/mmr.2017.6496
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