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Article

Expression of programmed cell death1 in T follicular helper cells is regulated by prostaglandin E2 secreted by HBV-infected HepG2.2.1.5 cells

  • Authors:
    • Zhefeng Sui
    • Ying Shi
    • Zhiling Gao
    • Deguang Yang
    • Zhihao Wang
  • View Affiliations / Copyright

    Affiliations: Department of Nursing, Hulunbeier Vocational Technical College, Hulunbuir, Inner Mongolia 021000, P.R. China, Department of Hepatology, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China, Department of Pharmacy, Baicheng Medical College, Baicheng, Jilin 137000, P.R. China, Department of Cardiology, The Third Hospital of Southern Medical University, Guangzhou, Guangdong 510000, P.R. China, Department of Gerontology, The First Hospital of Jilin University, Changchun, Jilin 130021, P.R. China
  • Pages: 4305-4311
    |
    Published online on: April 24, 2017
       https://doi.org/10.3892/mmr.2017.6503
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Abstract

The present study aimed to investigate the distribution of T follicular helper (Tfh)-cell subsets in patients with hepatitis B virus (HBV) and determine the underlying mechanism of HBV regulation of Tfh cells. The frequency of peripheral blood Tfh subsets was analyzed using flow cytometry. The expression level of programmed cell death‑1 (PD‑1) and prostaglandin E2 (PGE2) was quantified using reverse transcription‑quantitative polymerase chain reaction and western blotting. The PGE2 level in culture supernatant was detected using enzyme‑linked immunosorbent assay. A Transwell chamber was used to co‑culture Tfh cells with HepG2 and HepG2.2.1.5. The percentage of inducible T‑cell costimulator (ICOS)+ and total Tfh cells was high at the immune activation (IA) group; however, it was reduced in the immune tolerance (IT), responders with HBsAg seroconversion (RP) and healthy control (HC) groups. The percentage of PD‑1+ Tfh cells was significantly higher in IA and IT compared with RP and HC. The ratio of PD‑1+/total Tfh cells was positively correlated with the load of HBV DNA; therefore, this ratio may act as an indicator for HBV replication. The expression level of PD‑1 in Tfh cells was higher in the HepG2.2.1.5 co‑cultured group compared with the HepG2 group, this may be due to the high PGE2 expression level in HBV‑infected HepG2.2.1.5 cells. The findings of the present study revealed an imbalanced distribution of PD‑1+ Tfh cells in patients with HBV at different immune phases. Additionally, HBV may upregulate the expression of PD‑1 in Tfh cells by promoting HepG2.2.1.5 to secret PGE2. Identifying the effect of HBV on Tfh‑cell subsets is crucial for improving immuno-based therapy for HBV.
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Copy and paste a formatted citation
Spandidos Publications style
Sui Z, Shi Y, Gao Z, Yang D and Wang Z: Expression of programmed cell death1 in T follicular helper cells is regulated by prostaglandin E2 secreted by HBV-infected HepG2.2.1.5 cells. Mol Med Rep 15: 4305-4311, 2017.
APA
Sui, Z., Shi, Y., Gao, Z., Yang, D., & Wang, Z. (2017). Expression of programmed cell death1 in T follicular helper cells is regulated by prostaglandin E2 secreted by HBV-infected HepG2.2.1.5 cells. Molecular Medicine Reports, 15, 4305-4311. https://doi.org/10.3892/mmr.2017.6503
MLA
Sui, Z., Shi, Y., Gao, Z., Yang, D., Wang, Z."Expression of programmed cell death1 in T follicular helper cells is regulated by prostaglandin E2 secreted by HBV-infected HepG2.2.1.5 cells". Molecular Medicine Reports 15.6 (2017): 4305-4311.
Chicago
Sui, Z., Shi, Y., Gao, Z., Yang, D., Wang, Z."Expression of programmed cell death1 in T follicular helper cells is regulated by prostaglandin E2 secreted by HBV-infected HepG2.2.1.5 cells". Molecular Medicine Reports 15, no. 6 (2017): 4305-4311. https://doi.org/10.3892/mmr.2017.6503
Copy and paste a formatted citation
x
Spandidos Publications style
Sui Z, Shi Y, Gao Z, Yang D and Wang Z: Expression of programmed cell death1 in T follicular helper cells is regulated by prostaglandin E2 secreted by HBV-infected HepG2.2.1.5 cells. Mol Med Rep 15: 4305-4311, 2017.
APA
Sui, Z., Shi, Y., Gao, Z., Yang, D., & Wang, Z. (2017). Expression of programmed cell death1 in T follicular helper cells is regulated by prostaglandin E2 secreted by HBV-infected HepG2.2.1.5 cells. Molecular Medicine Reports, 15, 4305-4311. https://doi.org/10.3892/mmr.2017.6503
MLA
Sui, Z., Shi, Y., Gao, Z., Yang, D., Wang, Z."Expression of programmed cell death1 in T follicular helper cells is regulated by prostaglandin E2 secreted by HBV-infected HepG2.2.1.5 cells". Molecular Medicine Reports 15.6 (2017): 4305-4311.
Chicago
Sui, Z., Shi, Y., Gao, Z., Yang, D., Wang, Z."Expression of programmed cell death1 in T follicular helper cells is regulated by prostaglandin E2 secreted by HBV-infected HepG2.2.1.5 cells". Molecular Medicine Reports 15, no. 6 (2017): 4305-4311. https://doi.org/10.3892/mmr.2017.6503
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