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Detection of fetal epigenetic biomarkers through genome-wide DNA methylation study for non-invasive prenatal diagnosis

  • Authors:
    • Hong‑Dan Wang
    • Lin Liu
    • Hui‑Ru Zhao
    • Qiao‑Fang Hou
    • Jing‑Bin Yan
    • Wei‑Li Shi
    • Qian‑Nan Guo
    • Li Wang
    • Shi‑Xiu Liao
    • Bo‑Feng Zhu
  • View Affiliations / Copyright

    Affiliations: Medical Genetic Institute of Henan Province, Henan Provincial People's Hospital, Zhengzhou University People's Hospital, Zhengzhou, Henan 450003, P.R. China, Department of Cardiovascular Ultrasound, Henan Provincial People's Hospital, Zhengzhou University People's Hospital, Zhengzhou, Henan 450003, P.R. China, Shanghai Children's Hospital, Shanghai Institute of Medical Genetics, Key Laboratory of Embryo Molecular Biology, Ministry of Health of China and Shanghai Key Laboratory of Embryo and Reproduction Engineering, Shanghai 200040, P.R. China, Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an, Shaanxi 710004, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3989-3998
    |
    Published online on: April 25, 2017
       https://doi.org/10.3892/mmr.2017.6506
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Abstract

The discovery of cell-free DNA fetal (cff DNA) in maternal plasma during pregnancy provides a novel perspective for the development of non‑invasive prenatal diagnosis (NIPD). Against the background of maternal DNA, the use of the relatively low concentration of cff DNA is limited in NIPD. Therefore, in order to overcome the complication of the background of maternal DNA and expand the scope of cff DNA application in clinical practice, it is necessary to identify novel universal fetal‑specific DNA markers. The GeneChip Human Promoter 1.0R Array set was used in the present study to analyze the methylation status of 12 placental tissue and maternal peripheral blood whole‑genome DNA samples. In total, 5 fetus differential hypermethylation regions and 6 fetus differential hypomethylation regions were identified. In order to verify the 11 selected methylation regions and detect the differential CpG sites in these regions, a bisulfate direct sequencing strategy was used. In total, 87 fetal differential methylation CpG sites were identified from 123 CpG sites. The detection of fetal differential methylation DNA regions and CpG sites may be instrumental in the development of efficient NIPD and in the expansion of its application in other disorders.
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Copy and paste a formatted citation
Spandidos Publications style
Wang HD, Liu L, Zhao HR, Hou QF, Yan JB, Shi WL, Guo QN, Wang L, Liao SX, Zhu BF, Zhu BF, et al: Detection of fetal epigenetic biomarkers through genome-wide DNA methylation study for non-invasive prenatal diagnosis. Mol Med Rep 15: 3989-3998, 2017.
APA
Wang, H., Liu, L., Zhao, H., Hou, Q., Yan, J., Shi, W. ... Zhu, B. (2017). Detection of fetal epigenetic biomarkers through genome-wide DNA methylation study for non-invasive prenatal diagnosis. Molecular Medicine Reports, 15, 3989-3998. https://doi.org/10.3892/mmr.2017.6506
MLA
Wang, H., Liu, L., Zhao, H., Hou, Q., Yan, J., Shi, W., Guo, Q., Wang, L., Liao, S., Zhu, B."Detection of fetal epigenetic biomarkers through genome-wide DNA methylation study for non-invasive prenatal diagnosis". Molecular Medicine Reports 15.6 (2017): 3989-3998.
Chicago
Wang, H., Liu, L., Zhao, H., Hou, Q., Yan, J., Shi, W., Guo, Q., Wang, L., Liao, S., Zhu, B."Detection of fetal epigenetic biomarkers through genome-wide DNA methylation study for non-invasive prenatal diagnosis". Molecular Medicine Reports 15, no. 6 (2017): 3989-3998. https://doi.org/10.3892/mmr.2017.6506
Copy and paste a formatted citation
x
Spandidos Publications style
Wang HD, Liu L, Zhao HR, Hou QF, Yan JB, Shi WL, Guo QN, Wang L, Liao SX, Zhu BF, Zhu BF, et al: Detection of fetal epigenetic biomarkers through genome-wide DNA methylation study for non-invasive prenatal diagnosis. Mol Med Rep 15: 3989-3998, 2017.
APA
Wang, H., Liu, L., Zhao, H., Hou, Q., Yan, J., Shi, W. ... Zhu, B. (2017). Detection of fetal epigenetic biomarkers through genome-wide DNA methylation study for non-invasive prenatal diagnosis. Molecular Medicine Reports, 15, 3989-3998. https://doi.org/10.3892/mmr.2017.6506
MLA
Wang, H., Liu, L., Zhao, H., Hou, Q., Yan, J., Shi, W., Guo, Q., Wang, L., Liao, S., Zhu, B."Detection of fetal epigenetic biomarkers through genome-wide DNA methylation study for non-invasive prenatal diagnosis". Molecular Medicine Reports 15.6 (2017): 3989-3998.
Chicago
Wang, H., Liu, L., Zhao, H., Hou, Q., Yan, J., Shi, W., Guo, Q., Wang, L., Liao, S., Zhu, B."Detection of fetal epigenetic biomarkers through genome-wide DNA methylation study for non-invasive prenatal diagnosis". Molecular Medicine Reports 15, no. 6 (2017): 3989-3998. https://doi.org/10.3892/mmr.2017.6506
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