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Article

Age-associated alterations in constitutively expressed cyclooxygenase-2 immunoreactivity and protein levels in the hippocampus

  • Authors:
    • Hyo Young Jung
    • Dae Young Yoo
    • Jong Whi Kim
    • Hyun Jung Kwon
    • Kwon Young Lee
    • Jung Hoon Choi
    • Dae Won Kim
    • Jin Young Chung
    • Yeo Sung Yoon
    • In Koo Hwang
  • View Affiliations / Copyright

    Affiliations: Department of Anatomy and Cell Biology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University, Seoul 08826, Republic of Korea, Department of Biochemistry and Molecular Biology, Research Institute of Oral Sciences, College of Dentistry, Gangneung‑Wonju National University, Gangneung, Gangwon 25457, Republic of Korea, Department of Anatomy, College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea, Department of Veterinary Internal Medicine and Geriatrics, College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon, Gangwon 24341, Republic of Korea
  • Pages: 4333-4337
    |
    Published online on: April 26, 2017
       https://doi.org/10.3892/mmr.2017.6512
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Abstract

Cyclooxygenase-2 (COX-2) is a known inducible inflammatory mediator. COX-2 is constitutively expressed in the hippocampus and may regulate synaptic plasticity. The present study investigated the age‑associated alterations in white blood cell counts and hippocampal COX‑2 expression in healthy mice using immunohistochemical and western blot analyses at 1 month postnatal (PM1), PM3, PM6, PM12 and PM24. White blood cell counts were significantly decreased in the PM24 group when compared with the PM1 group. In addition, lymphocyte counts were decreased in the PM24 group when compared with all other groups. By contrast, monocyte, neutrophil and eosinophil counts were increased in the PM24 group; however, this did not reach statistical significance. COX‑2 expression was identified in the granule cells of the dentate gyrus and in the pyramidal cells of the hippocampal CA2/3 region. COX‑2 immunoreactivity was maintained until PM18, however, the levels significantly decreased by PM24. These results suggest that, despite alterations in the differential white blood cell counts, the significant decrease in constitutive COX‑2 expression in the hippocampus may be associated with degenerative age-associated alterations in synaptic plasticity in the hippocampus.
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Copy and paste a formatted citation
Spandidos Publications style
Jung HY, Yoo DY, Kim JW, Kwon HJ, Lee KY, Choi JH, Kim DW, Chung JY, Yoon YS, Hwang IK, Hwang IK, et al: Age-associated alterations in constitutively expressed cyclooxygenase-2 immunoreactivity and protein levels in the hippocampus. Mol Med Rep 15: 4333-4337, 2017.
APA
Jung, H.Y., Yoo, D.Y., Kim, J.W., Kwon, H.J., Lee, K.Y., Choi, J.H. ... Hwang, I.K. (2017). Age-associated alterations in constitutively expressed cyclooxygenase-2 immunoreactivity and protein levels in the hippocampus. Molecular Medicine Reports, 15, 4333-4337. https://doi.org/10.3892/mmr.2017.6512
MLA
Jung, H. Y., Yoo, D. Y., Kim, J. W., Kwon, H. J., Lee, K. Y., Choi, J. H., Kim, D. W., Chung, J. Y., Yoon, Y. S., Hwang, I. K."Age-associated alterations in constitutively expressed cyclooxygenase-2 immunoreactivity and protein levels in the hippocampus". Molecular Medicine Reports 15.6 (2017): 4333-4337.
Chicago
Jung, H. Y., Yoo, D. Y., Kim, J. W., Kwon, H. J., Lee, K. Y., Choi, J. H., Kim, D. W., Chung, J. Y., Yoon, Y. S., Hwang, I. K."Age-associated alterations in constitutively expressed cyclooxygenase-2 immunoreactivity and protein levels in the hippocampus". Molecular Medicine Reports 15, no. 6 (2017): 4333-4337. https://doi.org/10.3892/mmr.2017.6512
Copy and paste a formatted citation
x
Spandidos Publications style
Jung HY, Yoo DY, Kim JW, Kwon HJ, Lee KY, Choi JH, Kim DW, Chung JY, Yoon YS, Hwang IK, Hwang IK, et al: Age-associated alterations in constitutively expressed cyclooxygenase-2 immunoreactivity and protein levels in the hippocampus. Mol Med Rep 15: 4333-4337, 2017.
APA
Jung, H.Y., Yoo, D.Y., Kim, J.W., Kwon, H.J., Lee, K.Y., Choi, J.H. ... Hwang, I.K. (2017). Age-associated alterations in constitutively expressed cyclooxygenase-2 immunoreactivity and protein levels in the hippocampus. Molecular Medicine Reports, 15, 4333-4337. https://doi.org/10.3892/mmr.2017.6512
MLA
Jung, H. Y., Yoo, D. Y., Kim, J. W., Kwon, H. J., Lee, K. Y., Choi, J. H., Kim, D. W., Chung, J. Y., Yoon, Y. S., Hwang, I. K."Age-associated alterations in constitutively expressed cyclooxygenase-2 immunoreactivity and protein levels in the hippocampus". Molecular Medicine Reports 15.6 (2017): 4333-4337.
Chicago
Jung, H. Y., Yoo, D. Y., Kim, J. W., Kwon, H. J., Lee, K. Y., Choi, J. H., Kim, D. W., Chung, J. Y., Yoon, Y. S., Hwang, I. K."Age-associated alterations in constitutively expressed cyclooxygenase-2 immunoreactivity and protein levels in the hippocampus". Molecular Medicine Reports 15, no. 6 (2017): 4333-4337. https://doi.org/10.3892/mmr.2017.6512
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