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Acinetobacter baumannii quorum-sensing signalling molecule induces the expression of drug-resistance genes

  • Authors:
    • Yi Dou
    • Fei Song
    • Feng Guo
    • Zengding Zhou
    • Cailian Zhu
    • Jun Xiang
    • Jingning Huan
  • View Affiliations / Copyright

    Affiliations: Department of Burns and Plastic Surgery, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200025, P.R. China, The Ninth People's Hospital, School of Stomatology, Shanghai Jiaotong University, Shanghai Research Institute of Stomatology, Shanghai 200011, P.R. China
    Copyright: © Dou et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4061-4068
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    Published online on: April 28, 2017
       https://doi.org/10.3892/mmr.2017.6528
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Abstract

Quorum-sensing signalling molecules such as N‑acyl homoserine lactones (AHLs) enable certain Gram‑negative bacteria to respond to environmental changes through behaviours, such as biofilm formation and flagellar movement. The present study aimed to identify Acinetobacter baumannii AHLs and assess their influence on antibiotic resistance. A clinical isolate of A. baumannii strain S (AbS) was collected from the wound of a burn patient and high‑performance liquid chromatography and tandem quadrupole or quadrupole time‑of‑flight high‑resolution mass spectrometry was used to identify AbS AHLs. Antibiotic sensitivity was assessed in an AHL‑deficient AbS mutant (AbS‑M), and the expression of drug-resistance genes in the presence of meropenem in AbS, AbS‑M and AbS‑M treated with the AHL N-3-hydroxy-dodecanoyl-homoserine lactone (N‑3‑OH‑C12‑HSL). AbS‑M was more sensitive to meropenem and piperacillin than wild‑type AbS, but resistance was restored by supplementation with N‑3‑OH‑C12‑HSL. In addition, meropenem‑treated AbS‑M expressed lower levels of the drug‑resistance genes oxacillinase 51, AmpC, AdeA and AdeB; treatment with N‑3‑OH‑C12‑HSL also restored the expression of these genes. Overall, the results of the present study indicate that N‑3‑OH‑C12‑HSL may be involved in regulating the expression of drug‑resistance genes in A. baumannii. Therefore, this quorum‑sensing signalling molecule may be an important target for treating multidrug‑resistant A. baumannii infections.
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Copy and paste a formatted citation
Spandidos Publications style
Dou Y, Song F, Guo F, Zhou Z, Zhu C, Xiang J and Huan J: Acinetobacter baumannii quorum-sensing signalling molecule induces the expression of drug-resistance genes. Mol Med Rep 15: 4061-4068, 2017.
APA
Dou, Y., Song, F., Guo, F., Zhou, Z., Zhu, C., Xiang, J., & Huan, J. (2017). Acinetobacter baumannii quorum-sensing signalling molecule induces the expression of drug-resistance genes. Molecular Medicine Reports, 15, 4061-4068. https://doi.org/10.3892/mmr.2017.6528
MLA
Dou, Y., Song, F., Guo, F., Zhou, Z., Zhu, C., Xiang, J., Huan, J."Acinetobacter baumannii quorum-sensing signalling molecule induces the expression of drug-resistance genes". Molecular Medicine Reports 15.6 (2017): 4061-4068.
Chicago
Dou, Y., Song, F., Guo, F., Zhou, Z., Zhu, C., Xiang, J., Huan, J."Acinetobacter baumannii quorum-sensing signalling molecule induces the expression of drug-resistance genes". Molecular Medicine Reports 15, no. 6 (2017): 4061-4068. https://doi.org/10.3892/mmr.2017.6528
Copy and paste a formatted citation
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Spandidos Publications style
Dou Y, Song F, Guo F, Zhou Z, Zhu C, Xiang J and Huan J: Acinetobacter baumannii quorum-sensing signalling molecule induces the expression of drug-resistance genes. Mol Med Rep 15: 4061-4068, 2017.
APA
Dou, Y., Song, F., Guo, F., Zhou, Z., Zhu, C., Xiang, J., & Huan, J. (2017). Acinetobacter baumannii quorum-sensing signalling molecule induces the expression of drug-resistance genes. Molecular Medicine Reports, 15, 4061-4068. https://doi.org/10.3892/mmr.2017.6528
MLA
Dou, Y., Song, F., Guo, F., Zhou, Z., Zhu, C., Xiang, J., Huan, J."Acinetobacter baumannii quorum-sensing signalling molecule induces the expression of drug-resistance genes". Molecular Medicine Reports 15.6 (2017): 4061-4068.
Chicago
Dou, Y., Song, F., Guo, F., Zhou, Z., Zhu, C., Xiang, J., Huan, J."Acinetobacter baumannii quorum-sensing signalling molecule induces the expression of drug-resistance genes". Molecular Medicine Reports 15, no. 6 (2017): 4061-4068. https://doi.org/10.3892/mmr.2017.6528
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