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Article Open Access

MALAT1 participates in ultraviolet B-induced photo-aging via regulation of the ERK/MAPK signaling pathway

  • Authors:
    • Li Lei
    • Qinghai Zeng
    • Jianyun Lu
    • Shu Ding
    • Fang Xia
    • Jian Kang
    • Lina Tan
    • Lihua Gao
    • Liyang Kang
    • Ke Cao
    • Jianda Zhou
    • Rong Xiao
    • Jing Chen
    • Jinhua Huang
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, Third Xiangya Hospital of Central South University, Changsha, Hunan 410013, P.R. China, Department of Oncology, Third Xiangya Hospital of Central South University, Changsha, Hunan 410013, P.R. China, Department of Plastic and Reconstructive Surgery, Third Xiangya Hospital of Central South University, Changsha, Hunan 410013, P.R. China, Department of Dermatology, Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, P.R. China
    Copyright: © Lei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 3977-3982
    |
    Published online on: April 28, 2017
       https://doi.org/10.3892/mmr.2017.6532
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Abstract

Long non-coding RNA (lncRNA), transcripts of >200 bp in length that do not appear to exhibit any coding capacity, are important in the occurrence and development of cancer, cardiovascular and neurological diseases. However, effects of lncRNAs on photo‑aging remain to be elucidated. To explore the potential effects of the lncRNA metastasis‑associated lung adenocarcinoma transcript 1 (MALAT1) on photo‑aging in fibroblasts, MALAT1 expression was silenced in fibroblasts using small interference RNA. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR) was used to examine MALAT1 expression in normal and silenced fibroblasts following irradiation with 60 mJ/cm2 ultraviolet B (UVB) and an ELISA assay was used to identify matrix metalloproteinase‑1 (MMP‑1) content in the cellular supernatant. A β-galactosidase kit was applied to measure the number of senescent cells and a western blot assay was used to detect extracellular signal‑regulated kinase (ERK), c‑Jun N‑terminal kinase (JNK) and p38 phosphorylation levels. RT‑qPCR was additionally used to detect changes in MALAT1 expression following suppression of UVB‑induced reactive oxygen species (ROS) generation with N‑acetyl‑L‑cysteine (NAC). Fibroblasts irradiated with 60 mJ/cm2 UVB demonstrated increased MALAT1 expression, MMP‑1 secretory volume and number of senescent cells, and greater levels of ERK, p38 and JNK phosphorylation. Following silencing of MALAT1 expression in photo‑aged fibroblasts, decreases were observed in MMP‑1 secretory volume, number of senescent cells and phosphorylation levels of ERK. NAC reduced ROS content, however, it did not affect MALAT1 expression. Therefore, it was concluded that MALAT1 may participate in UVB‑induced photo‑aging via regulation of the ERK/mitogen‑activated protein kinase signaling pathway and UVB‑induced MALAT1 expression is independent of ROS generation.
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1 

Ham SA, Kang ES, Lee H, Hwang JS, Yoo T, Paek KS, Park C, Kim JH, Lim DS and Seo HG: PPARδ inhibits UVB-induced secretion of MMP-1 through MKP-7-mediated suppression of JNK signaling. J Invest Dermatol. 133:2593–2600. 2013. View Article : Google Scholar

2 

Stoebner PE and Meunier L: Photoaging of face. Ann Dermatol Venereol. 135:1S21–1S26. 2008.(In French).

3 

Park G, Baek S, Kim JE, Lim TG, Lee CC, Yang H, Kang YG, Park JS, Augustin M, Mrosek M, et al: Flt3 is a target of coumestrol in protecting against UVB-induced skin photoaging. Biochem Pharmacol. 98:473–483. 2015. View Article : Google Scholar

4 

Kim MJ, Woo SW, Kim MS, Park JE and Hwang JK: Anti-photoaging effect of aaptamine in UVB-irradiated human dermal fibroblasts and epidermal keratinocytes. J Asian Nat Prod Res. 16:1139–1147. 2014. View Article : Google Scholar

5 

He X, Bao W, Li X, Chen Z, Che Q, Wang H and Wan XP: The long non-coding RNA HOTAIR is upregulated in endometrial carcinoma and correlates with poor prognosis. Int J Mol Med. 33:325–332. 2014.

6 

Li Y and Wang X: Role of long noncoding RNAs in malignant disease (Review). Mol Med Rep. 13:1463–1469. 2016.

7 

Xu S, Sui S, Zhang J, Bai N, Shi Q, Zhang G, Gao S, You Z, Zhan C, Liu F and Pang D: Downregulation of long noncoding RNA MALAT1 induces epithelial-to-mesenchymal transition via the PI3K-AKT pathway in breast cancer. Int J Clin Exp Pathol. 8:4881–4891. 2015.

8 

Dey BK, Mueller AC and Dutta A: Long non-coding RNAs as emerging regulators of differentiation, development, and disease. Transcription. 5:e9440142014. View Article : Google Scholar :

9 

Uchida S and Dimmeler S: Long noncoding RNAs in cardiovascular diseases. Circ Res. 116:737–750. 2015. View Article : Google Scholar

10 

Liu JY, Yao J, Li XM, Song YC, Wang XQ, Li YJ, Yan B and Jiang Q: Pathogenic role of lncRNA-MALAT1 in endothelial cell dysfunction in diabetes mellitus. Cell Death Dis. 5:e15062014. View Article : Google Scholar :

11 

Wei Y and Niu B: Role of MALAT1 as a prognostic factor for survival in various cancers: A systematic review of the literature with meta-analysis. Dis Markers. 2015:1646352015. View Article : Google Scholar :

12 

Wu XS, Wang XA, Wu WG, Hu YP, Li ML, Ding Q, Weng H, Shu YJ, Liu TY, Jiang L, et al: MALAT1 promotes the proliferation and metastasis of gallbladder cancer cells by activating the ERK/MAPK pathway. Cancer Biol Ther. 15:806–814. 2014. View Article : Google Scholar :

13 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar

14 

Zeng Q, Wang Q, Chen X, Xia K, Tang J, Zhou X, Cheng Y, Chen Y, Huang L, Xiang H, et al: Analysis of lncRNAs expression in UVB-induced stress responses of melanocytes. J Dermatol Sci. 81:53–60. 2016. View Article : Google Scholar

15 

Syed DN, Khan MI, Shabbir M and Mukhtar H: MicroRNAs in skin response to UV radiation. Curr Drug Targets. 14:1128–1134. 2013. View Article : Google Scholar :

16 

Li W, Zhou BR, Hua LJ, Guo Z and Luo D: Differential miRNA profile on photoaged primary human fibroblasts irradiated with ultraviolet A. Tumour Biol. 34:3491–3500. 2013. View Article : Google Scholar

17 

Song J, Liu P, Yang Z, Li L, Su H, Lu N and Peng Z: MiR-155 negatively regulates c-Jun expression at the post-transcriptional level in human dermal fibroblasts in vitro: Implications in UVA irradiation-induced photoaging. Cell Physiol Biochem. 29:331–340. 2012. View Article : Google Scholar

18 

Hall JR, Messenger ZJ, Tam HW, Phillips SL, Recio L and Smart RC: Long noncoding RNA lincRNA-p21 is the major mediator of UVB-induced and p53-dependent apoptosis in keratinocytes. Cell Death Dis. 6:e17002015. View Article : Google Scholar :

19 

Gutschner T, Hammerle M and Diederichs S: MALAT1-a paradigm for long noncoding RNA function in cancer. J Mol Med (Berl). 91:791–801. 2013. View Article : Google Scholar

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Copy and paste a formatted citation
Spandidos Publications style
Lei L, Zeng Q, Lu J, Ding S, Xia F, Kang J, Tan L, Gao L, Kang L, Cao K, Cao K, et al: MALAT1 participates in ultraviolet B-induced photo-aging via regulation of the ERK/MAPK signaling pathway. Mol Med Rep 15: 3977-3982, 2017.
APA
Lei, L., Zeng, Q., Lu, J., Ding, S., Xia, F., Kang, J. ... Huang, J. (2017). MALAT1 participates in ultraviolet B-induced photo-aging via regulation of the ERK/MAPK signaling pathway. Molecular Medicine Reports, 15, 3977-3982. https://doi.org/10.3892/mmr.2017.6532
MLA
Lei, L., Zeng, Q., Lu, J., Ding, S., Xia, F., Kang, J., Tan, L., Gao, L., Kang, L., Cao, K., Zhou, J., Xiao, R., Chen, J., Huang, J."MALAT1 participates in ultraviolet B-induced photo-aging via regulation of the ERK/MAPK signaling pathway". Molecular Medicine Reports 15.6 (2017): 3977-3982.
Chicago
Lei, L., Zeng, Q., Lu, J., Ding, S., Xia, F., Kang, J., Tan, L., Gao, L., Kang, L., Cao, K., Zhou, J., Xiao, R., Chen, J., Huang, J."MALAT1 participates in ultraviolet B-induced photo-aging via regulation of the ERK/MAPK signaling pathway". Molecular Medicine Reports 15, no. 6 (2017): 3977-3982. https://doi.org/10.3892/mmr.2017.6532
Copy and paste a formatted citation
x
Spandidos Publications style
Lei L, Zeng Q, Lu J, Ding S, Xia F, Kang J, Tan L, Gao L, Kang L, Cao K, Cao K, et al: MALAT1 participates in ultraviolet B-induced photo-aging via regulation of the ERK/MAPK signaling pathway. Mol Med Rep 15: 3977-3982, 2017.
APA
Lei, L., Zeng, Q., Lu, J., Ding, S., Xia, F., Kang, J. ... Huang, J. (2017). MALAT1 participates in ultraviolet B-induced photo-aging via regulation of the ERK/MAPK signaling pathway. Molecular Medicine Reports, 15, 3977-3982. https://doi.org/10.3892/mmr.2017.6532
MLA
Lei, L., Zeng, Q., Lu, J., Ding, S., Xia, F., Kang, J., Tan, L., Gao, L., Kang, L., Cao, K., Zhou, J., Xiao, R., Chen, J., Huang, J."MALAT1 participates in ultraviolet B-induced photo-aging via regulation of the ERK/MAPK signaling pathway". Molecular Medicine Reports 15.6 (2017): 3977-3982.
Chicago
Lei, L., Zeng, Q., Lu, J., Ding, S., Xia, F., Kang, J., Tan, L., Gao, L., Kang, L., Cao, K., Zhou, J., Xiao, R., Chen, J., Huang, J."MALAT1 participates in ultraviolet B-induced photo-aging via regulation of the ERK/MAPK signaling pathway". Molecular Medicine Reports 15, no. 6 (2017): 3977-3982. https://doi.org/10.3892/mmr.2017.6532
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