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Article

Annexin A2 functions downstream of c‑Jun N‑terminal kinase to promote skin fibroblast cell migration

  • Authors:
    • Youpei Wang
    • Xinmei Wu
    • Qing Wang
    • Meiqin Zheng
    • Lingxia Pang
  • View Affiliations / Copyright

    Affiliations: Clinical Examination Center, The Affiliated Eye Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China, The Second Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China, Function Experiment Teaching Center of Wenzhou Medical University, Wenzhou, Zhejiang 325035, P.R. China
  • Pages: 4207-4216
    |
    Published online on: May 2, 2017
       https://doi.org/10.3892/mmr.2017.6535
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Abstract

Delayed healing of skin wounds is one of the outcomes of diabetes mellitus (DM), a condition that affects a significant number of patients worldwide. However, the underlying mechanisms remain unknown. In order to examine proteome alterations in DM, a rat model of type 1 diabetes was developed using streptozotocin injections. The proteomic responses of normal and DM rat skin were analyzed by two‑dimensional electrophoresis, and differentially expressed proteins were identified using a liquid chromatography/mass spectrometry system. DM induced 36 and repressed 41 differentially expressed proteins, respectively. Altered proteins were involved in a number of biological processes, including RNA and protein metabolism, the tricarboxylic acid cycle, glycolysis, cytoskeleton regulation, hydrogen detoxification and calcium‑mediated signal transduction. In addition, overexpression of annexin A2, one of the signaling proteins altered by DM, accelerated the rate of human skin fibroblast cell migration. Application of SP600125, an inhibitor of a key regulator of cell migration c‑Jun N‑terminal kinase (JNK), inhibited the migration of normal cells. By contrast, SP600125 treatment did not inhibit the migration of annexin A2‑overexpressed cells, indicating that annexin A2 may function downstream of JNK. In conclusion, the results of the present study reveal the potential proteomic responses to DM in skin tissues, and demonstrate a positive functional role of annexin A2 in fibroblast cell migration.
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Copy and paste a formatted citation
Spandidos Publications style
Wang Y, Wu X, Wang Q, Zheng M and Pang L: Annexin A2 functions downstream of c‑Jun N‑terminal kinase to promote skin fibroblast cell migration. Mol Med Rep 15: 4207-4216, 2017.
APA
Wang, Y., Wu, X., Wang, Q., Zheng, M., & Pang, L. (2017). Annexin A2 functions downstream of c‑Jun N‑terminal kinase to promote skin fibroblast cell migration. Molecular Medicine Reports, 15, 4207-4216. https://doi.org/10.3892/mmr.2017.6535
MLA
Wang, Y., Wu, X., Wang, Q., Zheng, M., Pang, L."Annexin A2 functions downstream of c‑Jun N‑terminal kinase to promote skin fibroblast cell migration". Molecular Medicine Reports 15.6 (2017): 4207-4216.
Chicago
Wang, Y., Wu, X., Wang, Q., Zheng, M., Pang, L."Annexin A2 functions downstream of c‑Jun N‑terminal kinase to promote skin fibroblast cell migration". Molecular Medicine Reports 15, no. 6 (2017): 4207-4216. https://doi.org/10.3892/mmr.2017.6535
Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Wu X, Wang Q, Zheng M and Pang L: Annexin A2 functions downstream of c‑Jun N‑terminal kinase to promote skin fibroblast cell migration. Mol Med Rep 15: 4207-4216, 2017.
APA
Wang, Y., Wu, X., Wang, Q., Zheng, M., & Pang, L. (2017). Annexin A2 functions downstream of c‑Jun N‑terminal kinase to promote skin fibroblast cell migration. Molecular Medicine Reports, 15, 4207-4216. https://doi.org/10.3892/mmr.2017.6535
MLA
Wang, Y., Wu, X., Wang, Q., Zheng, M., Pang, L."Annexin A2 functions downstream of c‑Jun N‑terminal kinase to promote skin fibroblast cell migration". Molecular Medicine Reports 15.6 (2017): 4207-4216.
Chicago
Wang, Y., Wu, X., Wang, Q., Zheng, M., Pang, L."Annexin A2 functions downstream of c‑Jun N‑terminal kinase to promote skin fibroblast cell migration". Molecular Medicine Reports 15, no. 6 (2017): 4207-4216. https://doi.org/10.3892/mmr.2017.6535
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