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Induction of cell cycle arrest by increasing GTP‑RhoA levels via Taxol‑induced microtubule polymerization in renal cell carcinoma

  • Authors:
    • Yu Ren
    • Xue Wang
    • Zhongguan Lou
    • Shuaishuai Huang
    • Haihui Zhuang
    • Yuduo Wang
    • Guobin Weng
    • Ping Wang
  • View Affiliations / Copyright

    Affiliations: Department of Urologic Surgery, Ningbo Urology and Nephrology Hospital, Ningbo, Zhejiang 315000, P.R. China, Center for Translational Medicine, Ningbo University School of Medicine, Ningbo, Zhejiang 315211, P.R. China
    Copyright: © Ren et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 4273-4279
    |
    Published online on: May 3, 2017
       https://doi.org/10.3892/mmr.2017.6543
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Abstract

Renal cell carcinoma (RCC) is the most common neoplasm of the kidney in adults, accounting for ~3% of adult malignancies. Understanding the underlying mechanism of RCC tumorigenesis is necessary to improve patient survival. The present study revealed that Taxol‑induced microtubule (MT) polymerization causes cell cycle arrest and an increase in guanosine triphosphate‑Ras homology gene family, member A (GTP‑RhoA) protein expression. Disruption of Taxol‑induced MT polymerization reversed GTP‑RhoA expression and cell cycle arrest. The localization and redistribution of MTs and RhoA were consistent in cells with MT bundles and those without. Decreased GTP‑RhoA had no marked effect on Taxol‑induced MT bundling, however, it reduced the proportion of cells in G2/M phase. Taken together, Taxol‑induced MT polymerization regulated the protein expression levels of GTP‑RhoA and cell cycle arrest. However, the alteration in GTP‑RhoA expression did not influence MT arrangement, suggesting that GTP‑RhoA serves a pivotal role in Taxol‑induced MT polymerization and cell cycle arrest in RCC.
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Copy and paste a formatted citation
Spandidos Publications style
Ren Y, Wang X, Lou Z, Huang S, Zhuang H, Wang Y, Weng G and Wang P: Induction of cell cycle arrest by increasing GTP‑RhoA levels via Taxol‑induced microtubule polymerization in renal cell carcinoma. Mol Med Rep 15: 4273-4279, 2017.
APA
Ren, Y., Wang, X., Lou, Z., Huang, S., Zhuang, H., Wang, Y. ... Wang, P. (2017). Induction of cell cycle arrest by increasing GTP‑RhoA levels via Taxol‑induced microtubule polymerization in renal cell carcinoma. Molecular Medicine Reports, 15, 4273-4279. https://doi.org/10.3892/mmr.2017.6543
MLA
Ren, Y., Wang, X., Lou, Z., Huang, S., Zhuang, H., Wang, Y., Weng, G., Wang, P."Induction of cell cycle arrest by increasing GTP‑RhoA levels via Taxol‑induced microtubule polymerization in renal cell carcinoma". Molecular Medicine Reports 15.6 (2017): 4273-4279.
Chicago
Ren, Y., Wang, X., Lou, Z., Huang, S., Zhuang, H., Wang, Y., Weng, G., Wang, P."Induction of cell cycle arrest by increasing GTP‑RhoA levels via Taxol‑induced microtubule polymerization in renal cell carcinoma". Molecular Medicine Reports 15, no. 6 (2017): 4273-4279. https://doi.org/10.3892/mmr.2017.6543
Copy and paste a formatted citation
x
Spandidos Publications style
Ren Y, Wang X, Lou Z, Huang S, Zhuang H, Wang Y, Weng G and Wang P: Induction of cell cycle arrest by increasing GTP‑RhoA levels via Taxol‑induced microtubule polymerization in renal cell carcinoma. Mol Med Rep 15: 4273-4279, 2017.
APA
Ren, Y., Wang, X., Lou, Z., Huang, S., Zhuang, H., Wang, Y. ... Wang, P. (2017). Induction of cell cycle arrest by increasing GTP‑RhoA levels via Taxol‑induced microtubule polymerization in renal cell carcinoma. Molecular Medicine Reports, 15, 4273-4279. https://doi.org/10.3892/mmr.2017.6543
MLA
Ren, Y., Wang, X., Lou, Z., Huang, S., Zhuang, H., Wang, Y., Weng, G., Wang, P."Induction of cell cycle arrest by increasing GTP‑RhoA levels via Taxol‑induced microtubule polymerization in renal cell carcinoma". Molecular Medicine Reports 15.6 (2017): 4273-4279.
Chicago
Ren, Y., Wang, X., Lou, Z., Huang, S., Zhuang, H., Wang, Y., Weng, G., Wang, P."Induction of cell cycle arrest by increasing GTP‑RhoA levels via Taxol‑induced microtubule polymerization in renal cell carcinoma". Molecular Medicine Reports 15, no. 6 (2017): 4273-4279. https://doi.org/10.3892/mmr.2017.6543
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