Effects of TAZ on human dental pulp stem cell proliferation and migration

Tian,Songbo; Tian,Xiaochao; Liu,Yanping; Dong,Fusheng; Wang,Jie; Liu,Xuqian; Zhang,Zhiyong; Chen,Huizhen

doi:10.3892/mmr.2017.6550

Effects of TAZ on human dental pulp stem cell proliferation and migration

Authors:
- Songbo Tian
- Xiaochao Tian
- Yanping Liu
- Fusheng Dong
- Jie Wang
- Xuqian Liu
- Zhiyong Zhang
- Huizhen Chen
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Affiliations: Department of Oral Medicine, Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China, Department of Cardiology, Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China, Physical Examination Center, Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China, Department of Oral and Maxillofacial Surgery, College of Stomatology, Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China, Department of Oral Pathology, College of Stomatology, Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China
Published online on: May 3, 2017 https://doi.org/10.3892/mmr.2017.6550
Pages: 4326-4332

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Abstract

Transcriptional coactivator with PDZ‑binding motif (TAZ) acts as the key downstream regulatory target in the Hippo signaling pathway. TAZ overexpression has been reported to promote cellular proliferation and induce epithelial‑mesenchymal transition in human mammary epithelial cells. However, the effects of TAZ in the regulation of human dental pulp stem cell (hDPSC) proliferation and migration, as well as the molecular mechanisms underlying its actions, remain to be elucidated. The present study demonstrated that TAZ was expressed in hDPSCs. TAZ silencing, following hDPSC transfection with TAZ‑specific small interfering (si)RNA (siTAZ), inhibited cellular proliferation and migration in vitro. These effects appeared to be associated with the downregulation of connecting tissue growth factor (CTGF) and cysteine‑rich angiogenic inducer (Cyr) 61 expression. Further investigation of the mechanisms underlying the actions of TAZ in hDPSCs revealed that TAZ silencing suppressed CTGF and Cyr61 expression by interfering with transforming growth factor (TGF)‑β signaling pathways. The present results suggested that TAZ may be implicated in the proliferation and migration of hDPSCs, through the modulation of CTGF and Cyr61 expression via a TGF‑β‑dependent signaling pathway.

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