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Article

Punicalagin suppresses the proliferation and invasion of cervical cancer cells through inhibition of the β-catenin pathway

  • Authors:
    • Jianming Tang
    • Bingshu Li
    • Shasha Hong
    • Cheng Liu
    • Jie Min
    • Ming Hu
    • Yang Li
    • Yaodan Liu
    • Li Hong
  • View Affiliations / Copyright

    Affiliations: Department of Gynaecology and Obstetrics, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China
  • Pages: 1439-1444
    |
    Published online on: June 6, 2017
       https://doi.org/10.3892/mmr.2017.6687
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Abstract

Natural botanical drugs have attracted attention due to their cancer chemopreventive and chemotherapeutic properties in cancer. Punicalagin (PUN) is the major bioactive component of pomegranate peel, and has been shown to have antioxidant, anti-inflammatory, antiviral, antiproliferation and anticancer properties. PUN has been shown to induce apoptosis in several cancer cell lines. The aim of the present study was to investigate the effect of PUN on HeLa human cervical cancer cells in vitro. The viability of the HeLa cells was assessed following treatment with PUN (0, 12.5, 25, 50, 100 and 200 µM) for 24, 36 and 48 h using a Cell Counting Kit‑8 assay. In addition, the cell cycle distribution, protein expression levels of B‑cell lymphoma 2 (Bcl‑2)‑associated X protein (Bax), Bcl‑2, tissue inhibitor of metalloproteinase (TIMP)-2, TIMP‑3 and the β‑catenin pathway, and the activities of matrix metalloproteinase (MMP)‑2 and MMP‑9 were analyzed following treatment with PUN (0, 25, 50 and 100 µM) for 36 h using cell cycle analysis, western blot analysis and gelatin zymography, respectively. In addition, a wound‑healing assay was used to detect cell migration. PUN led to a number of effects on the HeLa cells, including the inhibition of cell proliferation and cell migration, downregulation of MMP‑2 and MMP‑9, upregulation of TIMP‑2 and TIMP‑3, cell‑cycle arrest in the G1 phase, induction of apoptosis via alterations of Bcl‑2 and Bax, and downregulation of β‑catenin and its downstream proteins, cyclin D1 and c-myc. These results suggested that PUN may have chemopreventive and chemotherapeutic effects against cervical cancer in humans through inhibition of the β-catenin signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Tang J, Li B, Hong S, Liu C, Min J, Hu M, Li Y, Liu Y and Hong L: Punicalagin suppresses the proliferation and invasion of cervical cancer cells through inhibition of the β-catenin pathway. Mol Med Rep 16: 1439-1444, 2017.
APA
Tang, J., Li, B., Hong, S., Liu, C., Min, J., Hu, M. ... Hong, L. (2017). Punicalagin suppresses the proliferation and invasion of cervical cancer cells through inhibition of the β-catenin pathway. Molecular Medicine Reports, 16, 1439-1444. https://doi.org/10.3892/mmr.2017.6687
MLA
Tang, J., Li, B., Hong, S., Liu, C., Min, J., Hu, M., Li, Y., Liu, Y., Hong, L."Punicalagin suppresses the proliferation and invasion of cervical cancer cells through inhibition of the β-catenin pathway". Molecular Medicine Reports 16.2 (2017): 1439-1444.
Chicago
Tang, J., Li, B., Hong, S., Liu, C., Min, J., Hu, M., Li, Y., Liu, Y., Hong, L."Punicalagin suppresses the proliferation and invasion of cervical cancer cells through inhibition of the β-catenin pathway". Molecular Medicine Reports 16, no. 2 (2017): 1439-1444. https://doi.org/10.3892/mmr.2017.6687
Copy and paste a formatted citation
x
Spandidos Publications style
Tang J, Li B, Hong S, Liu C, Min J, Hu M, Li Y, Liu Y and Hong L: Punicalagin suppresses the proliferation and invasion of cervical cancer cells through inhibition of the β-catenin pathway. Mol Med Rep 16: 1439-1444, 2017.
APA
Tang, J., Li, B., Hong, S., Liu, C., Min, J., Hu, M. ... Hong, L. (2017). Punicalagin suppresses the proliferation and invasion of cervical cancer cells through inhibition of the β-catenin pathway. Molecular Medicine Reports, 16, 1439-1444. https://doi.org/10.3892/mmr.2017.6687
MLA
Tang, J., Li, B., Hong, S., Liu, C., Min, J., Hu, M., Li, Y., Liu, Y., Hong, L."Punicalagin suppresses the proliferation and invasion of cervical cancer cells through inhibition of the β-catenin pathway". Molecular Medicine Reports 16.2 (2017): 1439-1444.
Chicago
Tang, J., Li, B., Hong, S., Liu, C., Min, J., Hu, M., Li, Y., Liu, Y., Hong, L."Punicalagin suppresses the proliferation and invasion of cervical cancer cells through inhibition of the β-catenin pathway". Molecular Medicine Reports 16, no. 2 (2017): 1439-1444. https://doi.org/10.3892/mmr.2017.6687
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