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Protective role of 17β-estradiol on tumor necrosis factor-α-induced apoptosis in human nucleus pulposus cells

  • Authors:
    • Huan Liu
    • Si‑Dong Yang
    • Ying Xu
    • Sheng‑Hua Ning
    • Tao Wang
    • Da‑Long Yang
    • Wen‑Yuan Ding
  • View Affiliations / Copyright

    Affiliations: Department of Spine Surgery, The Third Hospital of Hebei Medical University, Shijiazhuang, Hebei 050051, P.R. China, Department of Cardiology, The Traditional Chinese Medicine Hospital of Hebei Medical University, Shijiazhuang, Hebei 050011, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1093-1100
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    Published online on: June 6, 2017
       https://doi.org/10.3892/mmr.2017.6690
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Abstract

The molecular mechanisms underlying protection and pathogenesis in spinal degenerative diseases remain unclear. Tumor necrosis factor-α (TNF-α) has been demonstrated to induce apoptosis of inte rvertebral disc (IVD) cells during IVD degeneration, and 17β‑estradiol (17β‑E2) has a protective effect against IVD cell apoptosis. However, the underlying molecular mechanism by which 17β‑E2 protects nucleus pulposus (NP) cells remains to be investigated. The aim of the present study was to evaluate whether 17β‑E2 modulates apoptosis of human NP cells induced by TNF‑α. In addition, the concentration‑response effect of 17β‑E2 on human NP cells was investigated. Human NP cells were cultured in complete medium, which was replaced every three days until the culture was ~80% confluent. Cells were treated with 100 ng/ml TNF‑α for 48 h, with or without pretreatment with various concentrations of 17β‑E2, and ICI 182,780, for 30 min. Morphologic alterations characteristic of apoptosis were observed by inverted phase‑contrast microscopy and Hoechst 33258 staining; the apoptosis rate was analyzed by flow cytometry. A Cell Counting kit‑8 assay was used to assess cell proliferation. Furthermore, caspase‑3 activity was determined and proteins associated with apoptosis were analyzed by western blotting. The level of apoptosis and caspase‑3 activity in human NP cells increased, whereas proliferation and the expression of poly ADP‑ribose polymerase decreased following TNF‑α treatment. These effects of TNF‑α were abolished by pretreatment with 17β‑E2 in a concentration‑dependent manner. The results of the present study indicated that 17β‑E2 serves a critical role in the survival of degenerative human NP cells.
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View References

1 

Zhao CQ, Jiang LS and Dai LY: Programmed cell death in intervertebral disc degeneration. Apoptosis. 11:2079–2088. 2006. View Article : Google Scholar : PubMed/NCBI

2 

Buckwalter JA: Aging and degeneration human intervertebral disc. Spine (Phila Pa 1976). 20:1307–1314. 1995. View Article : Google Scholar : PubMed/NCBI

3 

Ferguson SJ, Ito K and Nolte LP: Fluid flow and convection transport of solutes within the intervertebral disc. J Biomech. 37:213–221. 2004. View Article : Google Scholar : PubMed/NCBI

4 

Anderson DG, Li X and Balian G: A fibronectin fragment alters the metabolism by rabbit intervertebral disc cells in vitro. Spine (Phila Pa 1976). 30:1242–1246. 2005. View Article : Google Scholar : PubMed/NCBI

5 

Podichetty VK: The aging spine: The role of inflammatory mediators in intervertebral disc degeneration. Cell Mol Biol (Noisy-le-grand). 53:4–18. 2007.PubMed/NCBI

6 

Wang H, Ding W, Yang D, Gu T, Yang S and Bai Z: Different concentrations of 17β-estradiol modulates apoptosis induced by interleukin-1β in rat annulus firosus cells. Mol Med Rep. 10:2745–2751. 2014.PubMed/NCBI

7 

Zhou GQ, Yang F, Leung VVL and Cheung KMC: Molecular and cellular biology of the intervertebral disc and the use of animal models. Current Orthopaedics. 22:267–273. 2008. View Article : Google Scholar

8 

Le Maitre CL, Freemont AJ and Hoyland JA: Accelerated cellular senescence in degenerate intervertebral discs: A possible role in the pathogenesis of intervertebral disc degeneration. Arthritis Res Ther. 9:R452007. View Article : Google Scholar : PubMed/NCBI

9 

Boos N, Weissbach S, Rohrbach H, Weiler C, Spratt KF and Nerlich AG: Classification of age-related changes in lumbar intervertebral discs: 2002 Volvo Award in basic science. Spine (Phila Pa 1976). 27:2631–2644. 2002. View Article : Google Scholar : PubMed/NCBI

10 

Szlosarek P, Charles KA and Balkwill FR: Tumour necrosis factor-alpha as atumour promoter. Eur J Cancer. 42:745–750. 2006. View Article : Google Scholar : PubMed/NCBI

11 

Bharadwaj U, Marin-Muller C, Li M, Chen C and Yao Q: Mesothelin confers pancreatic cancer cell resistance to TNF-a-induced apoptosis through Akt/PI3K/NF-κB activation and IL-6/Mcl-1 overexpression. Mol Cancer. 10:1062011. View Article : Google Scholar : PubMed/NCBI

12 

Le Maitre CL, Freemont AJ and Hoyland JA: The role of interleukin-1 in the pathogenesis of human Intervertebral disc degeneration. Arthritis Res Ther. 7:R732–R745. 2005. View Article : Google Scholar : PubMed/NCBI

13 

Wang J, Markova D, Anderson DG, Zheng Z, Shapiro IM and Risbud MV: TNF-α and IL-1β promote a disintegrin-like and metalloprotease with thrombospondin type I motif-5-mediated aggrecan degradation through syndecan-4 in intervertebral disc. J Biol Chem. 286:39738–39749. 2011. View Article : Google Scholar : PubMed/NCBI

14 

Lee S, Moon CS, Sul D, Lee J, Bae M, Hong Y, Lee M, Choi S, Derby R, Kim BJ, et al: Comparison of growth factor and cytokine expression in patients with degenerated disc disease and herniated nucleus pulposus. Clin Biochem. 42:1504–1511. 2009. View Article : Google Scholar : PubMed/NCBI

15 

Mourão AF, Caetano-Lopes J, Costa P, Canhão H, Santos MJ, Pinto P, Brito I, Nicola P, Cavaleiro J, Teles J, et al: Tumor necrosis factor-alpha-308 genotypes influence inflammatory activity and TNF-alpha serum concentrations in children with juvenile idiopathic arthritis. J Rheumatol. 36:837–842. 2009.PubMed/NCBI

16 

Zhang CC, Cui GP, Hu JG, Xiao YZ, Zhou XS, Shao C, Lin Q and Zhou JS: Effects of adenoviral vector expressing hIGF-1 on apoptosis in nucleus pulposus cells in vitro. Int J Mol Med. 33:401–405. 2014.PubMed/NCBI

17 

de Souza Grava AL, Ferrari LF and Defino HL: Cytokine inhibition and time-related influence of inflammatory stimuli on the hyperalgesia induced by the nucleus pulposus. Eur Spine J. 21:537–545. 2012. View Article : Google Scholar : PubMed/NCBI

18 

Le Maitre CL, Hoyland JA and Freemont AJ: Catabolic cytokine expression in degenerate and herniated human intervertebral discs: IL-1beta and TNFalpha expression profile. Arthritis Res Ther. 9:R772007. View Article : Google Scholar : PubMed/NCBI

19 

Ackermann S, Hiller S, Osswald H, Lösle M, Grenz A and Hambrock A: 17beta-Estradiol modulates apoptosis in pancreatic beta-cells by specific involvement of the sulfonylurea receptor (SUR) isoform SUR1. J Biol Chem. 284:4905–4913. 2009. View Article : Google Scholar : PubMed/NCBI

20 

Yamaguchi A, Nozawa K, Fujishiro M, Kawasaki M, Takamori K, Ogawa H, Sekigawa I and Takasaki Y: Estrogen inhibits apoptosis and promotes CC motif chemokine ligand 13 expression on synovial fibroblasts in rheumatoid arthritis. Immunopharmacol Immunotoxicol. 34:852–857. 2012. View Article : Google Scholar : PubMed/NCBI

21 

Hattori Y, Kojima T, Kato D, Matsubara H, Takigawa M and Ishiguro N: A selective estrogen receptor modulator inhibits tumor necrosis factor-α-induced apoptosis through the ERK1/2 signaling pathway in human chondrocytes. Biochem Biophys Res Commun. 421:418–424. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Yang SD, Ma L, Gu TX, Ding WY, Zhang F, Shen Y, Zhang YZ, Yang DL, Zhang D, Sun YP and Song YL: 17β-Estradiol protects against apoptosis induced by levofloxacin in rat nucleus pulposus cells by upregulating integrin α2β1. Apoptosis. 19:789–800. 2014. View Article : Google Scholar : PubMed/NCBI

23 

Bozzo C, Graziola F, Chiocchetti A and Canonico PL: Estrogen and beta-amyloid toxicity: Role of integrin and PI3-K. Mol Cell Neurosci. 45:85–91. 2010. View Article : Google Scholar : PubMed/NCBI

24 

Nilsen J and Brinton R Diaz: Mechanism of estrogen-mediated neuroprotection: Regulation of mitochondrial calcium and Bcl-2 expression. Proc Natl Acad Sci USA. 100:2842–2847. 2003. View Article : Google Scholar : PubMed/NCBI

25 

Yu X, Zhang X, Dhakal IB, Beggs M, Kadlubar S and Luo D: Induction of cell proliferation and survival genes by estradiol-repressed microRNAs in breast cancer cells. BMC Cancer. 12:292012. View Article : Google Scholar : PubMed/NCBI

26 

Ge C, Yu M and Zhang C: G Protein-Coupled Receptor 30 mediates estrogen-induced proliferation of primordial germ cells via EGFR/Akt/β-catenin signaling pathway. Endocrinology. 153:3504–3516. 2012. View Article : Google Scholar : PubMed/NCBI

27 

Ding F, Shao ZW, Yang SH, Wu Q, Gao F and Xiong LM: Role of mitochondrial pathway in compression-induced apoptosis of nucleus pulposus cells. Apoptosis. 17:579–90. 2012. View Article : Google Scholar : PubMed/NCBI

28 

Nardinocchi L, Puca R, Sacchi A and D'Orazi G: Inhibition of HIF-1alpha activity by homeodomain-interacting protein kinase-2 correlates with sensitization of chemoresistant cells to undergo apoptosis. Mol Cancer. 8:12009. View Article : Google Scholar : PubMed/NCBI

29 

Gruber HE, Yamaguchi D, Ingram J, Leslie K, Huang W, Miller TA and Hanley EN Jr: Expression and localization of estrogen receptor-beta in annulus cells of the human intervertebral disc and the mitogenic effect of 17-beta-estradiol in vitro. BMC Musculoskelet Disord. 3:42002. View Article : Google Scholar : PubMed/NCBI

30 

Weber A, Wandinger KP, Mueller W, Aktas O, Wengert O, Grundström E, Ehrlich S, Windemuth C, Kuhlmann T, Wienker T, et al: Identification and functional characterization of a highly polymorphic region in the human TRAIL promoter in multiple sclerosis. J Neuroimmunol. 149:195–201. 2004. View Article : Google Scholar : PubMed/NCBI

31 

Singh M, Sharma H and Singh N: Hydrogen peroxide induces apoptosis in HeLa cells through mitochondrial pathway. Mitochondrion. 7:367–373. 2007. View Article : Google Scholar : PubMed/NCBI

32 

He B, Tao H, Liu S and Wei A: Protective effect of carboxymethylated chitosan on hydrogen peroxide-induced apoptosis in nucleus pulposus cells. Mol Med Rep. 11:1629–1638. 2015.PubMed/NCBI

33 

Li Z, Jo J, Jia JM, Lo SC, Whitcomb DJ, Jiao S, Cho K and Sheng M: Caspase-3 activation via mitochondria is required for long-term depression and AMPA receptor internalization. Cell. 141:859–871. 2010. View Article : Google Scholar : PubMed/NCBI

34 

Sudo H and Minami A: Caspase 3 as a therapeutic target for regulation of intervertebral disc degeneration in rabbits. Arthritis Rheum. 63:1648–1657. 2011. View Article : Google Scholar : PubMed/NCBI

35 

Zhao H, Yenari MA, Cheng D, Sapolsky RM and Steinberg GK: Bcl-2 overexpression protects against neuron loss within the ischemic margin following experimental stroke and inhibits cytochrome c translocation and caspase-3 activity. J Neurochem. 85:1026–1036. 2003. View Article : Google Scholar : PubMed/NCBI

36 

Shakibaei M, John T, Seifarth C and Mobasheri A: Resveratrol inhibits IL-1 beta-induced stimulation of caspase-3 and cleavage of PARP in human articular chondrocytes in vitro. Ann N Y Acad Sci. 1095:554–563. 2007. View Article : Google Scholar : PubMed/NCBI

37 

Yang SD, Ma L, Yang DL and Ding WY: Combined effect of 17β-estradiol and resveratrol against apoptosis induced by interleukin-1β in rat nucleus pulposus cells via PI3K/Akt/caspase-3 pathway. PeerJ. 4:e16402016. View Article : Google Scholar : PubMed/NCBI

38 

Wesierska-Gadek J, Schreiner T, Gueorguieva M and Ranftler C: Phenol red reduces ROSC mediated cell cycle arrest and apoptosis in human MCF-7 cells. J Cell Biochem. 98:1367–1379. 2006. View Article : Google Scholar : PubMed/NCBI

39 

Weiler C, Nerlich AG, Bachmeier BE and Boos N: Expression and distribution of tumor necrosis factor alpha in human lumbar intervertebral discs: A study in surgical specimen and autopsy controls. Spine (Phila Pa 1976). 30:44–54. 2005. View Article : Google Scholar : PubMed/NCBI

40 

Wu Y and Zhou BP: TNF-alpha/NF-kappaB/Snail pathway in cancer cell migration and invasion. Br J Cancer. 102:639–644. 2010. View Article : Google Scholar : PubMed/NCBI

41 

Sandra F, Matsuki NA, Takeuchi H, Ikebe T, Kanematsu T, Ohishi M and Hirata M: TNF inhibited the apoptosis by activation of Akt serine/threonine kinase in the human head and neck squamous cell carcinoma. Cell Signal. 14:771–778. 2002. View Article : Google Scholar : PubMed/NCBI

42 

Zhao CM, Chen Q, Zhang WJ, Huang AB, Zhang W, Yang HL and Zhang ZM: 17β-estradiol protects rat annulus fibrosus cells against apoptosis via α1 integrin-mediated adhesion to type I collagen: An in-vitro study. Med Sci Monit. 22:1375–1383. 2016. View Article : Google Scholar : PubMed/NCBI

43 

Yang SD, Yang DL, Sun YP, Wang BL, Ma L, Feng SQ and Ding WY: 17β-estradiol protects against apoptosis induced by interleukin-1β in rat nucleus pulposus cells by down-regulating MMP-3 and MMP-13. Apoptosis. 20:348–357. 2015. View Article : Google Scholar : PubMed/NCBI

44 

Aggarwal BB, Shishodia S, Sandur SK, Pandey MK and Sethi G: Inflammation and cancer: How hot is the link? Biochem Pharmacol. 72:1605–1621. 2006. View Article : Google Scholar : PubMed/NCBI

45 

Schmiegel W, Roeder C, Schmielau J, Rodeck U and Kalthoff H: Tumor necrosis factor alpha induces the expression of transforming growth factor alpha and the epidermal growth factor receptor in human pancreatic cancer cells. Proc Natl Acad Sci USA. 90:863–867. 1993. View Article : Google Scholar : PubMed/NCBI

46 

Wang T, Yang SD, Liu S, Wang H, Liu H and Ding WY: 17β-estradiol inhibites tumor necrosis factor-a induced apoptosis of human nucleus pulposus cells via the PI3K/Akt pathway. Med Sci Monit. 22:4312–4322. 2016. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liu H, Yang SD, Xu Y, Ning SH, Wang T, Yang DL and Ding WY: Protective role of 17β-estradiol on tumor necrosis factor-α-induced apoptosis in human nucleus pulposus cells. Mol Med Rep 16: 1093-1100, 2017.
APA
Liu, H., Yang, S., Xu, Y., Ning, S., Wang, T., Yang, D., & Ding, W. (2017). Protective role of 17β-estradiol on tumor necrosis factor-α-induced apoptosis in human nucleus pulposus cells. Molecular Medicine Reports, 16, 1093-1100. https://doi.org/10.3892/mmr.2017.6690
MLA
Liu, H., Yang, S., Xu, Y., Ning, S., Wang, T., Yang, D., Ding, W."Protective role of 17β-estradiol on tumor necrosis factor-α-induced apoptosis in human nucleus pulposus cells". Molecular Medicine Reports 16.2 (2017): 1093-1100.
Chicago
Liu, H., Yang, S., Xu, Y., Ning, S., Wang, T., Yang, D., Ding, W."Protective role of 17β-estradiol on tumor necrosis factor-α-induced apoptosis in human nucleus pulposus cells". Molecular Medicine Reports 16, no. 2 (2017): 1093-1100. https://doi.org/10.3892/mmr.2017.6690
Copy and paste a formatted citation
x
Spandidos Publications style
Liu H, Yang SD, Xu Y, Ning SH, Wang T, Yang DL and Ding WY: Protective role of 17β-estradiol on tumor necrosis factor-α-induced apoptosis in human nucleus pulposus cells. Mol Med Rep 16: 1093-1100, 2017.
APA
Liu, H., Yang, S., Xu, Y., Ning, S., Wang, T., Yang, D., & Ding, W. (2017). Protective role of 17β-estradiol on tumor necrosis factor-α-induced apoptosis in human nucleus pulposus cells. Molecular Medicine Reports, 16, 1093-1100. https://doi.org/10.3892/mmr.2017.6690
MLA
Liu, H., Yang, S., Xu, Y., Ning, S., Wang, T., Yang, D., Ding, W."Protective role of 17β-estradiol on tumor necrosis factor-α-induced apoptosis in human nucleus pulposus cells". Molecular Medicine Reports 16.2 (2017): 1093-1100.
Chicago
Liu, H., Yang, S., Xu, Y., Ning, S., Wang, T., Yang, D., Ding, W."Protective role of 17β-estradiol on tumor necrosis factor-α-induced apoptosis in human nucleus pulposus cells". Molecular Medicine Reports 16, no. 2 (2017): 1093-1100. https://doi.org/10.3892/mmr.2017.6690
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