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Article

MicroRNA‑210 negatively regulates the radiosensitivity of nasopharyngeal carcinoma cells

  • Authors:
    • Bo‑Yi Li
    • Yi Luo
    • Wen‑Si Zhao
    • Lu Zhang
    • Han‑Jing Zhou
    • Yu‑Chun Zou
    • Tao Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Oncology, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China, Department of Oncology, Shizhu County People's Hospital, Chongqing 409100, P.R. China
  • Pages: 1401-1408
    |
    Published online on: June 6, 2017
       https://doi.org/10.3892/mmr.2017.6694
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Abstract

Radiotherapy is one of the primary methods of treatment of malignant tumors, however, resistance to radiation is a major problem. The reasons for the radioresistance are still poorly understood. However, it is generally accepted that microRNAs (miRNAs or miRs) can regulate the radiosensitivity of tumors. The present study therefore aimed to identify specific miRNAs and their effects on radioresistant cells. More specifically, the aim was to investigate specific miRNAs and their effects on radioresistant tumor cells. The radioresistant tumor cells (CNE‑2R) were established using a dose gradient method, and the miRNA expression profiles of CNE‑2R cells and the parental cells (CNE‑2) were determined. The expression of miR‑210 in CNE‑2R cells was significantly higher than in CNE‑2 cells. CNE‑2R cells were transfected with LV‑hsa‑miR‑210‑inhibitor, and CNE‑2 cells were transfected with LV‑hsa‑miR‑210. The expression of miR‑210 was confirmed by reverse transcription quantitative‑polymerase chain reaction. The percentages of CNE‑2R‑miR‑210‑inhibitor and CNE‑2 cells in the G2/M phase were higher than in the CNE‑2R and CNE‑2‑miR‑210 cells, and the percentages of cells in S phase were lower than in the CNE‑2R and CNE‑2‑miR‑210 cells. Following 4 Gy of radiation, CNE‑2R‑miR‑210‑inhibitor and CNE‑2 cells, which express low levels of miR‑210, had a higher apoptosis rate than CNE‑2R and CNE‑2‑miR‑210 cells. Following 4, 8 and 12 Gy of radiation, cell viability and survival fraction of CNE‑2R‑miR‑210‑inhibitor cells were lower than those of CNE‑2R and CNE‑2‑miR‑210 cells, and similar to those of CNE‑2 cells. Together, these findings strongly suggest that miR‑210 negatively regulates the radiosensitivity of tumor cells, and may therefore have therapeutic potential for the treatment of radiation resistance.
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Copy and paste a formatted citation
Spandidos Publications style
Li BY, Luo Y, Zhao WS, Zhang L, Zhou HJ, Zou YC and Zhang T: MicroRNA‑210 negatively regulates the radiosensitivity of nasopharyngeal carcinoma cells. Mol Med Rep 16: 1401-1408, 2017.
APA
Li, B., Luo, Y., Zhao, W., Zhang, L., Zhou, H., Zou, Y., & Zhang, T. (2017). MicroRNA‑210 negatively regulates the radiosensitivity of nasopharyngeal carcinoma cells. Molecular Medicine Reports, 16, 1401-1408. https://doi.org/10.3892/mmr.2017.6694
MLA
Li, B., Luo, Y., Zhao, W., Zhang, L., Zhou, H., Zou, Y., Zhang, T."MicroRNA‑210 negatively regulates the radiosensitivity of nasopharyngeal carcinoma cells". Molecular Medicine Reports 16.2 (2017): 1401-1408.
Chicago
Li, B., Luo, Y., Zhao, W., Zhang, L., Zhou, H., Zou, Y., Zhang, T."MicroRNA‑210 negatively regulates the radiosensitivity of nasopharyngeal carcinoma cells". Molecular Medicine Reports 16, no. 2 (2017): 1401-1408. https://doi.org/10.3892/mmr.2017.6694
Copy and paste a formatted citation
x
Spandidos Publications style
Li BY, Luo Y, Zhao WS, Zhang L, Zhou HJ, Zou YC and Zhang T: MicroRNA‑210 negatively regulates the radiosensitivity of nasopharyngeal carcinoma cells. Mol Med Rep 16: 1401-1408, 2017.
APA
Li, B., Luo, Y., Zhao, W., Zhang, L., Zhou, H., Zou, Y., & Zhang, T. (2017). MicroRNA‑210 negatively regulates the radiosensitivity of nasopharyngeal carcinoma cells. Molecular Medicine Reports, 16, 1401-1408. https://doi.org/10.3892/mmr.2017.6694
MLA
Li, B., Luo, Y., Zhao, W., Zhang, L., Zhou, H., Zou, Y., Zhang, T."MicroRNA‑210 negatively regulates the radiosensitivity of nasopharyngeal carcinoma cells". Molecular Medicine Reports 16.2 (2017): 1401-1408.
Chicago
Li, B., Luo, Y., Zhao, W., Zhang, L., Zhou, H., Zou, Y., Zhang, T."MicroRNA‑210 negatively regulates the radiosensitivity of nasopharyngeal carcinoma cells". Molecular Medicine Reports 16, no. 2 (2017): 1401-1408. https://doi.org/10.3892/mmr.2017.6694
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