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Protective effect of berberine on renal fibrosis caused by diabetic nephropathy

  • Authors:
    • Zhong Li
    • Wei Zhang
  • View Affiliations / Copyright

    Affiliations: The Clinical Teaching and Research Department, School of Zhang Zhongjing Traditional Chinese Medicine, Nanyang Institute of Technology, Nanyang, Henan 473004, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1055-1062
    |
    Published online on: June 7, 2017
       https://doi.org/10.3892/mmr.2017.6707
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Abstract

Berberine (BBR) is a material extracted from Chinese herbs, which has been used in the treatment of diabetes in Chinese medicine for thousands of years. However, the importance of BBR in renal fibrosis remains to be elucidated. In the present study, streptozotocin‑induced diabetic nephropathy (DN) rats were used to determine the effect of BBR on renal fibrosis. The pathology of the kidneys was examined using periodic acid‑Schiff (PAS) and Masson staining. The expression levels of transforming growth factor‑β (TGF‑β) and α smooth muscle actin (α‑SMA) in kidneys were observed using immunohistochemical staining. The mRNA and protein expression levels of TGF‑β, α‑SMA, vimentin, nuclear factor‑κB were examined using reverse transcription‑quantitative polymerase chain reaction and western blotting, respectively. PAS and Masson staining revealed that there was notable glomerular hypertrophy and mesangial matrix expansion in DN rats. Immunohistochemistry revealed that there was a significant increase in TGF‑β and α‑SMA expression levels in the renal tubulointerstitium and the extracellular matrix. However, treatment with BBR may significantly reduce kidney injury. The protein and mRNA expression levels of TGF‑β, vimentin and α‑SMA were significantly increased in DN rats compared with the control group; however, this increase was reduced following treatment with BBR. The present study revealed that BBR may inhibit fibrosis and ameliorate the symptoms of DN. The current findings indicated that BBR may be used as a potential treatment for patients with DN.
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Copy and paste a formatted citation
Spandidos Publications style
Li Z and Zhang W: Protective effect of berberine on renal fibrosis caused by diabetic nephropathy. Mol Med Rep 16: 1055-1062, 2017.
APA
Li, Z., & Zhang, W. (2017). Protective effect of berberine on renal fibrosis caused by diabetic nephropathy. Molecular Medicine Reports, 16, 1055-1062. https://doi.org/10.3892/mmr.2017.6707
MLA
Li, Z., Zhang, W."Protective effect of berberine on renal fibrosis caused by diabetic nephropathy". Molecular Medicine Reports 16.2 (2017): 1055-1062.
Chicago
Li, Z., Zhang, W."Protective effect of berberine on renal fibrosis caused by diabetic nephropathy". Molecular Medicine Reports 16, no. 2 (2017): 1055-1062. https://doi.org/10.3892/mmr.2017.6707
Copy and paste a formatted citation
x
Spandidos Publications style
Li Z and Zhang W: Protective effect of berberine on renal fibrosis caused by diabetic nephropathy. Mol Med Rep 16: 1055-1062, 2017.
APA
Li, Z., & Zhang, W. (2017). Protective effect of berberine on renal fibrosis caused by diabetic nephropathy. Molecular Medicine Reports, 16, 1055-1062. https://doi.org/10.3892/mmr.2017.6707
MLA
Li, Z., Zhang, W."Protective effect of berberine on renal fibrosis caused by diabetic nephropathy". Molecular Medicine Reports 16.2 (2017): 1055-1062.
Chicago
Li, Z., Zhang, W."Protective effect of berberine on renal fibrosis caused by diabetic nephropathy". Molecular Medicine Reports 16, no. 2 (2017): 1055-1062. https://doi.org/10.3892/mmr.2017.6707
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