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Article

Estradiol postconditioning relieves ischemia/reperfusion injury in axial skin flaps of rats, inhibits apoptosis and alters the MKP-1/ERK pathway

  • Authors:
    • Qianheng Jin
    • Jihui Ju
    • Lei Xu
    • Yuefei Liu
    • Zhimin Li
    • Yi Fu
    • Ruixing Hou
  • View Affiliations / Copyright

    Affiliations: Institute of Hand Surgery, Ruihua Affiliated Hospital of Soochow University, Suzhou, Jiangsu 215100, P.R. China, Department of Human Anatomy, Histology and Embryology, School of Biology and Basic Medical Sciences, Soochow University, Suzhou, Jiangsu 215100, P.R. China
  • Pages: 1472-1478
    |
    Published online on: June 7, 2017
       https://doi.org/10.3892/mmr.2017.6708
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Abstract

Previous studies have suggested that estradiol can reduce the ischemia/reperfusion (I/R) injury in skin flaps. However, the mechanism, particularly the signal pathways involved in this protective effect are not well established. In the current study, an I/R injury model was established in rats to explore the connection between estradiol protection during I/R injury and extracellular signal‑regulated kinase (ERK) signaling. Healthy male Wistar rats were divided into five groups (n=10): Control group (group I), I/R group (group II), saline group (group III), estradiol group (group IV) and inhibitor (PD‑98059) group (group V). The survival rate of the flap was compared between groups, morphological changes were observed by hematoxylin and eosin staining of sections, and terminal deoxynucleotidyl transferase dUTP nick end labeling was performed to identify apoptotic cells and determine the apoptotic index. To further investigate the mechanism, western blot analysis was performed to assess the protein level of ERK1/2, phospho‑ERK1/2, and mitogen‑activated protein kinase phosphatase 1 (MKP‑1). The results of the present study demonstrated that estradiol therapy can reduce I/R injury and decrease the apoptosis index in an axial skin flap model. The inhibitor of the ERK pathway (PD‑98059) partially abolished the effects of estradiol, which involve the phosphatase enzyme MKP‑1. Taken together, the findings of the present study indicate that estradiol may act by reducing the expression of MKP‑1, mediating the expression/activation changes of the ERK pathway and subsequently reduce the level of apoptosis and the I/R injury the axial flap. Estrogen may be used to mitigate the adverse reaction caused by ischemia‑reperfusion injury and effectively improve the survival rate and survival quality of free skin flap and improve patient prognosis.
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Copy and paste a formatted citation
Spandidos Publications style
Jin Q, Ju J, Xu L, Liu Y, Li Z, Fu Y and Hou R: Estradiol postconditioning relieves ischemia/reperfusion injury in axial skin flaps of rats, inhibits apoptosis and alters the MKP-1/ERK pathway. Mol Med Rep 16: 1472-1478, 2017.
APA
Jin, Q., Ju, J., Xu, L., Liu, Y., Li, Z., Fu, Y., & Hou, R. (2017). Estradiol postconditioning relieves ischemia/reperfusion injury in axial skin flaps of rats, inhibits apoptosis and alters the MKP-1/ERK pathway. Molecular Medicine Reports, 16, 1472-1478. https://doi.org/10.3892/mmr.2017.6708
MLA
Jin, Q., Ju, J., Xu, L., Liu, Y., Li, Z., Fu, Y., Hou, R."Estradiol postconditioning relieves ischemia/reperfusion injury in axial skin flaps of rats, inhibits apoptosis and alters the MKP-1/ERK pathway". Molecular Medicine Reports 16.2 (2017): 1472-1478.
Chicago
Jin, Q., Ju, J., Xu, L., Liu, Y., Li, Z., Fu, Y., Hou, R."Estradiol postconditioning relieves ischemia/reperfusion injury in axial skin flaps of rats, inhibits apoptosis and alters the MKP-1/ERK pathway". Molecular Medicine Reports 16, no. 2 (2017): 1472-1478. https://doi.org/10.3892/mmr.2017.6708
Copy and paste a formatted citation
x
Spandidos Publications style
Jin Q, Ju J, Xu L, Liu Y, Li Z, Fu Y and Hou R: Estradiol postconditioning relieves ischemia/reperfusion injury in axial skin flaps of rats, inhibits apoptosis and alters the MKP-1/ERK pathway. Mol Med Rep 16: 1472-1478, 2017.
APA
Jin, Q., Ju, J., Xu, L., Liu, Y., Li, Z., Fu, Y., & Hou, R. (2017). Estradiol postconditioning relieves ischemia/reperfusion injury in axial skin flaps of rats, inhibits apoptosis and alters the MKP-1/ERK pathway. Molecular Medicine Reports, 16, 1472-1478. https://doi.org/10.3892/mmr.2017.6708
MLA
Jin, Q., Ju, J., Xu, L., Liu, Y., Li, Z., Fu, Y., Hou, R."Estradiol postconditioning relieves ischemia/reperfusion injury in axial skin flaps of rats, inhibits apoptosis and alters the MKP-1/ERK pathway". Molecular Medicine Reports 16.2 (2017): 1472-1478.
Chicago
Jin, Q., Ju, J., Xu, L., Liu, Y., Li, Z., Fu, Y., Hou, R."Estradiol postconditioning relieves ischemia/reperfusion injury in axial skin flaps of rats, inhibits apoptosis and alters the MKP-1/ERK pathway". Molecular Medicine Reports 16, no. 2 (2017): 1472-1478. https://doi.org/10.3892/mmr.2017.6708
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