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Article

Synergistic regulatory effects of microRNAs on brain glioma cells

  • Authors:
    • Yilei Zhao
    • Xiaomeng Cui
    • Wenliang Zhu
    • Xin Chen
    • Chen Shen
    • Zhendong Liu
    • Guang Yang
    • Yaohua Liu
    • Shiguang Zhao
  • View Affiliations / Copyright

    Affiliations: Department of Pharmacy, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China, Institute of Measurement-Control Technology and Communications Engineering, Harbin University of Science and Technology, Harbin, Heilongjiang 150010, P.R. China, Institute of Clinical Pharmacology, The Second Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150086, P.R. China, Department of Neurosurgery, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China
  • Pages: 1409-1416
    |
    Published online on: June 7, 2017
       https://doi.org/10.3892/mmr.2017.6709
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Abstract

Glioma is among the most common types of cancer of the central nervous system and is difficult to cure. Due to the lack of glioma-specific treatments, patients with glioma exhibit high mortality rates. MicroRNAs (miRNAs) participate in the pathogenesis of glioma, and upregulation of specific miRNAs promotes cell proliferation, whereas apoptosis‑inducing miRNAs are markedly downregulated in the context of glioma. Therefore, miRNAs may be important contributors to the pathogenesis of glioma. In the present study, nine miRNAs were investigated as miRNA‑miRNA pairs, and the measured cell viabilities were consistent with the results of synergy predictions. Extensive synergy occurred among upregulated miRNAs in U87 cells, whereas downregulated miRNAs rarely exhibited synergism. Treatment with an miRNA‑miRNA pair exhibiting strong synergy increased the inhibitory effects of these miRNAs on tumor cells, and the combined inhibitory effects were increased compared with the sum of the individual inhibitory effects of each miRNA. Using cell viability assays, TUNEL staining, and flow cytometry, the present study demonstrates that cotransfection with miR‑20a and miR‑21inhibitors resulted in the highest synergistic effect on the promotion of apoptosis in U87 cells. The results of the present study provide important insights into the potential use of miRNAs in the treatment of glioma.
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Copy and paste a formatted citation
Spandidos Publications style
Zhao Y, Cui X, Zhu W, Chen X, Shen C, Liu Z, Yang G, Liu Y and Zhao S: Synergistic regulatory effects of microRNAs on brain glioma cells. Mol Med Rep 16: 1409-1416, 2017.
APA
Zhao, Y., Cui, X., Zhu, W., Chen, X., Shen, C., Liu, Z. ... Zhao, S. (2017). Synergistic regulatory effects of microRNAs on brain glioma cells. Molecular Medicine Reports, 16, 1409-1416. https://doi.org/10.3892/mmr.2017.6709
MLA
Zhao, Y., Cui, X., Zhu, W., Chen, X., Shen, C., Liu, Z., Yang, G., Liu, Y., Zhao, S."Synergistic regulatory effects of microRNAs on brain glioma cells". Molecular Medicine Reports 16.2 (2017): 1409-1416.
Chicago
Zhao, Y., Cui, X., Zhu, W., Chen, X., Shen, C., Liu, Z., Yang, G., Liu, Y., Zhao, S."Synergistic regulatory effects of microRNAs on brain glioma cells". Molecular Medicine Reports 16, no. 2 (2017): 1409-1416. https://doi.org/10.3892/mmr.2017.6709
Copy and paste a formatted citation
x
Spandidos Publications style
Zhao Y, Cui X, Zhu W, Chen X, Shen C, Liu Z, Yang G, Liu Y and Zhao S: Synergistic regulatory effects of microRNAs on brain glioma cells. Mol Med Rep 16: 1409-1416, 2017.
APA
Zhao, Y., Cui, X., Zhu, W., Chen, X., Shen, C., Liu, Z. ... Zhao, S. (2017). Synergistic regulatory effects of microRNAs on brain glioma cells. Molecular Medicine Reports, 16, 1409-1416. https://doi.org/10.3892/mmr.2017.6709
MLA
Zhao, Y., Cui, X., Zhu, W., Chen, X., Shen, C., Liu, Z., Yang, G., Liu, Y., Zhao, S."Synergistic regulatory effects of microRNAs on brain glioma cells". Molecular Medicine Reports 16.2 (2017): 1409-1416.
Chicago
Zhao, Y., Cui, X., Zhu, W., Chen, X., Shen, C., Liu, Z., Yang, G., Liu, Y., Zhao, S."Synergistic regulatory effects of microRNAs on brain glioma cells". Molecular Medicine Reports 16, no. 2 (2017): 1409-1416. https://doi.org/10.3892/mmr.2017.6709
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