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Article Open Access

Maresin 1 inhibits transforming growth factor-β1-induced proliferation, migration and differentiation in human lung fibroblasts

  • Authors:
    • Quanchao Sun
    • You Wu
    • Feng Zhao
    • Jianjun Wang
  • View Affiliations / Copyright

    Affiliations: Department of Thoracic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China, Department of Gastrointestinal Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, P.R. China
    Copyright: © Sun et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1523-1529
    |
    Published online on: June 7, 2017
       https://doi.org/10.3892/mmr.2017.6711
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Abstract

The myofibroblast has been implicated to be an important pathogenic cell in all fibrotic diseases, through synthesis of excess extracellular matrix. Lung fibroblast migration, proliferation and differentiation into a myofibroblast‑like cell type are regarded as important steps in the formation of lung fibrosis. In the present study, the effect of maresin 1 (MaR 1), a pro‑resolving lipid mediator, on transforming growth factor (TGF)‑β1‑stimulated lung fibroblasts was investigated, and the underlying molecular mechanisms were examined. The results of the present study demonstrated that MaR 1 inhibited TGF‑β1‑induced proliferative and migratory ability, assessed using MTT and scratch wound healing assays. The TGF‑β1‑induced expression of α‑smooth muscle actin (α‑SMA) and collagen type I, the hallmarks of myofibroblast differentiation, was decreased by MaR 1 at the mRNA and protein levels, determined using the reverse transcription‑quantitative polymerase chain reaction and western blot analysis, respectively. Immunofluorescence demonstrated that MaR 1 downregulated the TGF‑β1‑induced expression of α‑SMA. In addition, phosphorylated mothers against decapentaplegic homolog 2/3 (Smad2/3) and extracellular signal‑related kinases (ERK) 1/2 were upregulated in TGF-β1-induced lung fibroblasts, and these effects were attenuated by MaR 1 administration. In conclusion, the results of the present study demonstrated that MaR 1 inhibited the TGF‑β1‑induced proliferation, migration and differentiation of human lung fibroblasts. These observed effects may be mediated in part by decreased phosphorylation of Smad2/3 and ERK1/2 signaling pathways. Therefore, MaR 1 may be a potential therapeutic approach to lung fibrotic diseases.
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Copy and paste a formatted citation
Spandidos Publications style
Sun Q, Wu Y, Zhao F and Wang J: Maresin 1 inhibits transforming growth factor-β1-induced proliferation, migration and differentiation in human lung fibroblasts. Mol Med Rep 16: 1523-1529, 2017.
APA
Sun, Q., Wu, Y., Zhao, F., & Wang, J. (2017). Maresin 1 inhibits transforming growth factor-β1-induced proliferation, migration and differentiation in human lung fibroblasts. Molecular Medicine Reports, 16, 1523-1529. https://doi.org/10.3892/mmr.2017.6711
MLA
Sun, Q., Wu, Y., Zhao, F., Wang, J."Maresin 1 inhibits transforming growth factor-β1-induced proliferation, migration and differentiation in human lung fibroblasts". Molecular Medicine Reports 16.2 (2017): 1523-1529.
Chicago
Sun, Q., Wu, Y., Zhao, F., Wang, J."Maresin 1 inhibits transforming growth factor-β1-induced proliferation, migration and differentiation in human lung fibroblasts". Molecular Medicine Reports 16, no. 2 (2017): 1523-1529. https://doi.org/10.3892/mmr.2017.6711
Copy and paste a formatted citation
x
Spandidos Publications style
Sun Q, Wu Y, Zhao F and Wang J: Maresin 1 inhibits transforming growth factor-β1-induced proliferation, migration and differentiation in human lung fibroblasts. Mol Med Rep 16: 1523-1529, 2017.
APA
Sun, Q., Wu, Y., Zhao, F., & Wang, J. (2017). Maresin 1 inhibits transforming growth factor-β1-induced proliferation, migration and differentiation in human lung fibroblasts. Molecular Medicine Reports, 16, 1523-1529. https://doi.org/10.3892/mmr.2017.6711
MLA
Sun, Q., Wu, Y., Zhao, F., Wang, J."Maresin 1 inhibits transforming growth factor-β1-induced proliferation, migration and differentiation in human lung fibroblasts". Molecular Medicine Reports 16.2 (2017): 1523-1529.
Chicago
Sun, Q., Wu, Y., Zhao, F., Wang, J."Maresin 1 inhibits transforming growth factor-β1-induced proliferation, migration and differentiation in human lung fibroblasts". Molecular Medicine Reports 16, no. 2 (2017): 1523-1529. https://doi.org/10.3892/mmr.2017.6711
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