Downregulation of microRNA-448 improves isoflurane-induced learning and memory impairment in rats

  • Authors:
    • Qun Wu
    • Qinxue Dai
    • Liuming Jiang
    • Yichuan Wang
    • Tao Yang
    • Jianxia Miao
    • Junlu Wang
    • Yu Han
  • View Affiliations

  • Published online on: June 8, 2017     https://doi.org/10.3892/mmr.2017.6724
  • Pages: 1578-1583
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Abstract

The present study aimed to investigate the potential role of microRNA‑448 (miR‑448) in isoflurane-induced learning and memory impairment in rats. Sprague‑Dawley rats were used for the construction of isoflurane‑treated models. The Morris water maze test was used to evaluate the effects of isoflurane on rats regarding the following para­meters: Swimming speed, escape latency and time in original quadrant. Influences of isoflurane on neuron apoptosis and miR‑448 expression in rat hippocampus tissue were analyzed by flow cytometry and reverse transcription‑quantitative polymerase chain reaction, respectively. Furthermore, the effects of miR‑448 on the expression of cell apoptosis‑associated proteins were investigated by flow cytometry. The results demonstrated that isoflurane treatment induced higher escape latency and lower time spent in original quadrant compared with the control rats. In addition, isoflurane treatment induced neuron apoptosis and miR‑448 was highly expressed in the hippocampal tissue of isoflurane‑treated rats. Furthermore, Bcl‑x was significantly downregulated while caspase‑3 expression was upregulated by an miR‑448 inhibitor. Combined the results of the current study indicate that miR‑448 knockdown may have pivotal roles in improving isoflurane-induced learning and memory impairment via suppressing neuron apoptosis.
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August-2017
Volume 16 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Wu Q, Dai Q, Jiang L, Wang Y, Yang T, Miao J, Wang J and Han Y: Downregulation of microRNA-448 improves isoflurane-induced learning and memory impairment in rats. Mol Med Rep 16: 1578-1583, 2017
APA
Wu, Q., Dai, Q., Jiang, L., Wang, Y., Yang, T., Miao, J. ... Han, Y. (2017). Downregulation of microRNA-448 improves isoflurane-induced learning and memory impairment in rats. Molecular Medicine Reports, 16, 1578-1583. https://doi.org/10.3892/mmr.2017.6724
MLA
Wu, Q., Dai, Q., Jiang, L., Wang, Y., Yang, T., Miao, J., Wang, J., Han, Y."Downregulation of microRNA-448 improves isoflurane-induced learning and memory impairment in rats". Molecular Medicine Reports 16.2 (2017): 1578-1583.
Chicago
Wu, Q., Dai, Q., Jiang, L., Wang, Y., Yang, T., Miao, J., Wang, J., Han, Y."Downregulation of microRNA-448 improves isoflurane-induced learning and memory impairment in rats". Molecular Medicine Reports 16, no. 2 (2017): 1578-1583. https://doi.org/10.3892/mmr.2017.6724