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Hypoxia serves a key function in the upregulated expression of vascular adhesion protein‑1 in vitro and in a rat model of hemorrhagic shock

  • Authors:
    • Yuxing Zhang
    • Wei Yi
    • Jun Yao
    • Xiaojun Yu
    • Cheng Qian
    • Zhiqian Hu
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery, Navy General Hospital, Beijing 100048, P.R. China, Department of General Surgery, China People's Liberation Army No. 94 Hospital, Nanchang, Jiangxi 330002, P.R. China, Department of General Surgery, Shanghai Changzheng Hospital, Shanghai 200003, P.R. China, Department of Gastroenterological Surgery, Zhejiang Provincial People's Hospital, Hangzhou, Zhejiang 310014, P.R. China, Department of General Surgery, Huzhou Maternity & Child Care Hospital, Huzhou, Zhejiang 313000, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1189-1199
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    Published online on: June 8, 2017
       https://doi.org/10.3892/mmr.2017.6727
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Abstract

Hemorrhagic shock following major trauma results in mortality, but the function of vascular adhesion protein‑1 (VAP‑1), implicated in intracranial hemorrhage, remains unknown. This study aimed to determine whether expression of the AOC3 gene and its encoded protein, VAP‑1, is altered by hypoxia. Rat hepatic sinusoidal endothelial cells (RHSECs) and rat intestinal microvascular endothelial cells (RIMECs) were transduced with a viral vector carrying AOC3, and AOC3 mRNA expression levels were measured by reverse transcription‑quantitative polymerase chain reaction. VAP‑1 protein expression levels were measured by western blot analysis and compared between normoxic and hypoxic conditions. Following this, AOC3 mRNA and VAP‑1 protein expression levels in hepatic and intestinal tissues were assessed in a rat model of hemorrhagic shock with or without fluid resuscitation; and serum semicarbazide‑sensitive amine oxidase (SSAO) activity was measured by fluorometric assays. The effects of 2‑bromoethylamine (2‑BEA) on AOC3/VAP‑1 levels and 24 h survival were investigated. AOC3 mRNA and VAP‑1 protein levels were increased in RHSECs and RIMECs by hypoxia, and in hepatic and intestinal tissues from rats following hemorrhagic shock. Hypoxia increased serum SSAO activity in these animals. 2‑BEA reduced AOC3 mRNA and VAP‑1 protein levels in hepatic and intestinal tissues from rats following hemorrhagic shock, and appeared to improve survival in animals not receiving resuscitation following hemorrhagic shock. In conclusion, hemorrhagic shock upregulates AOC3/VAP‑1 expressions, and this potentially occurs via hypoxia. Therefore, inhibition of VAP‑1 may be beneficial in the setting of hemorrhagic shock. Further studies are required to confirm these findings and to establish whether VAP‑1 may be a valid target for the development of novel therapies for hemorrhagic shock.
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Spandidos Publications style
Zhang Y, Yi W, Yao J, Yu X, Qian C and Hu Z: Hypoxia serves a key function in the upregulated expression of vascular adhesion protein‑1 in vitro and in a rat model of hemorrhagic shock. Mol Med Rep 16: 1189-1199, 2017.
APA
Zhang, Y., Yi, W., Yao, J., Yu, X., Qian, C., & Hu, Z. (2017). Hypoxia serves a key function in the upregulated expression of vascular adhesion protein‑1 in vitro and in a rat model of hemorrhagic shock. Molecular Medicine Reports, 16, 1189-1199. https://doi.org/10.3892/mmr.2017.6727
MLA
Zhang, Y., Yi, W., Yao, J., Yu, X., Qian, C., Hu, Z."Hypoxia serves a key function in the upregulated expression of vascular adhesion protein‑1 in vitro and in a rat model of hemorrhagic shock". Molecular Medicine Reports 16.2 (2017): 1189-1199.
Chicago
Zhang, Y., Yi, W., Yao, J., Yu, X., Qian, C., Hu, Z."Hypoxia serves a key function in the upregulated expression of vascular adhesion protein‑1 in vitro and in a rat model of hemorrhagic shock". Molecular Medicine Reports 16, no. 2 (2017): 1189-1199. https://doi.org/10.3892/mmr.2017.6727
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y, Yi W, Yao J, Yu X, Qian C and Hu Z: Hypoxia serves a key function in the upregulated expression of vascular adhesion protein‑1 in vitro and in a rat model of hemorrhagic shock. Mol Med Rep 16: 1189-1199, 2017.
APA
Zhang, Y., Yi, W., Yao, J., Yu, X., Qian, C., & Hu, Z. (2017). Hypoxia serves a key function in the upregulated expression of vascular adhesion protein‑1 in vitro and in a rat model of hemorrhagic shock. Molecular Medicine Reports, 16, 1189-1199. https://doi.org/10.3892/mmr.2017.6727
MLA
Zhang, Y., Yi, W., Yao, J., Yu, X., Qian, C., Hu, Z."Hypoxia serves a key function in the upregulated expression of vascular adhesion protein‑1 in vitro and in a rat model of hemorrhagic shock". Molecular Medicine Reports 16.2 (2017): 1189-1199.
Chicago
Zhang, Y., Yi, W., Yao, J., Yu, X., Qian, C., Hu, Z."Hypoxia serves a key function in the upregulated expression of vascular adhesion protein‑1 in vitro and in a rat model of hemorrhagic shock". Molecular Medicine Reports 16, no. 2 (2017): 1189-1199. https://doi.org/10.3892/mmr.2017.6727
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