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MicroRNA‑7‑5p regulates the expression of TFF3 in inflammatory bowel disease

  • Authors:
    • Jing Guo
    • Mei Sun
    • Xu Teng
    • Lingfen Xu
  • View Affiliations / Copyright

    Affiliations: Department of Pediatrics, The Affiliated Shengjing Hospital, China Medical University, Shenyang, Liaoning 110004, P.R. China
    Copyright: © Guo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1200-1206
    |
    Published online on: June 8, 2017
       https://doi.org/10.3892/mmr.2017.6730
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Abstract

Trefoil factor 3 (TFF3) serves an important role in intestinal mucosal damage and healing, and contributes to the pathogenesis and treatment of inflammatory bowel disease (IBD). The aim of the present study was to determine the association between TFF3 and microRNA‑7‑5p (miR‑7‑5p) in IBD. Tissue immunohistochemistry was applied to evaluate the relative expression of TFF3, and reverse transcription‑quantitative polymerase chain reaction was performed to determine the expression of miR‑7‑5p in lesional tissue obtained from patients with IBD and healthy control tissues. A dual‑luciferase reporter assay was used to investigate whether TFF3 was a target of miR‑7‑5p, and western blotting was performed to determine the expression of TFF3 when miR‑7‑5p was overexpressed or suppressed. The protein expression levels of TFF3 were decreased and miR‑7‑5p was overexpressed in the lesional tissue of patients with IBD compared with in healthy control tissues. TFF3 was identified as a target of miR‑7‑5p, and TFF3 protein expression was negatively regulated by miR‑7‑5p in human colonic epithelial LS174T cells. The present study demonstrated a negative association between the expression of miR‑7‑5p and TFF3 in IBD lesional tissues and normal tissues. In conclusion, TFF3 was identified as a novel target of miR‑7‑5p and miR‑7‑5p may serve as a promising therapeutic target for IBD.
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Copy and paste a formatted citation
Spandidos Publications style
Guo J, Sun M, Teng X and Xu L: MicroRNA‑7‑5p regulates the expression of TFF3 in inflammatory bowel disease. Mol Med Rep 16: 1200-1206, 2017.
APA
Guo, J., Sun, M., Teng, X., & Xu, L. (2017). MicroRNA‑7‑5p regulates the expression of TFF3 in inflammatory bowel disease. Molecular Medicine Reports, 16, 1200-1206. https://doi.org/10.3892/mmr.2017.6730
MLA
Guo, J., Sun, M., Teng, X., Xu, L."MicroRNA‑7‑5p regulates the expression of TFF3 in inflammatory bowel disease". Molecular Medicine Reports 16.2 (2017): 1200-1206.
Chicago
Guo, J., Sun, M., Teng, X., Xu, L."MicroRNA‑7‑5p regulates the expression of TFF3 in inflammatory bowel disease". Molecular Medicine Reports 16, no. 2 (2017): 1200-1206. https://doi.org/10.3892/mmr.2017.6730
Copy and paste a formatted citation
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Spandidos Publications style
Guo J, Sun M, Teng X and Xu L: MicroRNA‑7‑5p regulates the expression of TFF3 in inflammatory bowel disease. Mol Med Rep 16: 1200-1206, 2017.
APA
Guo, J., Sun, M., Teng, X., & Xu, L. (2017). MicroRNA‑7‑5p regulates the expression of TFF3 in inflammatory bowel disease. Molecular Medicine Reports, 16, 1200-1206. https://doi.org/10.3892/mmr.2017.6730
MLA
Guo, J., Sun, M., Teng, X., Xu, L."MicroRNA‑7‑5p regulates the expression of TFF3 in inflammatory bowel disease". Molecular Medicine Reports 16.2 (2017): 1200-1206.
Chicago
Guo, J., Sun, M., Teng, X., Xu, L."MicroRNA‑7‑5p regulates the expression of TFF3 in inflammatory bowel disease". Molecular Medicine Reports 16, no. 2 (2017): 1200-1206. https://doi.org/10.3892/mmr.2017.6730
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