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Gene expression profiling of human blastocysts from in vivo and ‘rescue IVM’ with or without melatonin treatment

  • Authors:
    • Yan Hao
    • Zhiguo Zhang
    • Dan Han
    • Yunxia Cao
    • Ping Zhou
    • Zhaolian Wei
    • Mingrong Lv
    • Dawei Chen
  • View Affiliations / Copyright

    Affiliations: Department of Obstetrics and Gynecology, Reproductive Medicine Center, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230032, P.R. China
    Copyright: © Hao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1278-1288
    |
    Published online on: June 9, 2017
       https://doi.org/10.3892/mmr.2017.6742
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Abstract

To evaluate the effect of melatonin supplementation in maturation medium for human ‘rescue IVM’ and investigate differences in transcriptomic profile of blastocysts developed from oocytes matured in vitro with/without melatonin treatment and in vivo, a total of 314 GV oocytes and 320 MI oocytes were collected from 200 patients younger than 35 years old undergoing ICSI cycle. The oocytes were randomly distributed in the control group (no melatonin) and four other groups of varying melatonin concentrations (10‑11, 10‑9, 10‑7, 10‑5 mol/l). Gene profiling was performed on blastocysts developed from in vivo maturation oocytes (in vivo group), and in vitro maturation (IVM) oocytes with an optimal concentration of melatonin treatment (IVM‑anti group) or without melatonin (IVM group). The ratio of high quality blastocysts was significantly higher in the groups treated with 10‑5 mol/l melatonin compared with others groups. The large‑scale analysis of the transcriptome revealed significant differences in mRNA expression levels. In each group, nine blastocysts were selected for gene expression profiling. The differentially expressed genes were involved in cysteine and methionine metabolism, regulation of apoptotic process, mineral absorption, steroid hormone biosynthesis, Wnt signaling, p53 signaling pathway and other functions. The findings indicated that the IVM procedure may potentially affect DNA methylation and the canonical Wnt signaling pathway. Exogenous melatonin positively influenced quality of blastocysts, which may be mediated via upregulation of p53 signaling and correcting DNA methylation changes caused by ‘rescue IVM’. However, this study reflected what was generally referred to as ‘rescue IVM’ and was not a true reflection of clinical IVM techniques. Therefore, melatonin required further investigation as a promising supplement for use in IVM.
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Copy and paste a formatted citation
Spandidos Publications style
Hao Y, Zhang Z, Han D, Cao Y, Zhou P, Wei Z, Lv M and Chen D: Gene expression profiling of human blastocysts from in vivo and ‘rescue IVM’ with or without melatonin treatment. Mol Med Rep 16: 1278-1288, 2017.
APA
Hao, Y., Zhang, Z., Han, D., Cao, Y., Zhou, P., Wei, Z. ... Chen, D. (2017). Gene expression profiling of human blastocysts from in vivo and ‘rescue IVM’ with or without melatonin treatment. Molecular Medicine Reports, 16, 1278-1288. https://doi.org/10.3892/mmr.2017.6742
MLA
Hao, Y., Zhang, Z., Han, D., Cao, Y., Zhou, P., Wei, Z., Lv, M., Chen, D."Gene expression profiling of human blastocysts from in vivo and ‘rescue IVM’ with or without melatonin treatment". Molecular Medicine Reports 16.2 (2017): 1278-1288.
Chicago
Hao, Y., Zhang, Z., Han, D., Cao, Y., Zhou, P., Wei, Z., Lv, M., Chen, D."Gene expression profiling of human blastocysts from in vivo and ‘rescue IVM’ with or without melatonin treatment". Molecular Medicine Reports 16, no. 2 (2017): 1278-1288. https://doi.org/10.3892/mmr.2017.6742
Copy and paste a formatted citation
x
Spandidos Publications style
Hao Y, Zhang Z, Han D, Cao Y, Zhou P, Wei Z, Lv M and Chen D: Gene expression profiling of human blastocysts from in vivo and ‘rescue IVM’ with or without melatonin treatment. Mol Med Rep 16: 1278-1288, 2017.
APA
Hao, Y., Zhang, Z., Han, D., Cao, Y., Zhou, P., Wei, Z. ... Chen, D. (2017). Gene expression profiling of human blastocysts from in vivo and ‘rescue IVM’ with or without melatonin treatment. Molecular Medicine Reports, 16, 1278-1288. https://doi.org/10.3892/mmr.2017.6742
MLA
Hao, Y., Zhang, Z., Han, D., Cao, Y., Zhou, P., Wei, Z., Lv, M., Chen, D."Gene expression profiling of human blastocysts from in vivo and ‘rescue IVM’ with or without melatonin treatment". Molecular Medicine Reports 16.2 (2017): 1278-1288.
Chicago
Hao, Y., Zhang, Z., Han, D., Cao, Y., Zhou, P., Wei, Z., Lv, M., Chen, D."Gene expression profiling of human blastocysts from in vivo and ‘rescue IVM’ with or without melatonin treatment". Molecular Medicine Reports 16, no. 2 (2017): 1278-1288. https://doi.org/10.3892/mmr.2017.6742
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