Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
August-2017 Volume 16 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
August-2017 Volume 16 Issue 2

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

Eriodictyol 7‑O‑β‑D glucopyranoside from Coreopsis tinctoria Nutt. ameliorates lipid disorders via protecting mitochondrial function and suppressing lipogenesis

  • Authors:
    • Yuyan Liang
    • Hai Niu
    • Limei Ma
    • Dan Du
    • Li Wen
    • Qing Xia
    • Wen Huang
  • View Affiliations / Copyright

    Affiliations: Laboratory of Ethnopharmacology, Department of Integrated Traditional Chinese and Western Medicine, Regenerative Medicine Research Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, Sichuan 610041, P.R. China
    Copyright: © Liang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Pages: 1298-1306
    |
    Published online on: June 9, 2017
       https://doi.org/10.3892/mmr.2017.6743
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Coreopsis tinctoria (snow chrysanthemum) has been reported to exert antihyperlipidemic effects. The present study aimed to identify the active compounds of Coreopsis tinctoria and to investigate the molecular mechanisms underlying its effects on lipid dysregulation by measuring lipid levels, reactive oxygen species, lipid peroxidation and fatty acid synthesis. The present results demonstrated that snow chrysanthemum aqueous extracts significantly reduced serum lipid levels and oxidative stress in vivo. The main compounds that were isolated were identified as flavanomarein (compound 1) and eriodictyol 7‑O‑β‑D glucopyranoside (compound 2). Compounds 1 and 2 demonstrated potent antioxidative properties, including free radical scavenging activity, inhibition of lipid peroxidation, as well as lipid‑lowering effects in human HepG2 hepatocellular carcinoma cells treated with free fatty acids (FFAs). Compound 2 was revealed to suppress the elevation of triglyceride levels and inhibit lipid peroxidation following FFA treatment. In addition, it was demonstrated to significantly reduce intracellular levels of reactive oxygen species and improve the mitochondrial membrane potential and adenosine triphosphate levels, thus protecting mitochondrial function in FFA‑treated HepG2 cells. Furthermore, compound 2 markedly suppressed the protein expression levels of disulfide‑isomerase A3 precursor and fatty acid synthase, thus suppressing FFA‑induced lipogenesis in HepG2 cells. In conclusion, the present study identified compound 2 as one of the main active compounds in Coreopsis tinctoria responsible for its lipid‑lowering effects. Compound 2 was revealed to possess antihyperlipidemic properties, exerted via reducing oxidative stress, protecting mitochondrial function and suppressing lipogenesis.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

View References

1 

Abliz A, Aji Q, Abdusalam E, Sun X, Abdurahman A, Zhou W, Moore N and Umar A: Effect of Cydonia oblonga Mill. leaf extract on serum lipids and liver function in a rat model of hyperlipidaemia. J Ethnopharmacol. 151:970–974. 2014. View Article : Google Scholar : PubMed/NCBI

2 

Cignarella A, Bellosta S, Corsini A and Bolego C: Hypolipidemic therapy for the metabolic syndrome. Pharmacol Res. 53:492–500. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Luedde T, Kaplowitz N and Schwabe RF: Cell death and cell death responses in liver disease: Mechanisms and clinical relevance. Gastroenterology. 147:765–783. 2014. View Article : Google Scholar : PubMed/NCBI

4 

Irudayaraj SS, Sunil C, Duraipandiyan V and Ignacimuthu S: In vitro antioxidant and antihyperlipidemic activities of Toddaliaasiatica (L) Lam. Leaves in Triton WR-1339 and high fat diet induced hyperlipidemic rats. Food Chem Toxicol. 60:135–140. 2013. View Article : Google Scholar : PubMed/NCBI

5 

Zhang Y, Shi S, Zhao M, Chai X and Tu P: Coreosides A-D, C14-polyacetylene glycosides from the capitula of Coreopsis tinctoria and its anti-inflammatory activity against COX-2. Fitoterapia. 87:93–97. 2013. View Article : Google Scholar : PubMed/NCBI

6 

Zhang Y, Mourboul A and Li ZY: Research advance in medicinal plants from genus Coreopsis. Zhongguo Zhong Yao Za Zhi. 38:2633–2638. 2013.(In Chinese). PubMed/NCBI

7 

Dias T, Bronze MR, Houghton PJ, Mota-Filipe H and Paulo A: The flavonoid-rich fraction of Coreopsis tinctoria promotes glucose tolerance regain through pancreatic function recovery in streptozotocin-induced glucose-intolerant rats. J Ethnopharmacol. 132:483–490. 2010. View Article : Google Scholar : PubMed/NCBI

8 

Dias T, Liu B, Jones P, Houghton PJ, Mota-Filipe H and Paulo A: Cytoprotective effect of Coreopsis tinctoria extracts and flavonoids on tBHP and cytokine-induced cell injury in pancreatic MIN6 cells. J Ethnopharmacol. 139:485–492. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Li YL, Chen X, Xue J, Liu J, Chen X and Wulasihan M: Flavonoids furom Coreopsis tinctoria adjust lipid metabolism in hyperlipidemia animals by down-regulating adipose differentiation-related protein. Lipids Health Dis. 13:1932014. View Article : Google Scholar : PubMed/NCBI

10 

Gong G, Qin Y, Huang W, Zhou S, Wu X, Yang X, Zhao Y and Li D: Protective effects of diosgenin in the hyperlipidemic rat model and in human vascular endothelial cells against hydrogen peroxide-induced apoptosis. Chem-Biol Interact. 184:366–375. 2010. View Article : Google Scholar : PubMed/NCBI

11 

El-Demerdash FM and Nasr HM: Antioxidant effect of selenium on lipid peroxidation, hyperlipidemia and biochemical parameters in rats exposed to diazinon. J Trace Elem Med Bio. 28:89–93. 2014. View Article : Google Scholar

12 

Miri R, Saadati H, Ardi P and Firuzi O: Alterations in oxidative stress biomarkers associated with mild hyperlipidemia and smoking. Food Chem Toxicol. 50:920–926. 2012. View Article : Google Scholar : PubMed/NCBI

13 

Zarzecki MS, Araujo SM, Bortolotto VC, de Paula MT, Jesse RJ and Prigol M: Hypolipidemic action of chrysin on Triton WR-1339-induced hyperlipidemia in female C57BL/6 mice. Toxicol Rep. 1:200–208. 2014. View Article : Google Scholar

14 

Kim JS, Kang OJ and Gweon OC: Comparison of phenolic acids and flavonoids in black garlic at different thermal processing steps. J Funct Foods. 5:80–86. 2013. View Article : Google Scholar

15 

Gerhäuser C, Klimo K, Heiss E, Neumann I, Gamal-Eldeen A, Knauft J, Liu GY, Sitthimonchai S and Frank N: Mechanism-based in vitro screening of potential cancer chemopreventive agents. Mutat Res 523–524. 163–172. 2003. View Article : Google Scholar

16 

Hajiaghaalipour F, Kanthimathi MS, Sanusi J and Rajarajeswaran J: White tea (Camellia sinensis) inhibits proliferation of the colon cancer cell line, HT-29, activates caspases and protects DNA of normal cells against oxidative damage. Food Chem. 169:401–410. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Grespan R, Aguiar RP, Giubilei FN, Fuso RR, Damião MJ, Silva EL, Mikcha JG, Hernandes L, Amado C Bersani and Cuman RK: Hepatoprotective effect of pretreatment with Thymus vulgar is Essential Oil in experimental model of acetaminophen-induced injury. Evid Based Complement Alternat Med. 2014:9541362014. View Article : Google Scholar : PubMed/NCBI

18 

Seo MS, Hong SW, Yeon SH, Kim YM, Um KA, Kim JH, Kim HJ, Chang KC and Park SW: Magnolia officinalis attenuates free fatty acid-induced lipogenesis via AMPK phosphorylation in hepatocytes. J Ethnopharmacol. 157:140–148. 2014. View Article : Google Scholar : PubMed/NCBI

19 

Cui W, Chen SL and Hu KQ: Quantification and mechanisms of oleic acid-induced steatosis in HepG2 cells. Am J Transl Res. 2:95–104. 2010.PubMed/NCBI

20 

Sung DK, Chang YS, Kang S, Song HY, Park WS and Lee BH: Comparative evaluation of hypoxic-ischemic brain injury by flow cytometric analysis of mitochondrial membrane potential with JC-1 in neonatal rats. J Neurosci Methods. 193:232–238. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Gyamfi D, Everitt HE, Tewfik I, Clemens DL and Patel VB: Hepatic mitochondrial dysfunction induced by fatty acids and ethanol. Free Radic Biol Med. 53:2131–2145. 2012. View Article : Google Scholar : PubMed/NCBI

22 

Pan J, Zhang S, Yan L, Tai J, Xiao Q, Zou K, Zhou Y and Wu J: Separation of flavanone enantiomers and flavanone glucoside diastereomers from Balanophora involucrata Hook. F. by capillary electrophoresis and reversed-phase high-performance liquid chromatography on a C18 column. J Chromatogr A. 1185:117–129. 2008. View Article : Google Scholar : PubMed/NCBI

23 

Zhang Y, Shi S, Zhao M and Tu P: Novel chalcone from Coreopsis tinctoria Nutt. Biochem Systemat Ecol. 34:766–769. 2006. View Article : Google Scholar

24 

Czakó L, Szabolcs A, Vajda A, Csáti S, Venglovecz V, Rakonczay Z Jr, Hegyi P, Tiszlavicz L, Csont T, Pósa A, et al: Hyperlipidemia induced by a cholesterol-rich diet aggravates necrotizing pancreatitis in rats. Eur J Pharmacol. 572:74–81. 2007. View Article : Google Scholar : PubMed/NCBI

25 

Feng LJ, Yu CH, Ying KJ, Hua J and Dai XY: Hypolipidemic and antioxidant effects of total flavonoids of Perilla frutescens leaves in hyperlipidemia rats induced by high-fat diet. Food Res Int. 44:404–409. 2011. View Article : Google Scholar

26 

Neuschwander-Tetri BA: Hepatic lipotoxicity and the pathogenesis of nonalcoholic steatohepatitis: The central role of nontriglyceride fatty acid metabolites. Hepatology. 52:774–788. 2010. View Article : Google Scholar : PubMed/NCBI

27 

Zhao Y, Peng L, Lu W, Wang Y, Huang X, Gong C, He L, Hong J, Wu S and Jin X: Effect of Eclipta prostrata on lipid metabolism in hyperlipidemic animals. Exp Gerontol. 62:37–44. 2015. View Article : Google Scholar : PubMed/NCBI

28 

Wu X, Zhang L, Gurley E, Studer E, Shang J, Wang T, Wang C, Yan M, Jiang Z, Hylemon PB, et al: Prevention of free fatty acid-induced hepatic lipotoxicity by 18beta-glycyrrhetinic acid through lysosomal and mitochondrial pathways. Hepatology. 47:1905–1915. 2008. View Article : Google Scholar : PubMed/NCBI

29 

Schönfeld P, Więckowski MR, Lebiedzińska M and Wojtczak L: Mitochondrial fatty acid oxidation and oxidative stress: Lack of reverse electron transfer-associated production of reactive oxygen species. Biochim Biophys Acta. 1797:929–938. 2010. View Article : Google Scholar : PubMed/NCBI

30 

Schönfeld P and Wojtczak L: Fatty acids as modulators of the cellular production of reactive oxygen species. Free Radic Biol Med. 45:231–241. 2008. View Article : Google Scholar : PubMed/NCBI

31 

Wang H, Chan PK, Pan SY, Kwon KH, Ye Y, Chu JH, Fong WF, Tsui WM and Yu ZL: ERp57 is up-regulated in free fatty acids-induced steatotic L-02 cells and human nonalcoholic fatty livers. J Cell Biochem. 110:1447–1456. 2010. View Article : Google Scholar : PubMed/NCBI

32 

Menendez JA and Lupu R: Fatty acid synthase and the lipogenic phenotype in cancer pathogenesis. Nat Rev Cancer. 7:763–777. 2007. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Liang Y, Niu H, Ma L, Du D, Wen L, Xia Q and Huang W: Eriodictyol 7‑O‑β‑D glucopyranoside from Coreopsis tinctoria Nutt. ameliorates lipid disorders via protecting mitochondrial function and suppressing lipogenesis. Mol Med Rep 16: 1298-1306, 2017.
APA
Liang, Y., Niu, H., Ma, L., Du, D., Wen, L., Xia, Q., & Huang, W. (2017). Eriodictyol 7‑O‑β‑D glucopyranoside from Coreopsis tinctoria Nutt. ameliorates lipid disorders via protecting mitochondrial function and suppressing lipogenesis. Molecular Medicine Reports, 16, 1298-1306. https://doi.org/10.3892/mmr.2017.6743
MLA
Liang, Y., Niu, H., Ma, L., Du, D., Wen, L., Xia, Q., Huang, W."Eriodictyol 7‑O‑β‑D glucopyranoside from Coreopsis tinctoria Nutt. ameliorates lipid disorders via protecting mitochondrial function and suppressing lipogenesis". Molecular Medicine Reports 16.2 (2017): 1298-1306.
Chicago
Liang, Y., Niu, H., Ma, L., Du, D., Wen, L., Xia, Q., Huang, W."Eriodictyol 7‑O‑β‑D glucopyranoside from Coreopsis tinctoria Nutt. ameliorates lipid disorders via protecting mitochondrial function and suppressing lipogenesis". Molecular Medicine Reports 16, no. 2 (2017): 1298-1306. https://doi.org/10.3892/mmr.2017.6743
Copy and paste a formatted citation
x
Spandidos Publications style
Liang Y, Niu H, Ma L, Du D, Wen L, Xia Q and Huang W: Eriodictyol 7‑O‑β‑D glucopyranoside from Coreopsis tinctoria Nutt. ameliorates lipid disorders via protecting mitochondrial function and suppressing lipogenesis. Mol Med Rep 16: 1298-1306, 2017.
APA
Liang, Y., Niu, H., Ma, L., Du, D., Wen, L., Xia, Q., & Huang, W. (2017). Eriodictyol 7‑O‑β‑D glucopyranoside from Coreopsis tinctoria Nutt. ameliorates lipid disorders via protecting mitochondrial function and suppressing lipogenesis. Molecular Medicine Reports, 16, 1298-1306. https://doi.org/10.3892/mmr.2017.6743
MLA
Liang, Y., Niu, H., Ma, L., Du, D., Wen, L., Xia, Q., Huang, W."Eriodictyol 7‑O‑β‑D glucopyranoside from Coreopsis tinctoria Nutt. ameliorates lipid disorders via protecting mitochondrial function and suppressing lipogenesis". Molecular Medicine Reports 16.2 (2017): 1298-1306.
Chicago
Liang, Y., Niu, H., Ma, L., Du, D., Wen, L., Xia, Q., Huang, W."Eriodictyol 7‑O‑β‑D glucopyranoside from Coreopsis tinctoria Nutt. ameliorates lipid disorders via protecting mitochondrial function and suppressing lipogenesis". Molecular Medicine Reports 16, no. 2 (2017): 1298-1306. https://doi.org/10.3892/mmr.2017.6743
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team